1. Filling a Rack Matrix with Solvent Needs version 1.1.3.8A

2. 2 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 Introduction This method is useful, if you want to: Dispense solvent in a rack. Dissolve solid samples that are supplied in a rack. Dilute samples that are supplied in a rack. This document describes How a complete rack or several vials in a rack can be filled with a user defined amount of solvent (Solvent from dilutor or small amounts from tray). Two procedures will be used: The first fills the syringe with the total solvent amount. The second quickly dispenses equal solvent parts into the selected destinations. Tip: Prep-Gilson always requires source and destination positions. In this case only the second procedure needs the selection of destination positions.

3. 3 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 2. ”Manual destination selection“ is checked. 3. ”Clean... “ is unchecked. Step 1: Check Settings

4. 4 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 Open procedure editor dialog Two main procedures will be defined: 1. Fill syringe with the total solvent amount 1200µl for 12 tubes. Name: ”Load Syringe DMSO 1200.gsp“ 2. Dispense partial solvent amount 100µl into one destination. Name: ”Dispense Volume 100.gsp “ (with subprocedure ”Sub Disp Volume 100.gsp “) Step 1: Check Settings

5. 5 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 2. Select the source type ”Solvent in Tray“. 3. Select your own values for: - Solvent name - Volume (Speed...) Tip: Never forget ”Update“ and ”Save“ after any modifications. 1. Check the file name ”Dispense Volume 100.gsp“ Step 2: Edit Procedure ”Load Syringe DMSO 1200.gsp“

6. 6 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 Press the ”Edit“ button to edit or create a procedure of type ”Dispense“. Step 3a: Edit Dispense Subprocedure ”Sub Disp Volume 100.gsp“

7. 7 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 Select your own values for: - Volume - Z offset height (Speed...). Check the file name ”Sub Disp Volume 100.gsp“. Step 3a: Edit Dispense Subprocedure ”Sub Disp Volume 100.gsp“

8. 8 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 Select the source type ”Gas Gap“. Select your dispense procedure. Check the file name ”Dispense Volume 100.gsp“. Volume = 0 Step 3b: Edit Dispense Main Procedure ”Dispense Volume 100.gsp“

9. 9 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 Start Automation. 1.Select procedure ”Load Syringe DMSO 1200.gsp“. 2. Select one dummy source and destination position (ones not used in the procedure!). Tip: Solvent not used in procedure! 3. Create Order and do not start with ”OK“. Step 4: Start Automation to Fill Sample Loop

10. 10 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 1.Select procedure “Dispense Volume 100.gsp“. 2. Select 12 source and destination positions (destinations will be used). Tip: Solvent not used in procedure! 3. Create orders and close dialog with “OK“. Step 5: Create Orders to Fill Rack Matrix

11. 11 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48 Step 6: Running Preparation

12. 12 Filling a Rack Matrix with Solvent in Two Steps - Fast 15.08.2015 04:48