1. COLUMBIA UNIVERSITY Department of Chemistry Laboratory Safety Luis Avila 455 Chandler Labs avila@chem.columbia.edu C2507-2004

2. 1.The Hazard Communication Standard 2.Proper Work Practices 3.Engineering Controls-Chemical fume hoods 4.Personal Protective Equipment (PPE) 5.Hazardous Chemical Waste Procedures 6.Medical Emergencies 7.Emergencies Involving Hazardous Materials 8.Fire Safety Standards for Occupational Exposure to Hazardous Chemicals in Laboratories

3. NFPA Hazard Diamond Lab Safety Supply Inc. http://www.labsafety.com/ HMIG (Hazardous Material Identification Guide) MSDS (Material Safety Data Sheets) http://www.msdssearch.com/DBLinksN.htm The Hazard Communication Standard NFPA (National Fire Protection Association) Hazard Identification System http://www.nfpa.org/Home/index.asp

4. NFPA 704 Hazard Identification System NFPA Hazard Diamond Flammability Reactivity Health Special Precautions

5. Flammability Aluminum chloride Rapidly vaporize at normal conditions, or that are readily dispersed in air and that will burn readily 4 Ethyl ether Can be ignited under almost all ambient temperature conditions. 3 Diesel fuel oil Relatively high ambient temperature before ignition can occur 2 Melting point apparatus oil Pre-heated before ignition can occur1 WaterWill not burn0 ExamplePropertiesNumber

6. Health NumberPropertiesExample 0 Harmful on exposure under fireCorn oil 1 On exposure would cause irritation with minor residual injury Chloroform 2 Intense or chronic exposure could cause temporary incapacitation or possible residual injury Ammonia gas 3 On short exposure could cause serious temporary or serious injury Chlorine gas 4 On very short exposure causes death or major residual injury Hydrogen Cyanide

7. Reactivity NumberPropertiesExample 0 Normally stable even under fireLiquid nitrogen 1 Becomes unstable at elevated temperature and pressures Red or white phosphorus 2 Violent chemical change at elevated temperatures and pressures or which reacts violently with water, or which may form explosive mixtures with water  Calcium metal 3 Detonation or explosive decomposition. Requires a strong initiating source or which must be heated under confinement before initiation or which reacts explosively with water Sodium azide 4 Capable of detonation or explosive decomposition or reaction at normal temperatures and pressures  Trinitrotoluene  (TNT)

8. Special Precautions SymbolPropertiesExample Unusual reactivity with waterAlkali metals OX Unstable at elevated temperature and pressuresAmmonium nitrate ACID Acidic material  Hydrochloric acid ALK Alkaline material Sodium hydroxide COR Corrosive Material  Chromic acid Radioactive materialPlutonium Metal

9. Hazard Material Identification System (HMIG Label) Lab Safety Supply Inc.

10. PPE symbols used in HMIG Apron Dust respirator Vapor respirator Face shield Splash Goggles Dust and Vapor respirator Airline hood or mask A B C D E F G H I J K X Ask supervisor or EHRS specialist

11. Section 1: Chemical Identity The material's chemical and common names, and a list of all hazardous ingredients. Section 2: Ingredients and Occupational Exposure Limits Legal exposure limits (OSHA and other recommended limits). This frequently includes toxicity information Section 3: Physical Data Physical and chemical characteristics such as vapor pressure, flash point, density, boiling point Section 4: Physical Hazards Fire and explosion data. Reactivity data. Signs and symptoms of exposure. Section 5: Reactivity Data Section 6: Health hazard data Carcinogenicity Summary of Risks Primary Entry Routes. The route(s) by which the material could most likely enter the body Section 7: Spill, leak, and disposal procedures: Hygienic practices, protective measures required during handling of contaminated equipment, and procedures for clean-up of spills and leaks. Section 8: Special Protection data Any control measures known to the party preparing the MSDS, such as engineering controls, work practices, or personal protective equipment. Section 9 Special Precautions and comments: Storage requirements, engineering controls, administrative controls Material Safety Data Sheet (MSDS) http://www.hr.columbia.edu/ehrs/html/msds.html

