1. Microbiology Laboratory Safety and Rules Biology II Mrs. Hieneman

2. #1 Do not bring food, drink, or tobacco into the lab.Do not bring food, drink, or tobacco into the lab. Do not invite friends into the lab.Do not invite friends into the lab. Keep pencils, fingers, etc. out of your mouth, ears, eyes, nose, etc.Keep pencils, fingers, etc. out of your mouth, ears, eyes, nose, etc.

3. #2 Wear protective clothing at all times.Wear protective clothing at all times. Shoes are always requiredShoes are always required Open-toed sandals are not allowed.Open-toed sandals are not allowed.

4. #3 Do not take any cultures out of the lab for any reason.Do not take any cultures out of the lab for any reason.

5. #4 Wipe the bench tops down with disinfectant both before you begin your work and after you have completed your work.Wipe the bench tops down with disinfectant both before you begin your work and after you have completed your work.

6. #5 Keep nonessential books and clothing far away from your work area.Keep nonessential books and clothing far away from your work area.

7. #6 Wash your hands with soap and water for 15 seconds before and after you work in the lab, or if you leave the lab for any reason.Wash your hands with soap and water for 15 seconds before and after you work in the lab, or if you leave the lab for any reason.

8. #7 Do not mouth pipet ever!!!!!!!!!!Do not mouth pipet ever!!!!!!!!!!

9. #8 Be careful near bunsen burners.Be careful near bunsen burners. Tie long hair back away from the face.Tie long hair back away from the face. Turn off burners when not in use.Turn off burners when not in use.

10. #9 Beware of the chemicals and reagents used in biochemical tests.Beware of the chemicals and reagents used in biochemical tests.

11. #10 Report any accidents (spills, broken glass, cuts, or injuries) to your instructor immediately.Report any accidents (spills, broken glass, cuts, or injuries) to your instructor immediately.

12. #11 Dispose of contaminated or used materials quickly and in the manner set forth by your instructor.Dispose of contaminated or used materials quickly and in the manner set forth by your instructor.

13. #12 Label all of your tubes and plates exactly as directed by your instructor.Label all of your tubes and plates exactly as directed by your instructor. Place all cultures for incubation exactly where instructed or you risk their loss.Place all cultures for incubation exactly where instructed or you risk their loss. Any cultures not handled accordingly will be discarded!!!Any cultures not handled accordingly will be discarded!!!

14. #13 Keep microscopes clean and the objectives free of oil.Keep microscopes clean and the objectives free of oil.