1. Professor: Cheng-Hsien, Liu Student: Hao-Ran, Shih (9933533) Date: 2010/12/28 Philip N. Duncan, Transon V. Nguyen and Elliot E. Hui Department of Biomedical Engineering, University of California, Irvine, CA, USA

2. Outline ABSTRACT INTRODUCTION THEORY EXPERIMENTAL CONCLUSION

3. ABSTRACT 1.A precision pneumatic oscillator which provides timing signals for integrated microfluidic digital logic circuits 2.The design is based on the classical ring oscillator circuit and requires only a vacuum supply for power 3.Integrate pneumatic and fluidic circuits to create an autonomously driven peristaltic pump

4. INTRODUCTION 1.Microfluidic Large-Scale-Integration has been a highly successful technology for the automation of multiplexed chemical reactions 2.A control system built solely out of microfluidic components would be attractive because it could be manufactured in parallel with fluid-handling elements on a single chip 3.Lab-on-a-chip devices require timing to control fluid- handling elements such as peristaltic pumps must be driven by carefully coordinated waveforms

5. Device fabrication THEORY

6. Mathies technology IN OUT Vacuum Source Ground

7. Ring Oscillator Circuit 1.The system is inherently unstable and will thus oscillate indefinitely. (odd number ) 2.The frequency of oscillation should vary linearly with 1/(pneumatic resistance ), allowing tuning of the oscillator.

8. EXPERIMENTAL mask layout for a 3- inverter ring oscillator

10. photodiode detector Movement of the elastomeric membrane in the valve causes a deflection of the laser beam resulting in a change in measured intensity

11. Frequency Design other resistances in the circuit begin to dominate, such as the resistance of the lines connecting the 3 inverter stages saturate

12. changing the mask layout to minimize interconnect distances

13. The worst long-term drift was measured to be approximately 4% per hour. The timing of a 1-hour chemicalreaction should be accurate to within 3 minutes.

14. The use of an oscillating pneumatic circuit to drive the operation of a peristaltic pump Next page

16. CONCLUSION 1.The tuning of oscillator frequency through the variation of resistor sizes in the circuit, achieving a range of 1 Hz to 100 Hz. 2.No characterized oscillator stability,but establishing in devices that the short-term fluctuation and longterm drift is suitable for lab-on-a-chip applications. 3.The only external input required is a vacuum source, it may be possible to use such devices in limited-resource settings.

17. Thanks for your listening~