3. This includes but is not limited to:  Vicon  Switch on power strip located on wire rack  Should be turned on before software is opened  EMG  Delsys Trigno  Is switched on when power strip turned on  Force Plates  2 small boxes labeled Bertec  Green lights indicate on  Push the button on the right side of each Bertec box to execute a hardware re-zero

4.  Once Vicon Hardware is powered on, open Vicon Software found on desktop  Change all view types to RELIEF (highlighted in yellow)

5.  Calibration is most effective with two people  On right of screen, select the System Preparation under Tools  Choose 5-marker wand and L-Frame under the 2 pull down menus  Have one person stand by area of force plates with the calibration wand  The other person should hit start under Calibrate Cameras subheading.  Calibration Type should read Full Calibration  After “start” is clicked, the person with the wand should move the wand around the entire volume making sure to turn around so that all cameras can view the wand.  As a camera is completed, it will turn green. Process ends when all cameras are green in Vicon.  Once this is complete, wait for the system to finish calibration (System Preparation) (System) These values should be below 0.16. If not, redo the calibration before saving.

6.  Grab the T-wand and place it on the upper right corner of Plate #1 (plate on right when looking at them from the captains deck)  Click Start under the Set Volume Origin subheading as seen on left, and then hit Set Origin

7. 2. Check Camera Views:  Hide all reflective markers, including the wand used in camera calibration  Window  New Floating Workspace (Picture one), results in picture two  Under the 3D Perspective tab on top left, select “Camera” (Shown in picture two)  Select the cameras (one at a time or hold Control key to select all cameras)  To do this, expand the Cameras sub-heading under the “System” tab as shown in picture three.  Picture four shows results of selecting all seven cameras  If there are white dots in camera field boxes, this indicates the presence of reflective markers or objects that reflective components that the cameras are picking up. Move markers and/or reflective objects out of camera viewing area. (Picture One) (Picture Two) (Picture Three) (Picture Four)

9. General Information  Fabrifoam straps are arranged on wall by body region and by size in subject set-up area  The straps are used to secure extremity marker plates  Wrap straps tightly around body segments to prevent them from sliding down during the data collection process Upper Extremity  Left and Right mid-humerus  Left and Right mid-forearm Lower Extremity  Left and Right mid-thigh  Left and Right mid-shank Sacrum  Use black belt and secure it below the PSIS and ASIS

10.  Apply the extremity plates to the Velcro /elastic straps  Same concept for right side. Only difference in marker identification in Vicon is that labels start with “R” instead of “L”  Use this picture to help in labeling markers in Vicon

12. LFing Rfing Rfootlat Rfootmed Rtoe HeadRFront HeadLBack HeadRBack  Use surgical cap and place head cluster on top 4 markers on it. Above is a top view of hat markers.  Apply finger markers to left and right index fingers as seen in picture on left.  Use white sneakers for subjects’ feet. There are plates labeled right and left toe to use HeadLFront

13.  Use double-sided tape to attach reflective markers to the following: Upper Extremity:  Left and Right, anterior and posterior GH (Shoulder)  Left and Right, medial and lateral humeral epicondyles (Elbow)  Left and Right, radial and ulnar styloid processes (Wrist)  C7 vertebrae (not shown)  Offset to the left of the lumbar cluster at level of T12/L1 (not shown) Lower Extremity:  Left and Right Greater Trochanters (Hip)  Left and Right, medial and lateral femoral condyles (Knee)  Left and Right, medial and lateral malleoli (Ankle)

14.  Follow EMG Set-Up as outlined in EMG section of Lab Manual.  The RELIEF trial uses sensors:  1: ERS_R  2: EXO_R  3:RAB_R  4: INO_R  5:ERS_L  6:EXO_L  7:RAB_L  8: INO_L  13: DEL_R  14: DEL_L  Once all sensors are placed, turn them on.  Open the Trigno utility from the toolbar, click Start.  All sensors should show that they are paired indicated by the green light and the “paired” icon by each sensor.

15. Create the subject and session:  File  data management  Highlight appropriate study by double clicking it  Click New Patient as represented by the yellow marker  Type in the subject base file name  Click on the gray marker at the top of the screen labeled New Session  Under this heading type the base file name followed by S1  Repeat this process three more times, labeling the base file with S2, S3, S4 Load Subject:  In the left column on main screen, select the “subjects” tab (as compared to systems)  Select the button Create a Blank Subject  Enter the base file name of the subject  Right Click, select, Attach Model  Select, RELIEF (06-03-13) (Subject’s Tab) (Shortcut for data management)

16. Check All Analog Signals:  Make sure all sensors are paired by opening up the Trigno utility  Under the 3D perspective pull down menu, select “Graph” (picture one)  Under the system tab on left, select various EMG channels, load cells, fork sensors, etc. to make sure all equipment is working (picture two)  Apply manual force to subject to check activity of EMG channels  Picture three shows sampling of an EMG channel in the graph workspace (Picture One)(Picture Two)(Picture Three)

17. Collect Static Trial & Re-construct  Have the subject step into the space  Make sure you are in Live mode  Under the Tools, select Capture (directors clapboard)  Have the subject assume the T-pose  Instruct subject to look straight ahead, and flex shoulders to 80 degrees with thumbs pointing up  Make sure all sensors are visible, readjust sensors on the subject if they are not  Instruct subject to not move  Click Start and collect ~400 frames  Under the Subject Capture subheading, click start to record data, click stop after about 1-2 seconds of collection.  Open the Data Management window and double click on the previously captured trial  Click on the Gray Markers at the top of the screen under File, Edit, Window, Help

18. Labeling Markers:  On the right side of the main screen, select “Label/Edit” button. It looks like a tag.  Click on first marker name from list on right (PelLtSup)  Click on corresponding marker  Use previously labeled pictures to help correctly label markers  Vicon will cue you as to which marker you should label next, so you don’t have to click on the list on the right again  If you label a marker wrong, click Control-Z for edit undo function. Do that until you get rid of all mistakes.  After all markers are assigned, press the Escape button on the keyboard which will allow Vicon to connect the markers  When finished, click on save shortcut button  Remove all joint markers in the extremities for subsequent trials Helpful Reminders:  Joint marker names are based on anatomical position  During calibration trial, subject has shoulders flexed to 80 degrees with thumbs up  Shoulder back is more posterior  Shoulder front is more anterior  Elbow lat is higher  Elbow mid is lower  Wrist Rad is higher  Wrist ulna is lower

19.  Picture above shows all markers used for calibration trial. Blue markers are the joint markers that are removed after calibration. Includes bilateral wrist, elbow, shoulder, hip, knee, and ankle markers.  Picture above shows all markers used all subsequent trials. Notice how joint markers have been removed. This is accomplished by simply physically removing joint markers off subject after calibration trial completed.

20. Data Collection:  On the right side of the main screen, select the “capture” button. It looks like a director’s board.  Vicon must be in Live  Don’t start collecting data until model is attached. If the model is not attaching, have the subject assume the T-pose as used in the static calibration.  Under the Auto Capture Setup subheading; select “Arm” to arm the trigger  You can now use the handheld trigger to collect data.  Data collection duration and other specific settings may vary depending on specific protocol. (Arm) (Capture)