1. Why we propagate plants.
Propagation
Why we propagate plants.

2. Why propagate? Why propagate? 1. Ensure future generations.
2. Keeps desired combination of traits alive.
3. Do not have to depend on seed germination. Some plants have seeds that are hard to germinate. Also, do not have to worry about pollination.

3. Why propagate? Possible Problems 1. Will not have genetic variation.
2. Plants will not be able to change with the surroundings.

4. Types of Propagation Two main types of propagation. They are:
Asexual and sexual.
Asexual – cloning – does not require fertilization
1. Vegetative – reproduce by mitosis (each daughter cell is an exact replica of it’s mother cell. (cont.)

5. Types of Propagation
Chromosome numbers and composition do not change during cell division). Ex: stolons and rhizomes.
2. Cuttings – taking parts of plants and planting them
Stem cuttings
Leaf cuttings

6. Stem Cutting with terminal growing area.

7. Leaf cutting without petiole.
Leaf cutting with petiole.
Leaf Cuttings
Leaf cutting that has rooted. Used leaf with petiole.

8. Leaf Cutting
Leaf cutting with the petiole attached. Placed in water and will result in roots being formed.
Leaf cutting with petiole attached but placed in soil. This will be the result.

9. Asexual Cuttings Stem cuttings Hardwood Semi-hardwood Soft wood
Herbaceous
Leaf cuttings
Leaf-bud cuttings
Root Cuttings

10. Stem cutting showing an adventitious bud starting. Begonia plant.
Stem cutting with an auxiliary bud attached.
Stem Cuttings

11. Leaf Bud Propagation
For this type of propagation, you use a leaf and the axil of the leaf along with a portion of the stem. Place all of this into a medium and let grow. Type of plants you can use this on are: clematis, grape ivy, dracaena, camellia, jade plant and rhododendron.

12. Asexual Cuttings 3. Grafting – using woody stems Why do we graft?
Things to remember when grafting.

13. Asexual Cuttings
3. Grafting – when grafting, you use some part of the woody stem. There are many types of grafts. The following pictures show a procedure called whip grafting.

14. Whip Grafting Step 2 - Tools
Step 3 - Cutting stem to make area for grafting.
Step 1 -Selection
Whip Grafting

15. Step 5 – Adding the cut down through the middle.
Step 4 - Showing how to make the cut.
Step 6 – Making the cut on the grafting material.

16. Step 7 – Making incision into the graft.
Step 8 – Putting the two grafts together.

17. Step 9 – Wrapping the graft.
Step 10 – Finished product.

18. Propagation Methods 4. Budding – inserting bud into existing wood
5. Layering – taking a stem and promoting root growth.

19. Layering – Simple or mound
Layering – taking a branch and placing it on the soil.

20. Air Layering Removing epidermis for layering.
Packing moss around area to provide moisture.
Wrap in saran wrap to keep moisture in.
Air Layering
Removing saran wrap to see new roots and bud.
New bud with roots.

21. Separation Method
7. Separation – you take mature plants that were stolons or offspring from a parent plant and separate.

22. Runners
6. Runners – stems that run along the top of the ground. They root at the node.

23. Stolons – Parent plant puts out runners and each node a new plant forms along with roots.
Runners or Stolons

24. Separation of runner from parent.
Separation Method

25. Division
8. Division – taking tubers, roots, bulbs, or corms and dividing them.

26. Stem Tubers – potato – eyes become plants.
Division

27. Division
Corms – roots develop around the edge in a circle. The enlarged area is the stem and the leaf sprouts in the concave area on top. Each one of these leaves will become a corm next year. Crocus and glads are examples.

28. Suckering
Suckers – growth from the root or crown area. Would use division here because of the roots.

29. Suckering – where plants are produced from roots
Suckering – where plants are produced from roots. Raspberries, willows and dog woods are examples.
Suckering

30. Tissue Cultures 9. Micro propagation – Tissue cultures
Tissue cultures are taking a small part of a leaf or stem (usually cut into small pieces), placing them in a petri dish that contains agar (medium that has nutrients added to it).

31. Micro propagation
Micro propagation – using a part of the plant such as a piece of the leaf (African violet) and placing it in a Petri dish that has agar in it. Roots form from this piece of leaf.

32. Factors Influencing Rooting
Factors influencing the rooting of cuttings.
1. Source of cutting material.
Young, non flowering plants only a few years away from a germinated seed is best.
2. Time of year the cutting material is taken.
Woody perennial (means grows for a number of years)- anytime

33. Factors Influencing Rooting
Hardwood cuttings – late winter
Softwood cuttings – spring after growth reaches 4-6”.
Semi-hardwood cuttings – midsummer
Herbaceous – anytime of the year (do not develop woody tissues).

34. Factors Influencing Rooting
3. Treatment of cuttings with Auxins (natural plant hormones)
IAA (indoleacetic acid), IBA (indolebutric acid) or NAA (naphthalenoeacetic acid
All stimulate root growth

35. Factors Influencing Rooting
4. Misting – needed in herbaceous cuttings. Need to keep hydrated so will not dry out.
Mist the plant then cover with plastic cover. Keeps moisture in.
Can also have an automated mist system.
5. Bottom heat – helps force rooting by stimulating the root to grow.

36. Sexual Propagation Seed formation is an example of sexual propagation
Get genetic diversity from this form
Where you have a male and a female and have cross pollination for fertilization between the two to form the seed.
Meiosis – another form of sexual propagation

37. Sexual Propagation
In meiosis the number of chromosomes in the cells is reduced by half to form the male sperm cell and the female egg. The egg and sperm combine during fertilization in the ovule to form the zygote (fruit) that develops into the embryo (seed).
California desert plant called Machaeranthera gracilis example

38. Seed Germination Monocot Seed
The seed imbibes (absorbs) water from the soil
Emergence of radicle (primary root) and the plumule (primary shoot)
These form the primary parts of the plant.

39. Monocot Seed

40. Dicot Seed Dicot Seed Seed imbibes water from soil and swells.
Radicle emerges and grows downward.
Hook of the bean (hypocotyl) emerges from soil, carrying the two cotyledons (leaves) with it.

41. Dicot Seed

42. Environmental Factors Influencing Seed Germination
Adequate moisture in the soil.
Proper temperature – cold versus warm
Good aeration of soil.
Light (in some cases)
Freedom from pathogenic organisms. Damping off.
Freedom from toxic amounts of salts.