12. Toxicity Values found in MSDS MaterialLD50 (mg/kg)subject, route Pointer Sucrose29700 rat, oral MSDS Sodium bicarbonate4220 rat, oral MSDS Sodium chloride 3000 rat, oral MSDS Ethanol2080 rat, oral MSDS Caffeine192 rat, oral MSDS Sodium cyanide6.4 rat, oral MSDS Sarin (nerve agent) 2.4 human, percutaneous MSDS VX (nerve agent0.14 human, percutaneous MSDS

13. Proper Work Practices in the Laboratory  Proper Handling of Chemicals and Equipment  Conduct, Behavior  Attire

14.  Do not store chemicals or equipment on the floor  Maintain adequate aisle space  Know location of protective equipment -Fire extinguisher -Safety Shower -Eye Wash -Spill Response Kit -First Aid Kit Proper Work Practices in the Laboratory

15. Equipment Handling Lasers (Power & Wavelenght) Class I < 0.9mW (CAUTION) Class II 0.9mW (CAUTION) Class IIIa 4mW (CAUTION) Class IIIb 50mJ (DANGER) Class IV 50W(DANGER) Avoid reflective surfaces Wear Safety Goggles with low band pass filter (Ex. 1.064µ) Avoid skin contact

16. Equipment Handling  Valve regulators  Gas Cylinders  Venting Hoods  Power Supplies  Vacuum and High Pressure Lines

17.  Maintain clear access to all protection equipment  Keep lab door closed Accessibility

18.  Keep hoods free of unneeded chemicals and apparatus  Work in hoods with sash set to proper height  Check that fume hood is in proper working order before using  Have all required and necessary hazard placards Chemical Fume Hoods

19.  Store cylinders not being used in designated areas, not in the Lab  Do not secure cylinders to electric conduits or plumbing  Keep all cylinders secured and standing upright  Transport cylinders with proper hand trucks and with the cap on Gas Cylinders

20. Hazardous Chemical Waste Procedures Environmental Health and Radiation Safety (EHRS) Division at Columbia University General EHRS Office Numbers: X4-8749, X44658 http://www.hr.columbia.edu/ehrs/html/hazardous_regulated_waste.html

21. Fire Safety. Combustion Oxidizer HeatReducer http://www.hr.columbia.edu/ehrs/html/fire_safety.html

22. Reporting a Fire  Incidents not related to Columbia University Dial 911  Campus Rolm phone system Dial 99  Manual Pull Stations Located at Exits

23. Medical Emergencies  Incidents not related to Columbia University Dial 911  Campus Rolm phone system Dial 99  Columbia Area Volunteer Ambulance http://www.columbia.edu/cu/cava/home.html

24. When to Sound a Fire Alarm  ANY Fire or Smoke Condition  Dangerous Situation Crime in Progress, Suspicious activities  For Emergency Building Evacuation

25. Laboratory Evacuation Procedures  Turn Off All Apparatus Within Reach That Can Be Safely Turned Off  Close All Chemical Containers That You Are Working With  Close Fume Hood Sash

26. Laboratory Evacuation Procedures  Know Location Of Fire Exit, Count Number of Doors  Look For Exit Sign  Use Nearest Stair  Do Not Use Elevator Exit Building And Move Away From Door To Allow Security And Fire Department Access

27.  Feel Door, If Hot, Do not Open  If Smoke or Heat is Present, Remain in Room, Close Door, and Seal Cracks  If Possible Phone Security and Give Your Location  Stand at Window to Signal Security Or Fire Dept  Report any People That Might Still Be Inside  If You Catch Fire, Stop-drop-and-Roll  Never Jump Or Climb Out A Window In the Event of a Real Fire

28. Fire Extinguisher Types Class Of Fire Class A, Combustibles, Paper Class B, Grease And Oil Class C,D Electrical Extinguisher Types Pressurized Water Dry Chemical, B-C Or A-B-C-D Check Label. CO 2 Class B -C