1. Effective Date: 04/01/2013 KENTUCKY WATERSHED WATCH RECERTIFICATION – PHASE 1

2. Phase 1 Phase 1 Field Chemistry AssessmentField Chemistry Assessment Grab Sample CollectionGrab Sample Collection Training and Certification Workshops Phase 2 Phase 2 Biological AssessmentBiological Assessment Habitat AssessmentHabitat Assessment

3. Phase 1 Watershed Watch Recertification Goals for this module Refresh your skillsRefresh your skills Highlight changes to sampling proceduresHighlight changes to sampling procedures Distribute NEW flip booksDistribute NEW flip books Check out any replacement equipment you needCheck out any replacement equipment you need Complete essential recertification paperworkComplete essential recertification paperwork

4. Active Watershed Watch Sampling Sites

5. How we’re set up: Basin Steering Committee or Board of Directors Watershed Watch In Kentucky Board of Directors Sampling Event Coordinator Lab Manager Science Advisor(s) Area coordinator Area coordinator Area coordinator Watershed Watch Trained Sampler

6. Goal: Get people into the real world to see first hand the condition of their streams. hand the condition of their streams.

7. Watershed Watch provides you the tools and information you need to educate and inform YouFamilyFriendsNeighborsCommunity Local Leaders Resource Management Agencies

8. How you can use your information Personal decision makingPersonal decision making Local actionLocal action Grant applicationsGrant applications Watershed planningWatershed planning Assessment screeningAssessment screening Enforcement effortsEnforcement efforts Scientific researchScientific research Data usage depends on the quality control applied…

9. Standard Operating Procedures (SOP) Training workshops for certificationTraining workshops for certification Standard methods of collectionStandard methods of collection Standard methods of analysisStandard methods of analysis Full chain of custody and/or formsFull chain of custody and/or forms Careful recordkeeping!Careful recordkeeping! For good Quality Assurance of results, we require:

10. Standard Operating Procedures (SOP) To ensure standard methods of collection, always use flip cards when sampling:

11. This presentation is based on the “Kentucky Watershed Watch Field Chemistry Sample Collection Standard Operating Procedure WWSOP01000” and is available from wwky.org SOP Statement

12. Standard Operating Procedures (SOP) NEW Flip Book Field Chemistry

13. You will receive materials from program coordinators or by mail You will receive materials from program coordinators or by mail Read instructions upon receipt! (Critical info on sample times and delivery included) Read instructions upon receipt! (Critical info on sample times and delivery included) Inspect all sample and preservative containers upon receipt for damage or breakage Inspect all sample and preservative containers upon receipt for damage or breakage You will receive for sampling: InstructionsInstructions Transport informationTransport information Chain of Custody RecordsChain of Custody Records Gloves (disposable)Gloves (disposable) Sample containersSample containers Preservative (if required)Preservative (if required) Pre-sampling Information

14. During high flow, sampling should be postponed until baseline conditions exist – do not sample in any unsafe conditions Waders and specialized wading boots can be utilized Use extreme caution when wading in streams above the knee Samplers should exhibit caution in and around streams to reduce the threat of a falling injury Secure permission from landowner Be aware of increased risk for frostbite or hypothermia in cold temperatures Do not stick hands or feet under large objects. Exercise caution when handling aquatic life. Some organisms pinch, bite, etc.Do not stick hands or feet under large objects. Exercise caution when handling aquatic life. Some organisms pinch, bite, etc. All water bodies have the potential to be contaminatedAll water bodies have the potential to be contaminated Notify others of your whereabouts and carry a cell phoneNotify others of your whereabouts and carry a cell phone Use caution and be aware of your surroundings and locationUse caution and be aware of your surroundings and location Be Safe!

15. Equipment You will need to purchase a gallon of distilled water for use during sampling events You will need to purchase a gallon of distilled water for use during sampling events All field chemistry sample containers must be rinsed with distilled water after each test All field chemistry sample containers must be rinsed with distilled water after each test

16. Equipment Use your Waste Container to Use your Waste Container to dispose of all used chemicals dispose of all used chemicals After rinsing field chemistry After rinsing field chemistry sample containers, dispose of sample containers, dispose of rinse water in Waste Container rinse water in Waste Container After sampling event, flush After sampling event, flush contents of Waste Container contents of Waste Container into a system that goes to a into a system that goes to a wastewater treatment plant wastewater treatment plant

