1. General Microbiology M122 TuesdayThursdayFriday Big 400 students lecture Biology/Microbiology majors (JR, SR) + nursing students (SO) GOAL: To gain some insight into the role of microbial communities in human health

2. In-class Out-of-class TuesdayThursday Monday Wednesday Friday Weekend LEARNING OBJECTIVE: To infer the relationship between human gut microbiome diversity, metabolism and environment Practice exam questions Reading guide + pre-reading quiz Clickers, think-pair-share, group discussion

3. Learning outcomeTLearning activity/Formative assessmentSum asses To describe how the gut microbiome is profiled and how diversity is measured Understand -Reading guide + Pre-reading quiz: sequencing technology and diversity. -Clicker question: select population with the highest diversity. -Think-pair-share: how we can determine taxonomic and functional “abundance” by sequencing”? board/virtual board. -Practice exam questions questions during discussion sections Multiple choice quiz/exam To show one example of how microorganism fermentation promotes food metabolism and absorption Analyze -Reading guide + Pre-reading quiz: microorganism fermentation in the gut -Group discussion: outline how digestible and non-digestible carbohydrates are metabolized and absorbed, showing cooperation between human and bacterial enzymes board/virtual board. -Clicker question + Think-pair-share: connect sequencing functional data with metabolic pathways. -Practice exam questions questions during discussion sections To interpret data describing taxonomic and functional diversity of the gut microbiome under different environmental conditions (diet, age, etc) Evaluate -Reading guide + Pre-reading quiz: 2-3 figures from Journal articles. Answer questions about hypothesis, what graph is showing, results and conclusion. Bring answers to class. -Group discussion: discuss figures based on the questions they have in reading guide -Clicker + Socratic discussion: questions about experimental design, what figure from paper is showing, results, conclusions -Practice exam questions questions during discussion sections LEARNING OBJECTIVE: To infer the relationship between human gut microbiome diversity, metabolism and environment

4. In all the cases shown below, the populations in the upper panel are more diverse than the ones shown in the lower panel A.TRUE B.FALSE

5. Turnbaugh et al. 2009. The Effect of Diet on the Human Gut Microbiome: A Metagenomic Analysis in Humanized Gnotobiotic Mice. Sci Transl Med 1, 6ra14 Microbiome plays some role in obesity -What is a LF/PP diet? -What is a Western diet? -What are humanized gnotobiotic mice? -How did they run this experiment? Explain the timeline (what is in X-axis?) -What are they measuring on Y-axis? -what is acting as control here?

6. Your research indicates that bacteria that belong to the phylum Firmicutes have high capability to ferment refined sugars and digest fats. Bacteroidetes on the other hand, are better and degrading complex plant carbohydrates. You decide to run an experiment with humanized mice similar to the one we discussed in class: humanized mice growing in a Low fat/High plant carbohydrate diet are switched to a Western diet or kept on a Low fat/High plant carbohydrate diet as control. Based on this, you predict: A. Bacteroidetes will dramatically increase in the gut of control mice kept in a Low fat/High plant carbohydrate diet B. Firmicutes will increase in the gut of humanized mice switched to a Western diet Bacteroidetes will increase in the gut of humanized mice switched to a Western diet C. a and b are correct D. b and c are correct

7. Our Self Portrait: the Human Microbiome Reflection of one’s self in dialogue with its host Oil on canvas Two panels, 16 x 16 in by Joana Ricou, 2011 They are us, too

8. Our Courses Self Portrait Reflection of one’s self in dialogue with its course Oil on canvas Two panels, 16 x 16 in by Joana Ricou, 2011 Formative assessment should be there, too