2. Nature Nanotechnology, January 2011

3. Part I: Understanding Tissue

4. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011.

11. Part II: Fabricating the ECM

12. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011.

13. Barnes, C.P. et al. Nanofiber technology: Designing the next generation of tissue engineering scaffolds. Adv. Drug. Deliv. Rev. 2007

14. Zhang, S. Fabrication of novel biomaterials through molecular self-assembly. Nature Biotechnol. 2003.

19. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011.

20. Re’em, T. The effect of immobilized RGD peptide in macroporous alginate scaffolds on TGFbeta1-induced chondrogenesis of human mesenchymal stem cells. Biomaterials. 2010. Matrix modified with adhesion proteins Unmodified scaffold Spreading and attaching to matrix Only cell-cell adhesion

21. Freeman, I. The effect of sulfation of alginate hydrogels on the specific binding and controlled release of heparin-binding proteins. Biomaterials. 2008. bFGF bound to scaffold bFGF adsorbed to scaffold bFGF absent

22. Freeman, I. The effect of sulfation of alginate hydrogels on the specific binding and controlled release of heparin-binding proteins. Biomaterials. 2008. bFGF bound to scaffold bFGF adsorbed to scaffold bFGF absent

23. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Teixeira, AI. Epithelial contact guidance on well-defined micro- and nanostructured substrates. J. Cell. Sci. 2003. Flat topography Grooved topography

24. Part III: Designing a Scaffold

25. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Kim, DH. Nanoscale cues regulate the structure and function of macroscopic cardiac tissue constructs. PNAS. 2010.

26. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Feng, ZQ. The effect of nanofibrous galactosylated chitosan scaffolds on the formation of rat primary hepatocyte aggregates and the maintenance of liver function. Biomaterials. 2009.

27. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Roohani-Esfahani, SI. The influence hydoxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coarted with hydroxyapatite-PCL composites. Biomaterials. 2010.

28. Part IV: Enhancing the Engineering Matrix

29. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Wang, S. F., Shen, L., Zhang, W. D. & Tong, Y. J. Preparation and mechanical properties of chitosan/carbon nanotubes composites. Biomacromolecules. 2005 Gui, X. et al. Soft, highly conductive nanotube sponges and composites with controlled compressibility.ACS Nano. 2010.

30. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Wu, S. L. et al. A biomimetic hierarchical scaffold: natural growth of nanotitanates on three-dimensional microporous Ti-based metals. Nano Lett. 2008

31. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Zhang, SF. Nanoparticulate systems for growth factor delivery. Pharm. 2009.

32. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Fan, D. Subcellular-resolution delivery of a cytokine through precisely manipulated nanowires. Nature Nanotechnology. 2010.

33. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Souza, G. Three-dimensional tissue culture based on magnetic cell levitation. Nature Nanotechnology. 2010.

34. Part V: Monitoring Tissue Development

35. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Tian, B. Three dimensional, flexible nanoscale field-effect transistors as localized bioprobes. Science. 2010.

36. Dvir, Tal, et al. Nanotechnological strategies for engineering complex tissues. Nature Nanotechnology. 2011. Heller, DA. Multimodal optical sensing and analyte specificity using single-walled carbon nanotubes. Nature Nanotech. 2009.

37. Nature Nanotechnology, January 2011

38. The End