1. HIGH PERFORMANCE THIN LAYER CHROMATOGARPHY(HPTLC)BY
M.PAUL RICHARDS
MPAQA

2. WHAT IS PLANAR /THIN LAYER CHROMATOGRAPHY

3. THE PRINCIPLE SAMPLE APPLICATION CHROMATOGRAM DEVELOPMENT
CHROMATOGRAM EVALUATION

4. ADVANTAGES OFFERED BY HPTLC

5. SAMPLE APPLICATION / SAMPLE DOSAGE
NATURE OF THE ANALYTICAL TASK.
TYPE OF SEPERATION LAYER .
SAMPLE VOLUMES.

6. NANOMAT

7. Capillary Dispenser System

8. LINOMAT 1V

9. AUTOMATIC TLC SAMPLER

10. CHROMATOGRAM DEVELOPMENT
GENERAL

11. Ancillaries for Chromatogram Development in a tank

12. Flat Bottom Chamber

13. Twin Trough Chamber pre-equilibration with solvent vapor
               
pre-equilibration with solvent vapor
               
LOW SOLVENT CONSUMPTION

14.                
Start of development

15. LIGHT WEIGHT TWIN TROUGH CHAMBER

16. Horizontal Developing Chamber

17. Automatic Developing Chamber ADC

18. AMD System

19. DENSITOMETRIC CHROMATOGRAM EVALUATION
General, Comparsion of Video Technology with Classical Densitometry.

20. TLC Scanner 3 with “CATS” Soft Ware.

21. CATS STANDARD PROGRAM.
CATS PROGRAM OPTIONS

22. Photo & Video –Documentation / Video Densitometry.

23. Reprostar.

24. Video store.

25. Video Scan.

26. OPTIONAL (AUXILLARY) PROCEDURES:
UV –INSPECTION - UV Lamps.
POST CHROMATOGRAPHIC DERIVATIZATION.
OPTIMIZATION OF DEVELOPING CONDITION.
ALTERNATIVE TECHNIQUES FOR MNUAL SAMPLE APPLICATION.
IN HOUSE PREPARATION OF TLC PLATES.

27. UV –INSPECTION -UV Lamps.

28. POST CHROMATOGRAPHIC DERIVATIZATION
General.
Chromatogram Immersion Device.
TLC Sprayer.
Reagent Spray.
TLC Spray Cabinet.
TLC Plate Heater

29. Chromatogram Immersion Device.

30. TLC Sprayer.

31. Reagent Spray.

32. TLC Spray Cabinet.

33. TLC Plate Heater

34. OPTIMIZATION OF DEVELOPING CONDITION.
HPTLC VARIO SYSTEM.

35. ALTERNATIVE TECHNIQUES FOR MNUAL SAMPLE APPLICATION
Disposable Glass Capillaries
Graduated Disposable Micro Pipettes
Multipurpose Spotting Guide.

36. Disposable Glass Capillaries

37. Graduated Disposable Micro Pipettes

38. IN HOUSE PREPARATION OF TLC PLATES.
GENERAL.
Automatic TLC Plate Coater,
TLC PLATE Coater,
Hand operated,
Glass Plates ,
Drying rack,
TLC Plate Box .

39. Automatic TLC Plate Coater

40. TLC PLATE Coater, hand operated

41. TLC Plate Box.

42. Drying rack

43. ADSORBENTS FOR IN HOUSE PREPARATION OF TLC LAYERS.
General
CAMAG Adsorbents.
MERCK Adsorbents.

44. CAMAG Adsorbents.

45.
Aluminium Oxide CAMAG DS-0
1 kg
5 kg
Aluminium Oxide CAMAG DS-5
Aluminium Oxide CAMAG DSF-0
Aluminium Oxide CAMAG DSF-5
Cellulose, fibre type, CAMAG D-0
500 g
Cellulose, fibre type, CAMAG DF-0
Cellulose, microcrystalline, CAMAG DS-0
Cellulose, microcrystalline, CAMAG DFS-0
Silica Gel, CAMAG DS-0
Silica Gel, CAMAG DS-5
Silica Gel, CAMAG DSF-0
Silica Gel, CAMAG DSF-5

46. MERCK Adsorbents 033.1090 Aluminium Oxide MERCK G (type 60/E) 500 g
Aluminium Oxide MERCK GF (type 60/E)
2,5 kg
Aluminium Oxide MERCK H basic (type 60/E)
Aluminium Oxide MERCK HF basic (type 60/E)
Silica Gel MERCK G (type 60)
1 kg
5 kg
Silica Gel MERCK GF (type 60)
Silica Gel MERCK H (type 60)
Silica Gel MERCK HF (type 60)
Silica Gel MERCK HF (type 60)
Silica Gel MERCK 60 HR "high purity"
Kieselguhr MERCK 60 G

47. PRECOATED LAYERS

48. MERCK Precoated layers for HPTLC.
HPTLC plates cellulose F
100
10x10 25
20x10 50
HPTLC plates cellulose (without F)
HPTLC plates silica gel 60 (without F)
200
HPTLC plates silica gel 60 (without F), concentration zone
HPTLC plates silica gel 60 F 254
HPTLC plates silica gel 60 F 254 GLP
HPTLC plates silica gel 60 F 254, extra thin layer
HPTLC plates silica gel 60 F 254, concentration zone
HPTLC plates silica gel WR 60 F 254s
HPTLC plates LiChrospher® Si 60 F 254s
180
HPTLC aluminium sheets silica gel 60 (without F)
20x20

49. MERCK Precoated Layers for (Conventional) TLC.
TLC plates aluminium oxide   60 F 254
250
20x20 25
TLC plates cellulose F
100
TLC plates cellulose (without F)
TLC aluminium sheets cellulose (without F)
TLC plates silica gel (without F)
5x20
TLC plates silica gel (without F), concentration zone
10x20
TLC plates silica gel 60 F 254 50
TLC plates silica gel 60 F 254 GLP
TLC plates silica gel 60 F 254, concentration zone
TLC aluminium sheets silica gel 60 F 254
200
TLC aluminium sheets silica gel 60 (without F)
TLC aluminium sheets silica gel 60 (without F), concentration zone
TLC aluminium sheets silica gel 60, concentration zone
TLC plastic sheets silica gel 60 F 254
TLC plastic sheets silica gel 60 (without F)
TLC plates RP-18 F 254s

50. Test Dye Mixtures
Test Dye Mixture I 100 mL (suitable for silica gel) Dimethyl yellow - Oracet blue 2R - Oracet red G; solvent: toluene
Test Dye Mixture II 100 mL (suitable for silica gel and aluminium oxide) Sudan black - Artisil blue - Sudan yellow - fatty orange - fat red 7B - (Sudan black separates into two fractions); solvent: toluene
Test Dye Mixture III 10 mL (suitable for HPTLC silica gel) Ciba F II - Indophenol - Ariabel red - Sudan blue II - Sudan IV - dimethylaminoazobenzene; solvent: toluene
Test Dye Mixture IV 50 mL (MERCK 9352, hydrophylic) Chrysoin S - fast yellow - Amaranth - brilliant black BN; solvent: methanol - water 1:1

51. APPLICATIONS BIOCHEMICAL RESEARCH. CLINICAL. COSMETICS.
ENVIRONMENTAL ANALYSIS.
FOOD ANALYSIS.
PHARMACEUTICALS.
NATURAL PRODUCTS, PLANT INGREDIENTS.
TOXICOLOGY, FORENSIC ANALYSIS.
DOPING ANALYSIS.