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Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds by Elizabeth Bilsland, Andrew Sparkes, Kevin Williams, Harry J.

Published byEthan Strickland Modified over 9 years ago

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Presentation on theme: "Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds by Elizabeth Bilsland, Andrew Sparkes, Kevin Williams, Harry J."— Presentation transcript:

1 Yeast-based automated high-throughput screens to identify anti-parasitic lead compounds by Elizabeth Bilsland, Andrew Sparkes, Kevin Williams, Harry J. Moss, Michaela de Clare, Pınar Pir, Jem Rowland, Wayne Aubrey, Ron Pateman, Mike Young, Mark Carrington, Ross D. King, and Stephen G. Oliver Open Biology Volume 3(2):120158 February 27, 2013 ©2013 by The Royal Society

2 Fluorescence labelling of yeast strains. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

3 Relative fluorescence measure detected using a BMG Optima plate reader at 580 nm (excitation)/612 nm (emission) (Cherry), 405 nm (excitation)/510 nm (emission) (Sapphire), 500 nm (excitation)/540 nm (emission) (Venus) and 440 nm (excitation)/490 nm (emissio... Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

4 Example of a high-throughput screening result. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

5 Network of connections between kinetoplastid targets and our hits. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

6 Antiplasmodial hits. Elizabeth Bilsland et al. Open Biol. 2013;3:120158 ©2013 by The Royal Society

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