17. Store in a cool, dry place (climate controlled) Store in a cool, dry place (climate controlled) NOT a hot car or freezing garage! NOT a hot car or freezing garage! Store out of reach of children and pets Store out of reach of children and pets In addition: Chemicals should be replaced every three years at Chemicals should be replaced every three years at your recertification training your recertification training Kits have enough chemical for 50 tests! Kits have enough chemical for 50 tests! (use them all you want!) (use them all you want!) Should you need replacement chemicals or Should you need replacement chemicals or glassware before then, let us know glassware before then, let us know Equipment

18. There are hazardous chemicals in these kits! There are hazardous chemicals in these kits! Dissolved Oxygen (DO) test uses a strong acid and alkali (like bleach) Dissolved Oxygen (DO) test uses a strong acid and alkali (like bleach) pH Test chemical is flammable pH Test chemical is flammable Read Material Safety Data Sheets (MSDS) for Read Material Safety Data Sheets (MSDS) for further information on chemical safety further information on chemical safety Dispose of used chemicals in plastic waste Dispose of used chemicals in plastic waste container at stream and then flush into municipal container at stream and then flush into municipal wastewater treatment system (no septic systems) wastewater treatment system (no septic systems) Trade in old chemicals for proper disposal when Trade in old chemicals for proper disposal when you receive replacement chemicals you receive replacement chemicals Equipment Chemical Safety

19. Proper attire for performing tests Gloves (disposable)Gloves (disposable) Eye protectionEye protection Equipment Chemical Safety

20. What to do if you get a chemical on you For emergency first aid in the field, flush For emergency first aid in the field, flush affected area with large amounts of water affected area with large amounts of water and seek medical attention if necessary and seek medical attention if necessary Follow the first aid procedures found on Follow the first aid procedures found on the Material Safety Data Sheets (MSDS) the Material Safety Data Sheets (MSDS) Equipment Chemical Safety

21. Field Observations and Water Chemistry Assessment Enter stream at a downstream location Enter stream at a downstream location Walk upstream to avoid disturbing bottom Walk upstream to avoid disturbing bottom Perform your observations and tests just below your Perform your observations and tests just below your grab sample location grab sample location Sampling Procedure

22. 0 = Dry 1 = Ponded 2 = Low 3 = Normal 4 = Bank Full 5 = FLOOD! Field Observations Flow Rate 0-5 Point Scale Do not sample during these flow conditions

23. 48 Hour Rainfall Use Weather Channel, local paper or rain gauge OR Use any of these websites www.cocorahs.org/state.aspx?state=ky wwwagwx.ca.uky.edu/cgi-bin/ky_clim_data_www.pl water.weather.gov/ www.kymesonet.org/ Field Observations To determine 48 hour rainfall in inches

24. Site coordinates and Photos GPS (decimal degrees – 5 decimal places) GPS (decimal degrees – 5 decimal places) OR Digital map (KGS WW Site - http://kgs.uky.edu/kgsmap/krww/viewer.asp http://kgs.uky.edu/kgsmap/krww/viewer.asp or Google Earth) Take photos upstream and downstream of sampling point – with recognizable landmarks; should be taken annually during Summer sampling event Take photos upstream and downstream of sampling point – with recognizable landmarks; should be taken annually during Summer sampling event Label photos: “Site#upstream” and “Site#downstream” Label photos: “Site#upstream” and “Site#downstream” Submit information to Water Watch office Email: OllieTheOtter@ky.gov Submit information to Water Watch office Email: OllieTheOtter@ky.gov Phone: 1-800-928-0045 Additional Field Observations

25. Put on gloves Put on gloves Collect water just below your grab sample location Collect water just below your grab sample location Rinse bottle, tube or cup and any associated caps or Rinse bottle, tube or cup and any associated caps or lids with stream water three times (fill container ¼ full of lids with stream water three times (fill container ¼ full of stream water, cap, shake, and discard downstream) stream water, cap, shake, and discard downstream) Point mouth of bottle, tube or cup directly at the surface Point mouth of bottle, tube or cup directly at the surface of the water, submerge entire container to mid-depth of of the water, submerge entire container to mid-depth of stream, scoop upstream and fill to appropriate level stream, scoop upstream and fill to appropriate level For Field Chemistry tests: pH pH Dissolved Oxygen Dissolved Oxygen Conductivity Conductivity (does not include Temperature) Field Measurements

26. Conductivity Equipment Field Measurements Meter Calibration Must be calibrated for Must be calibrated for each sampling event each sampling event Calibrate to 720 µS Calibrate to 720 µS (standard solution is (standard solution is 718 µS) 718 µS) New Meter Old Meter

27. Watershed Watch Chain of Custody Record Conductivity Equipment Field Measurements Meter Calibration Record meter calibration Record meter calibration date on COC date on COC Must be calibrated Must be calibrated within 24 hours prior within 24 hours prior to sampling event to sampling event

