1. Experiment Questions
Enzyme Temperature

2. Enzyme Temperature
State one factor that you kept constant during the investigation. pH or substrate conc. or enzyme conc.

3. How did you vary the temperature? Water baths (at different temps.)
Enzyme Temperature
How did you vary the temperature? Water baths (at different temps.)

4. Enzyme Temperature
How did you know that the enzyme had completed its activity? No more product/ Colour change/ No more bubbles/ No more foam

5. Name the enzyme that you used. Name of enzyme, e.g. catalase
Enzyme Temperature
Name the enzyme that you used. Name of enzyme, e.g. catalase

6. Enzyme Temperature
Name the substrate of the enzyme that you used. Name of matching substrate, e.g. hydrogen peroxide

7. Enzyme Temperature
A number of factors were kept constant during this experiment. Explain how you kept one of them constant? pH—used a buffer substrate conc.—same volume enzyme conc.—same amount

8. Enzyme Temperature
How did you measure the rate of activity of the enzyme? Time / change e.g. colour, foam, etc or data logger / sensor named

9. Enzyme Temperature
What was the result of your investigation? Activity varies with temperature or reference to activity at a particular temperature

10. Enzyme Temperature
(i) Name an enzyme that you used. (ii) Name the substance that this enzyme acted on. (i) Catalase or amylase (ii) H2O2 or starch (must match enzyme)

11. Enzyme Temperature
How did you vary the temperature? Water baths/ at different temperatures/ thermostat

12. Enzyme Temperature
How did you measure the rate of the enzyme’s action? Volumes of foam/ per minute/ disappearance of blue colour/ per minute

13. Enzyme Temperature
(i) What enzyme did you use? (ii) What substrate did you use? Name of (i) enzyme, e.g. catalase (ii) substrate, e.g. H2O2 —must match

14. Enzyme Temperature
How did you know that the enzyme had completed its activity? No more product /colour change / no more bubbles / no more foam

15. Enzyme Temperature
How did you vary the temperature in your experiment? Water bath / different temperature treatments / Bunsen / thermostat

16. Enzyme Temperature
(i) Name the enzyme that you used. (ii) Name the substrate with which the enzyme reacts. (i) Name of enzyme (ii) Name of substrate [must match enzyme]

17. How did you vary the temperature? Water bath or ice or thermostat
Enzyme Temperature
How did you vary the temperature? Water bath or ice or thermostat

18. How did you keep a constant pH during the investigation? Buffer
Enzyme Temperature
How did you keep a constant pH during the investigation? Buffer

19. Enzyme Temperature
How did you measure the rate of activity of the enzyme? Time / change (colour, height of foam, etc.) OR sensor / data logger

20. Enzyme Temperature
What was the result of your investigation? Activity increases or enzyme has an optimum or graph showing increase

21. Enzyme Temperature
Name the enzyme that you used. Name the substrate of this enzyme. e.g. Enzyme – catalase; Substrate - Hydrogen peroxide

22. Enzyme Temperature
Why was it necessary to keep the pH constant in the course of the investigation? To eliminate it as a possible influence on rate or only one variable

23. How did you keep the pH constant? Buffer
Enzyme Temperature
How did you keep the pH constant? Buffer

24. Enzyme Temperature
How did you vary the temperature in the course of the investigation? Water baths or water bath at different temperatures

25. Enzyme Temperature
How did you know that the enzyme was working? Description of visible result matching enzyme (or substrate)

26. Enzyme Temperature
What enzyme did you use? What substrate did you use? e.g. Enzyme – Catalase Substrate – Hydrogen peroxide (Must match enzyme)

27. How did you vary the temperature during the investigation? Water bath
Enzyme Temperature
How did you vary the temperature during the investigation? Water bath

28. Enzyme Temperature
How did you measure the rate of enzyme activity? e.g. Volume of froth / time (Depends on enzyme used)

29. Enzyme Temperature
During this investigation pH was kept constant. How did you keep the pH constant? Buffer

30. Enzyme Temperature
What was the result of your investigation? Increasing activity with increasing temp / works best at certain temp / activity decreases above or below certain temp. (Can be shown by graph with one axis labelled)