1. Virginia iGEM Workshop #4 High School Education Series

2. Workshop Plan Virginia iGEM Workshop #4 High School Education Series Lesson: PCR, Gel Electrophoresis, & Transformation Activity: Make Your Own Primers

3. Virginia iGEM Workshop #4 High School Education Series Polymerase Chain Reaction

4. Virginia iGEM Workshop #4 High School Education Series Polymerase Chain Reaction 3D PCR Animation: http://www.dnalc.org/view/154 75-The-cycles-of-the- polymerase-chain-reaction-PCR- 3D-animation-with-no- audio.html

5. Virginia iGEM Workshop #4 High School Education Series Primer Design

6. Virginia iGEM Workshop #4 High School Education Series Gel Electrophoresis

7. Virginia iGEM Workshop #4 High School Education Series Sequencing Sanger Sequencing Explanation: http://www.dnalc.org/resources/3d/29-sanger-sequencing.html

8. Virginia iGEM Workshop #4 High School Education Series Transformation

9. Activity: Make Your Own Primers Virginia iGEM Workshop #3 High School Education Series DON’T FORGET! 18-22 base pairs 50-60% GC Content Wallace Rule: 4 degrees per C or G, 2 degrees per A or T  Melting temperature of the primer. Subtract two degrees for annealing temperature. Melting temperature 55-60 degrees 3’ Nucleotide = C or G NCBI Primer Blast: http://www.ncbi.nlm.nih.gov/tools/primer-blast/ Primer3: http://frodo.wi.mit.edu/

10. Any Questions? Virginia iGEM Workshop #4 High School Education Series