1. Active Transport 1. A cell contains a solution that is 88% water, and it is sitting in a solution that is 20% water. 2. A cell contains a solution that is 43% sugar, and it is sitting in a solution that is 99% sugar. When you are done, begin to read through the Egg Lab packet.  Identify the type of solution  Describe what will move, and where  Explain how the cell’s volume will change  Identify the type of solution  Describe what will move, and where  Explain how the cell’s volume will change

2. Agenda  Catalyst  HW review  Egg Lab!  Active Transport  EQ

3. Homework Review  http://www.coolschool.ca/lor/BI12/unit 4/U04L06/rbc.html http://www.coolschool.ca/lor/BI12/unit 4/U04L06/rbc.html

4. HYPOTONICISOTONICHYPERTONIC “LYSIS”

6. Yesterday… We learned about OSMOSIS!

7. Egg Lab – Day 1  An egg can be thought of as a big cell. After the shell is removed (with vinegar), it is a partially permeable membrane.  Putting this egg in a hyper/hypotonic solution will cause osmosis to occur.

8. Egg Lab – Day 1 SET UP: 1. Observe each egg. Record your observations in Data Table 2. 2. Calculate the mass of each egg. Record this mass in Data Table 1. 3. Label one beaker A and one beaker B. Put one egg in each. 4. Add enough acetic acid to the beaker so that you are completely covering the egg. 5. In Data Table 2, record detailed observations of the eggs.

9. How do you use a triple-beam balance?  Calibrate using knob on the left! Make sure balance is pointing at 0 before you start.  Put object on metal balance.  Start with the highest beam (100 g) increments. Move it over one by one notch until the balance goes below 0. Do this for each beam until you have you balance at 0.

10. What if we want to take the mass of an egg?  It might roll off and die!  Correct method:  Take mass of the beaker. (WRITE IT DOWN – Data Table 1)  Take the mass of the beaker and the egg inside it.  Subtract the first mass from the 2 nd to get the mass of the egg. Mass of Beaker+Egg – Mass of Beaker = Mass of Egg

11. Egg Lab – Day 1 QUESTION: Will an unshelled egg increase or decrease in mass after being placed overnight in corn syrup or water?

12. Egg Lab – Day 1 HYPOTHESIS: Formulate two hypotheses, one for putting an egg in water, the other for putting an egg in corn syrup. (Formulate means “create.”) Example: 1. If the egg is placed in water, then it will increase/decrease in mass because ____________. 2. If the egg is placed in corn syrup, then it will increase/decrease in mass because ____________. CHOOSE ONE

13. Egg Lab – Day 1 VARIABLES:  Identify your INDEPENDENT and DEPENDENT variables.  Identify at least 2 CONSTANTS. REMEMBER: Variables are categories, not specific things!

14. Objectives  By the end of the period, SWBAT…  Compare active and passive transport  Identify whether cellular transport is diffusion, active transport, or osmosis

15. Passive Transport  Key Point #1: Passive transport takes no energy and always moves towards equilibrium.  The goal is equal concentration  High  Low concentration  Happens naturally (no energy)  BOTH diffusion AND osmosis are passive transport

16. In DIFFUSION  The solutes are permeable to the membrane and move H  L.

17. In OSMOSIS  Only water is permeable to the membrane. 75% water 20% water

18. Active Transport  Key Point #2: Active transport takes energy to move all solutes in or out of the cell.  The goal is to move against equilibrium  Low  High concentration  Happens on purpose (takes energy)

19. In ACTIVE TRANSPORT  The solutes are permeable to the membrane and move L  H

20. Active Transport  Why would the cell do this?  To store food inside the cell  To eliminate wastes  To get rid of toxins

21. Comparing the Three DIFFUSION ACTIVE TRANSPORT PASSIVE TRANSPORT OSMOSIS SOLUTES WATER EQUILBRIUM NO ENERGY SOLUTESEQUILBRIUM ENERGY

22. Helpful Hints  How can you tell if something is active or passive?  How do you know if it diffusion or osmosis?  Ask yourself:  Is the solute moving, or is water moving?  What is permeable to the membrane?  Are we moving H  L or L  H?

23. Guided Practice  State whether this is Active Transport or Passive Transport

24. Active or Passive?

26.  Water moving to equalize concentrations

27. Active or Passive?  Cell uses energy to get as much sugar as possible

28. Active or Passive?  Happens naturally with no use of energy

29. Active or Passive?  ALL solutes being moved out of the cell

30. Active or Passive?  Takes energy

31. Active or Passive?  Moving toward equilibrium

32. Changing Gears  Now I will give you two cells. I will tell you what is moving and where. You tell me if it is DIFFUSION, OSMOSIS, OR ACTIVE TRANSPORT.

33. If solutes are moving from L  R

35. If water is moving from R  L

36. Water is moving from inside  outside  A cell has a 40% sugar concentration inside is in a solution of 50% sugar concentration  HINT: IT MIGHT HELP YOU TO DRAW THE SITUATION

37. Solutes are moving from inside  outside  A cell has a 10% sugar concentration inside is in a solution of 50% sugar concentration

38. Solutes are moving from outside  inside  A cell has a 10% sugar concentration inside is in a solution of 80% sugar concentration

39. Solutes are moving from outside  inside  A cell has a 40% sugar concentration inside is in a solution of 20% sugar concentration

40. Key Point Wrap-Up  Key Point #1: Passive transport takes no energy and always moves towards equilibrium.  Both diffusion and osmosis  Key Point #2: Active transport takes energy to move all solutes in or out of the cell.

41. Exit Question 1. Match each of the following statements to either ACTIVE or PASSIVE transport. (You do not have to write complete sentences!) (1) Requires energy (2) Movement of solute or solvent in/out of a cell (3) Moves toward equilibrium 2. A cell containing 20% sugar solution is placed in 80% sugar solution. What will happen if ACTIVE transport occurs?