1. parts.mit.edu iGEM & Synthetic Biology International Genetically Engineered Machine Competition Randy Rettberg igem2007.comparts.mit.edu SB 3.0 Zurich 6/24/07

2. parts.mit.edu An Engineering Question Can simple biological systems be built from standard, interchangable parts and operated in living cells? Or, is biology so complex that each case is unique?

3. parts.mit.edu Education Driving Research

4. parts.mit.edu International Genetically Engineered Machine Competition 2005 −13 Schools CaltechMITUC Berkeley Cambridge, UKOklahomaUCSF DavidsonPenn StateUT Austin ETH, ZurichPrinceton HarvardToronto, Canada −Sponsored by Microsoft/MIT iCampus

5. parts.mit.edu iGEM 2006 40 Schools, 33 Teams 450 participants Europe, Canada, Asia, Latin America, US Some EU funding in place iGEM ambassadors for US, Asia, and Europe Jamboree November 4 & 5 www.iGEM2006.com Supported by Microsoft Research and iCampus

6. parts.mit.edu Ege University, Turkey iGEM 2006

7. parts.mit.edu www.iGEM2006.com

8. parts.mit.edu Meet the Teams

9. parts.mit.edu iGEM Site Valencia iGEM Site

10. parts.mit.edu iGEM 2006 Results

11. parts.mit.edu Sample iGEM Projects  BerkeleyControlled Communications  DavidsonFlipping DNA  HarvardDNA Boxes  ETH ZurichPattern Matching  LjubljanaControlling Sepsis  Chiba JapanLight-Directed Chemotaxis  MississippiHydrogen Gas Detector  MITBanana and Wintergreen Smells  EdinburghArsenic Detector

12. parts.mit.edu MIT iGEM 2006 eau d’e coli mit igem 2006

13. The field test device A test tube could contain all the necessary components: Freeze dried bacteria, growth medium, indicator powder, Ampicillin salt, etc … These tubes could then be given to local villagers to monitor their own water quality themselves A good alternative to the widely used Gutzeit method www.Macteria.co.uk

14. parts.mit.edu Some Engineering Answers Can simple biological systems be built from standard, interchangable parts and operated in living cells? - YES, SOMETIMES Or, is biology so complex that each case is unique? - NOT ALWAYS

15. parts.mit.edu www.igem2007.com

16. parts.mit.edu Financial Resources Small Sample: $20K8 $30K8 $40K1 $50K4 >$50K2

17. parts.mit.edu 2008: 100 Schools -> Partition? Geographic Competitions Tracts Health and Medicine Energy and the Environment Parts and Devices Fun and Games Sensors Other iGEM 2007 and Beyond

18. parts.mit.edu The Impact of iGEM Students Making future synthetic biologists Teaching graceful competition Instructors Opportunities for junior faculty New programs - new ideas A task worth the effort Schools Synthetic biology entering curriculum Energize research programs Synthetic Biology Examples, parts, successes, testamonials

19. parts.mit.edu Registry of Standard Biological Parts An industry based on standard parts requires catalogues and suppliers of those parts.

20. parts.mit.edu http://parts.mit.edu Registry Registry Parts Catalog

21. parts.mit.edu Registry Contents 7/2007 Promoters Protein Coding Reporters RNA Terminators Signaling Many project parts 1400 Parts Available as DNA

22. parts.mit.edu Constitutive Promoters - Berkeley Part Characterization

23. parts.mit.edu DNA Repository

24. parts.mit.edu DNA Repository All parts sent to teams Parts are from previous teams

25. parts.mit.edu Robotic Assembly

26. parts.mit.edu DNA Synthesis Offer GENEART synthetic DNA offer for iGEM teams All you want at $0.70 per base 100,000 bases total at $0.25 per base About 2000 bases per team for $500 GENEART is a primary sponsor of iGEM 2007 http://www.geneart.com/index.php?id=252

27. parts.mit.edu New Programs BioBrick Part Program iGEM in Europe Registry in Europe New Registry Workshop Standards Workshop iGEM Steering Committee

28. parts.mit.edu Standards Device Families Protein : DNA logic Post-translational control RNA-based control Localization Measurement Input and Outputs (PoPS - Polymerase Per Second) Genetic stability Cross-lab consistency Operating Environment Load on the cell Environmental impact on performance Models

29. parts.mit.edu Sponsors National Science Foundation SynBERC CSBi GENEART Microsoft Research Biological Engineering - MIT

30. parts.mit.edu Teachers Workshops MIT May 26 th China June 16-17 Zurich June 27 th Hands-on training Future instructors welcome

31. parts.mit.edu How can I participate? Organize a team for 2008 Volunteer for the BioBrick parts program. Join the Awards committee Be a judge Have your lab join the Registry Contribute parts to the Registry Use BioBrick part standards Help raise money for teams and ambassadors in your area. Join iGEM committees (see the wiki) Join iGEM in Europe