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Na+ Negatively charged groups (red) Na+ Positively charged counterions Rinse in buffer, add ionic compounds. Positive charges (triangles) stick.

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Presentation on theme: "Na+ Negatively charged groups (red) Na+ Positively charged counterions Rinse in buffer, add ionic compounds. Positive charges (triangles) stick."— Presentation transcript:

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6 Na+ Negatively charged groups (red) Na+ Positively charged counterions Rinse in buffer, add ionic compounds. Positive charges (triangles) stick. Negatives (squares) and neutrals (pentagons) do not stick Na+ Sodium ions replaced by positively charged ions Rinse Cation Exchange Chromatography

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8 From Wikipedia Commons, Author=Jeffrey M. Vinocur http://en.wikipedia.org/wiki/ File:Gel_electrophoresis_app aratus.JPG

9 From Wikipedia Commons. Author = Dr d12Dr d12 http://en.wikipedia.org/wiki/File:D NAgel4wiki.png

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