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Published byMyrtle Bridges Modified over 9 years ago
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Introduction to Microscopy
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Objectives Learn to use a compound microscope correctly. Diagram the path of light through a compound microscope. Name major parts of a compound microscope. Identify three basic morphologies of bacteria.
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Size in Microbial World
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Magnification Refraction (bending) of light as it passes through the lens produces magnified image Two sets of lens contribute to total magnification Four objective lenses: 4x (scanning), 10x (low power), 40-45x (high power), 100x (oil immersion). Ocular lens: 10x
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Resolution Wavelength of light used Resolving Power = 2 x numerical aperture
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Qualities of a Good Image Brightness - related to NA. Focus - related to Focal Length. Resolution - related to NA & λ. Contrast - related to illumination (can be improved)
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Focusing and Iris Diaphragm
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