Download presentation
Presentation is loading. Please wait.
Published byMartin Hodge Modified over 9 years ago
1
Enzyme Kinetics and Catalysis II 3/24/2003
2
Kinetics of Enzymes Enzymes follow zero order kinetics when substrate concentrations are high. Zero order means there is no increase in the rate of the reaction when more substrate is added. Given the following breakdown of sucrose to glucose and fructose Sucrose + H 2 0 Glucose + Fructose
3
E = Enzyme S = Substrate P = Product ES = Enzyme-Substrate complex k 1 rate constant for the forward reaction k -1 = rate constant for the breakdown of the ES to substrate k 2 = rate constant for the formation of the products
4
When the substrate concentration becomes large enough to force the equilibrium to form completely all ES the second step in the reaction becomes rate limiting because no more ES can be made and the enzyme-substrate complex is at its maximum value. [ES] is the difference between the rates of ES formation minus the rates of its disappearance. 1
5
Assumption of equilibrium k -1 >>k 2 the formation of product is so much slower than the formation of the ES complex. That we can assume: K s is the dissociation constant for the ES complex.
6
Assumption of steady state Transient phase where in the course of a reaction the concentration of ES does not change 2
7
3 Combining 1 + 2 + 3 rearranging Divide by k 1 and solve for [ES]Where
8
v o is the initial velocity when the reaction is just starting out. And is the maximum velocity The Michaelis - Menten equation
9
The K m is the substrate concentration where v o equals one-half V max
10
The K M widely varies among different enzymes The K M can be expressed as: As Ks decreases, the affinity for the substrate increases. The K M can be a measure for substrate affinity if k 2 <k -1
11
There are a wide range of K M, Vmax, and efficiency seen in enzymes But how do we analyze kinetic data?
12
The double reciprocal plot
14
Lineweaver-Burk plot: slope = K M /Vmax, 1/v o intercept is equal to 1/Vmax the extrapolated x intercept is equal to -1/K M For small errors in at low [S] leads to large errors in 1/v o k cat is how many reactions an enzyme can catalyze per second The turnover number
15
For Michaelis -Menton kinetics k 2 = k cat When [S] << K M very little ES is formed and [E] = [E] T and K cat /K M is a measure of catalytic efficiency
16
What is catalytic perfection? When k 2 >>k -1 or the ratiois maximum Then Or when every substrate that hits the enzyme causes a reaction to take place. This is catalytic perfection. Diffusion-controlled limit- diffusion rate of a substrate is in the range of 10 8 to 10 9 M -1 s -1. An enzyme lowers the transition state so there is no activation energy and the catalyzed rate is as fast as molecules collide.
17
Reaction Mechanisms A: Sequential Reactions All substrates must combine with enzyme before reaction can occur
18
Bisubstrate reactions
19
Random Bisubstrate Reactions
20
Ping-Pong Reactions Group transfer reactions One or more products released before all substrates added
21
Kinetic data cannot unambiguously establish a reaction mechanism. Although a phenomenological description can be obtained the nature of the reaction intermediates remain indeterminate and other independent measurements are needed.
22
Inhibition kinetics There are three types of inhibition kinetics competitive, mixed and uncompetitive. Competitive- Where the inhibitor competes with the substrate.
23
Competitive Inhibition
26
HIV protease inhibitors
27
Competitive Inhibition: Lineweaver-Burke Plot
28
Uncompetitive Inhibition
29
Uncompetitive Inhibition: Lineweaver-Burke Plot
30
Mixed inhibition
32
Mixed inhibition is when the inhibitor binds to the enzyme at a location distinct from the substrate binding site. The binding of the inhibitor will either alter the K M or V max or both.
34
The effect of pH on kinetic parameters
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.