1. Skeletal Muscle Key words:

2. Overview of the Muscle Fiber Structure Muscle fibers (cells) are composed of myofibrils Myofibrils are composed of myofilaments Myofilaments are composed of a thin filament (contains actin) and thick filament (contains myosin) The plasma membrane of the muscle cell is called the Sarcolemma

3. Sarcomere – One of the segments into which a myofibril is divided, it is the basic unit of contraction of skeletal muscle Z lines (Z discs) – the boundaries of each sarcomere Thin (actin) filaments – extend from Z disc toward the center of the sarcomere Thick (myosin) filaments – located in the center of the sarcomere; overlap inner ends of the thin filaments Sarcomere

4. A bands – full length of the thick filament; includes inner end of thin filaments (thus, there is overlap of thin and thick filaments) H zone – center part of A band where no thin filaments occur (thus, there is no overlap of thin and thick filaments) I band – region with only thin filaments (thus, there is no overlap of thin and thick filaments) M line – the center of sarcomere

5. Thin filament The thin filaments are composed of the contractile protein called actin plus some regulatory proteins: Troponin and Tropomyosin

6. Thick Filaments The thick filaments are made of bundled molecules of the protein myosin, which contains an ATPase region that uses ATP to generate power for muscle contraction

7. Muscle Contraction In a relaxed muscle cell, the regulatory protein Tropomyosin prevent myosin binding

8. Muscle Contraction Upon stimulation, calcium is released from internal stores and binds to the regulatory protein troponin on the actin filaments This causes the regulatory proteins to change their shape and position, thus allowing the myosin heads to bind to actin

9. Muscle Contraction The physical attachment of myosin to actin causes the myosin heads to snap toward the center of the sarcomere Since the actin and myosin are still firmly bound to each other when this happens, the thin filaments are slightly pulled toward the center of the sarcomere This is called the sliding filament theory

10. The nerve stimulus Skeletal muscle cells must be stimulated by nerve impulses to contract One neuron and all the skeletal muscle cells it stimulates are a motor unit

11. When a nerve cell approaches the muscle it will stimulate, it branches off, forming junctions with the sarcolemma of different muscle cells. These junctions are called Neuromuscular Junctions Although the nerve endings and the muscle cells are very close, they never touch. The Gap between them is called the synaptic cleft Neuromuscular Junctions

12. Activity at the Neuromuscular Junction 1.When the nerve impulse reaches the junction, a neurotransmitter is released. The neurotransmitter specific to skeletal muscles is called acetylcholine 2.Acetylcholine (ACh) diffuses across the synaptic cleft and attaches to receptors on the sarcolemma 3.If there is enough ACh bound to the sarcolemma, the sarcolemma becomes temporarily permeable to sodium ions (Na + ); 4.Sodium ions rush into the muscle cell

13. 5.The inside of the muscle cell becomes positively charged, because it has all the Na + ions in it 6.The balance of charge is disrupted, and the electrical behavior of the sarcolemma is changed 7.An electrical current is formed, called an action potential 8.The action potential travels over the entire sarcolemma – the result is contraction of the muscle Activity at the Neuromuscular Junction