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An introduction to a novel filtration system ATF-manufacturing Platform Upstream Unit Operations ATF-cellcultivation Concentrated Perfusion Concentrated.

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Presentation on theme: "An introduction to a novel filtration system ATF-manufacturing Platform Upstream Unit Operations ATF-cellcultivation Concentrated Perfusion Concentrated."— Presentation transcript:

1 An introduction to a novel filtration system ATF-manufacturing Platform Upstream Unit Operations ATF-cellcultivation Concentrated Perfusion Concentrated Fed-Batch Hybrid Processes

2 UF filter concentrates protein inside reactor Filtrate pump runs at 0.5-3 vv / day or as required by cell growth ATF rate constant at ~0.05-0.1 vv / min (or faster) Feeding controlled by level probe Concentrated Fed-Batch (CFB) Has the benefits of Concentrated Perfusion (CP) and Fed-Batch together –high cell concentration (70-200m/ml) achievable in 2 weeks Straight-forward feeding control & removal of unwanted metabolites Concentrated harvest in one identified batch: CHO at 17g/L (and higher) CFB allows retention of some protecting proteins and growth factors so viability may be higher than CP, removing the need for a bleed and simplifying the process control

3 CFB Results – PER.C6 Cells

4 CFB Results – CHO Cells


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