28. Conductivity Equipment – New Meter Field Measurements New Meter Calibration Display Display Hold/enter Hold/enter Calibrate Calibrate Power Power Calibration Standard Calibration Standard

29. Conductivity Equipment Field Measurements

30. Conductivity Equipment – Old Meter Field Measurements Old Meter Calibration Display Display Hold Hold On/off On/off Calibration Standard Calibration Standard

31. Conductivity Equipment Field Measurements Decrease Increase

32. Conductivity Equipment Field Measurements Calibration (Old Meter): Twist open lid on wider end (battery compartment) Place narrower end (electrodes) in calibration standard Press ON/OFF button and wait for display to stabilize When viewed from top, hold meter with white buttons on left Press the Increase (top) or Decrease (bottom) button until display reads 720 µS When display flashes 3 times, then shows ENT, the meter is calibrated Replace lid

33. Water Conductivity Test Procedure Field Measurements Fill sampling container halfway with stream water Fill sampling container halfway with stream water Press Power (ON/OFF) button and allow to stabilize Press Power (ON/OFF) button and allow to stabilize to 0 in air to 0 in air Place meter in sample, Place meter in sample, stir once, and wait for stir once, and wait for reading to stabilize reading to stabilize Record result in µS Record result in µS Rinse container and Rinse container and narrower end of narrower end of meter (electrodes) meter (electrodes) with distilled water with distilled water

34. Field Measurements Remember to rinse the narrower end of meter (electrodes) and sampling container with distilled water!

35. Water Temperature Test Procedures Field Measurements Place thermometer at mid-depth of stream in location with constantly flowing water Place thermometer at mid-depth of stream in location with constantly flowing water Leave thermometer Leave thermometer there until reading stabilizes Record temperature in Record temperature in degrees Celsius (°C)

36. This presentation is based on the “Kentucky Watershed Watch Stream Grab Sample Collection Standard Operating Procedure WWSOP02000” and is available from wwky.org Grab Sample SOP

37. Standard Operating Procedures (SOP) NEW Flip Book Grab Sample

38. Watershed Watch Chain of Custody Record The sampling containers are pre-marked with a unique Site Number The Site Number should match the number on the “Chain of Custody Record” Pre-sampling Information

39. I received a bottle with a site number I don’t recognize! Check the documents in your packet, you may have been randomly assigned to collect a blank or a duplicate Pre-sampling information

40. Blank - A sample bottle filled while at the stream site with distilled water Serves as a check for possible contamination during sample handling or processing at the labServes as a check for possible contamination during sample handling or processing at the lab DO NOT indicate that the sample is a blank – the lab should not knowDO NOT indicate that the sample is a blank – the lab should not know Pre-sampling Information

41. Duplicate - A sample bottle filled with stream water at your site at the same time as your regular sample Serves as a check for variability among samples that should yield approximately the same lab resultsServes as a check for variability among samples that should yield approximately the same lab results DO NOT indicate that the sample is a duplicate – the lab should not knowDO NOT indicate that the sample is a duplicate – the lab should not know Pre-sampling Information

42. Sample collection location Collection Procedures Sample in an area of the stream where the flow is concentrated and where you can completely submerge the bottle without touching the bottom NOTE: It is important that you collect your sample at the same location each time

43. Concentrated Flow Location Collection Procedures

44. Grab Sample Collection Enter stream at a downstream location Enter stream at a downstream location Walk upstream to avoid disturbing bottom Walk upstream to avoid disturbing bottom Collect sample at the same location each time in Collect sample at the same location each time in the middle of your concentrated flow location the middle of your concentrated flow location and at mid-depth of stream and at mid-depth of stream Collection Procedures

45. Put on disposable gloves Put on disposable gloves DO NOT RINSE BOTTLE DO NOT RINSE BOTTLE Point mouth of bottle directly over surface of water, dip Point mouth of bottle directly over surface of water, dip and scoop bottle upstream beneath water surface, and and scoop bottle upstream beneath water surface, and through water column in one swift motion to fill above through water column in one swift motion to fill above line marked on bottle line marked on bottle If preservative is in bottle, take caution to angle mouth so If preservative is in bottle, take caution to angle mouth so as not to spill any while collecting sample as not to spill any while collecting sample Cap container, leaving at least a small airspace at top to Cap container, leaving at least a small airspace at top to allow lab mixing allow lab mixing Place in wet ice immediately Place in wet ice immediately ForE. coli For E. coli grab sample collection: Collection Procedures

