1. laboratory automation experimental setups

2. who are paa ?

3. who are process analysis & automation ? was section at British Petroleum incorporated in 1992 software supplier of OVERLORD suite offer systems integration services independent automation supplier experienced in multi-vendor platforms – PE/GE/Thermo/Caliper/Tecan/Beckman/Hamilton – Drivers for 75+ manufacturers, 270+ instruments unique software solutions – Tried and tested, over 400 sold worldwide

4. areas of interest acoustic emission sonic spectrometer quality software, 21CFR laboratory automation

5. laboratory automation experience 1986 1988 1995 19982004

6. why do we automate ? speed reliability extend instrument operating hours cost effective analysis data and sample tracking User satisfaction

7. why do we automate ?

8. analyze sampleprepare sample analyze sample prepare sample Laboratory automation - the process collect sample report results

9. an example Automated plate reader http://www.paa.co.uk/ftp/labauto/videos/reader_bmg_lo.wmv

10. what automation to offer ?

11. what automation to offer listen to customer – some what ‘off the shelf’ – some what full flexibility – some want simple – some have no idea what they require and do you … – sell them what they ask for – sell them what they need

12. what is an integrator ?

13. instruments are BRICKS software is the CEMENT integrator puts it together like a BUILDER hardware softwareintegration system

14. what can an integrator offer ? what happens before the plate is read ? – automation expertise – customer specific user interfaces what happens after the plate is read – data handling expertise – post run data manipulation – database interaction installation expertise – highly skilled installation team – installation engineers are programmers as well

15. how do we automate ? 1. specification – what, where, when, why & how ? – “by next month”, “flexible” – URS, FDS, FAT, SAT – data handling requirements – system throughput 2. hardware list 3. integration software

16. 1. specification User Requirement Specification (URS) – what do you want ? Functional Design Specification (FDS) – this what we are going to deliver Factory Acceptance Test (FAT) – check it works before it ships Site Acceptance Test (SAT) – check it still works after shipping

17. 2. hardware list Plate manipulatorHigh content plate reader Peripherals

18. hardware list – robots

19. automate.it SCARA hardware list – robots 2 PE PlateStakMitsubishi

20. hardware list – stackers PE PlateStakThermo RapidStak

21. hardware list - storage Incubator Storage

22. hardware list – liquid handling Reagent dispensing Flexdrop Liquid Handling PlateWash

23. hardware list – other peripherals Barcode reader Plate reader

24. control & scheduling software

25. control and scheduling software Two types – STATIC, pre-emptive, time-driven – DYNAMIC, real-time, event-driven

26. control and scheduling software STATIC hit the GO button know exactly what will happen at any time in the process DYNAMIC hit the GO button Follow a set process

27. control software - summary real-time scheduling – OVERLORD Workstation – event driven time-resolved scheduling – OVERLORD Scheduler – time driven Either

28. what is OVERLORD ? explanation of the difference between scheduler types with our two products

29. what is Overlord? suite of automation software by paa product range is 10 years old support for more than 270 instruments two main products – DYNAMIC: Overlord2 – STATIC: OVERLORD Scheduler

30. common features support for over 270 instruments data handling facilities utilizes original instrument software can integrate with most databases – SQL server, oracle, access 21 CFR part 11 compliant full system tracking error notification error recovery

31. which product ? AdvantagesDisadvantages Very Flexible True Dynamic Scheduling Takes longer to setup Very easy to use Obeys critical timings Schedules fixed processes Cannot make decisions Overlord2 OVERLORD Scheduler

32. Overlord2

33. dynamic/real-time/event icon based drag and drop set-up flow chart interface very flexible dynamic scheduling – decisions can be made in real-time

34. Overlord2 - Customizable user GUI & runtime module separable can use different GUI, Excel could be used different run engines can be run processes the flow using Overlord variables overlord instrument drivers developed quickly all components can be modified master or slave all errors handled with different options.NET framework, API will be utilized flexible GUI makes writing assays easier

35. which product ? AdvantagesDisadvantages Very Flexible True Dynamic Scheduling Takes longer to setup Very easy to use Obeys critical timings Schedules fixed processes Cannot make decisions Overlord2 OVERLORD Scheduler

37. static/time driven icon based drag and drop Gantt chart interface optimise and interleave different assays e ach assay run with exact incubation timings very easy to create an assay add new assays when the system is running

38. example systems

39. no stacker or laboratory robot PerkinElmer JANUS system http://www.paa.co.uk/ftp/labauto/videos/elisa_jpm_lo.wmv

40. stacker only automated TTP Mosquito system http://www.paa.co.uk/ftp/labauto/videos/nano_mosquito_lo.wmv automated PerkinElmer PlateStak system http://www.paa.co.uk/ftp/labauto/videos/elisa_pe_lo.wmv

41. laboratory robot systems automated Innovadyne Nanodrop system http://www.paa.co.uk/ftp/labauto/videos/nano_innovadyne_lo.wmv automated GE Healthcare IN Cell 1000 system http://www.paa.co.uk/ftp/labauto/videos/hcs_1000_lo.wmv

42. factory robot systems automated dilute & seal system http://www.paa.co.uk/ftp/labauto/videos/mitsubishi_dilute_seal_lo. wmv automated cell system http://www.paa.co.uk/ftp/labauto/videos/ppw_lo.wmv

43. tracked robot system BSE workcell http://www.paa.co.uk/ftp/labauto/videos/milk_lo.wmv DNA workcell http://www.paa.co.uk/ftp/labauto/videos/dna_fss_lo.wmv

44. conclusion laboratory automation has a number of key stages choice – hardware – software dynamic scheduler – integration project management – URS, FDS, FAT & SAT flexible features make the system what was required not what was asked for