1. Measuring volumes which are what we scientists call “very, very small”
Micropipettes
Measuring volumes which are what we scientists call
“very, very small”

2. MICROPIPETTE RULES
Don’t set the pipettes past their intended range (high or low), even though you can.
ALWAYS return the dial to the highest setting before putting the pipette away.
ALWAYS return the pipette to its correct storage bin

3. High Range: µL
Use large blue or white tips
Digits from top to bottom represent:
1000’s
100’s
10’s
The 1’s are indicated by the tiny white tick marks in the dial with the 10’s. Marks are in increments of 2 µL
This pipette is set to 1000 µL

4. This pipette is set to
998 µL

5. This pipette is set to
990 µL

6. Mid-range:
µL µL, µL
Range is printed on the button or on the side
Use medium sized yellow or white tips

7. µL
This pipette is set to its maximum:
100.0 µL
Digits represent:
100’s
10’s
1’s
Tick marks represent 0.1’s in increments of 0.2 µL

10. Low Range: µL µL
Digits represent:
10’s
1’s
0.1’s
Tick marks are 0.01’s in increments of 0.02 µL
Use small white tips

11. Rainin 0.50-10.00 µL pipettes use a unique tip
Clear tip has an extra wide mouth that surrounds and goes over the barrel and eject bar.
These tips can ONLY be used with the Rainin

12. Feel the stops before you begin taking up or dispensing any liquid.
1st stop: Exact volume to which the pipette is set. Press to this stop before immersing pipette tip in the desired solution.
2nd stop: Use when dispensing solution. Pushes an extra volume of air to dislodge any solution adhering to the tip.

13. How to pipette
Great summary

14. Extract and dispense SLOWLY.
When dispensing, touch the tip
to the side or bottom of the tube,
or if there is liquid already in the
tube, touch the tip to the liquid.
When taking up or dispensing
liquid, don’t put the tip any deeper
into the liquid than necessary.

15. NEVER hold pipette so that the tip is higher than the handle or liquid leaks into the barrel (BAD!)
Always hold pipettes vertically
when in use.
Check to be sure the tip is on securely.
Only open the tip box when getting a tip – DON’T LEAVE IT OPEN!