46. Put on disposable gloves Put on disposable gloves Rinse bottle with stream water three times Rinse bottle with stream water three times (fill bottle ¼ full of stream water, cap, shake, and discard downstream) Point mouth of bottle upstream (against the flow), Point mouth of bottle upstream (against the flow), submerge entire bottle to mid-depth of stream, submerge entire bottle to mid-depth of stream, scoop and fill scoop and fill Rinse cap in stream and cap bottle Rinse cap in stream and cap bottle Add preservative (if necessary) and place in wet ice Add preservative (if necessary) and place in wet ice Collection Procedures For grab sample collection other thanE. coli: For grab sample collection other than E. coli:

47. Samples must be kept immersed in wet ice Samples must be kept immersed in wet ice (but not frozen) until received by laboratory Some samples may require other preservatives Some samples may require other preservatives (check your sample event packet) Preservation

48. Preservation Sample Holding Time: length of time a preserved sample can be stored length of time a preserved sample can be stored after collection and before it is analyzed without after collection and before it is analyzed without significantly affecting the results significantly affecting the results from the minute you collect a sample, the from the minute you collect a sample, the clock starts ticking on the holding time clock starts ticking on the holding time varies for different types of analysis varies for different types of analysis established by the US Environmental established by the US Environmental Protection Agency Protection Agency

49. E. coli = 6 hours maximum Other than E. coli = Follow holding time instructions received in your sampling event packet Preservation Sample Holding Times

50. Watershed Watch Chain of Custody Record Date and Time of sample collectionDate and Time of sample collection Field Observations and MeasurementsField Observations and Measurements Date and time of sampleDate and time of sample Relinquished/Received Relinquished/Received must be identical Recordkeeping Volunteer entries on the Chain of Custody Cross through any mistakes and initial – do not erase

51. Phase 1 (Program Logistics, Field Chemistry Phase 1 (Program Logistics, Field Chemistry and Grab Sample) Training: and Grab Sample) Training: Your sampling and field testing skills are considered Your sampling and field testing skills are considered current for three years after completing current for three years after completing WWKY-approved training WWKY-approved training Training updates must be completed at least every Training updates must be completed at least every three years three years In addition, chemicals must be replaced every three In addition, chemicals must be replaced every three years for your field test results to be accurate. These years for your field test results to be accurate. These chemicals should be replaced at your training update. chemicals should be replaced at your training update. Recertification

52. IMPORTANT: If you observe an outfall, pipe, or anything that might indicate illegal pollution of a waterway, contact Kentucky Division of Water to report complaints or concerns at 502-564-3410 during business hours. For environmental emergencies such as spills of gas, oil or other substances, contact the Environmental Response Team at 502-564-2380 or 1-800-928-2380. Environmental Safety

53. Go to your basin’s Watershed Watch Webpage through the “Quick Links” at: How do I find out more about my basin? wwky.org

54. Phase 2 – Biological and Habitat Assessment Habitat Assessment Stream and Watershed Investigation Stream and Watershed Investigation Methods Methods Classroom and Streamside Instruction Classroom and Streamside Instruction Biological Assessment Benthic Macroinvertebrate Collection Benthic Macroinvertebrate Collection and Identification Methods and Identification Methods Classroom and Streamside Classroom and Streamside Instruction Instruction

55. Streams are constantly changing GRAB SAMPLE & FIELD CHEMISTRY Instantaneous waterInstantaneous water quality quality Quick processQuick process Specific pollutantSpecific pollutant analysis analysis BIOLOGICAL & HABITAT ASSESSMENT Water quality overWater quality over time time Takes more timeTakes more time Collective pollutionCollective pollution indication indicationVS.

56. Phase 2 (Biological and Habitat Assessment) Phase 2 (Biological and Habitat Assessment) Training: Training: Your sampling and assessment skills are considered Your sampling and assessment skills are considered current after completing WWKY-approved training current after completing WWKY-approved training You will be notified that training updates are required You will be notified that training updates are required if modifications are made to WWKY methods if modifications are made to WWKY methods Recertification

57. If you have any questions after today’s workshop, please contact your “Area Coordinator” or the KY Water Watch Office at 1-800-928-0045 Questions?

58. ParticipantAgreementRecords: Accurate contact Accurate contact information information Equipment loaned Equipment loaned Site(s) selected Site(s) selected Training received Training receivedParticipantAgreementRecords: Accurate contact Accurate contact information information Equipment loaned Equipment loaned Site(s) selected Site(s) selected Training received Training received

59. Fill out Participant Agreement Form, sign and turn into trainer Fill out Participant Agreement Form, sign and turn into trainer