1. Data Collection

2. Task 1. Spot the First Mosquito of the Year Goal: Record the first spotting of mosquitoes of 2014. Materials Needed: First spotting Data Sheet to record information. Guidelines and Research: Individuals will mark down on the data sheet the date, time, location, and description of the first mosquito they see in their every day lives in 2014. Individual’s are only recording mosquitoes that they notice within the city limits of Baltimore, Maryland. 2

3. Task 1. Spotting the First Mosquito of the Year 3

4. Task 2. Spotting mosquito habitats Goal: Individual’s will be able to highlight key problem areas for mosquito growth. Materials Needed: --Survey Data Worksheets Guidelines and Research: Individuals will be able to identify a mosquito lifecycle, which includes the habitats that mosquitoes live in and need for reproduction. They will then complete an initial physical and biological assessment of their field site using a data collection form. During this initial survey, individuals will also note potential mosquito breeding habitats. 4

5. First look at the mosquito stoppers descriptions. 1. Considering the property of your residence, develop a list of areas you think are potential breeding habitats for mosquitoes. * Breeding habitats can include natural and man- made water bodies (e.g. trash, tires, play structures, potted plants, etc). 3. Take your site survey form and note both biological and non-biological features of the sites you have selected. Regarding site ID please provide the street address (note: this information will only be used to map mosquito habitats). Also mark down the number and type of potential mosquito breeding habitats. 5 Task 2. Spotting mosquito habitats

6. 6 Task 1. Mosquito Habitats Mosquito Habitats

7. 7 Site within yard Air Temp (F) Water Presence ? (Y/N) Habitat diameter Habitat Type Physical NotesBiological Notes Task 2. Spotting mosquito habitats (take multiple sheets) Site ID: _____________________________________________

8. 8 Task 3: Sampling for Larvae Also called wrigglers! Imagine small, the size of a pencil tip, creatures that wriggle from side to side. Visit: http://youtu.be/QRp v7aNtQiY http://youtu.be/QRp v7aNtQiY

9. Task 3. Sampling for mosquito larvae Counting Mosquito Wrigglers Protocol Goal: To sample mosquito wrigglers in breeding habitats around Baltimore, Maryland. Read over materials and data collection sheets before starting your own sampling. 1.Sample during the first week of May 2014. 2.On top of Wriggler Data Sheet, write your street address in the ID section (all information given in this project is strictly confidential). Do this for all sheets every sampling time. Write the date on every sheet for each sampling time. 9

10. Task 3. Sampling for mosquito larvae 3. To sample: -Begin by walking around the property of your residence and note any potential habitats. Be sure to review the Mosquito Habitat Identification worksheet, which outlines examples of mosquito habitats and how to measure them. -Designate 5 to 10 potential habitats around the property you think would have wrigglers. Make sure to remember where/what these habitats are. You are only sampling wrigglers from wet habitats with standing water. -Each mosquito habitat will be successively numbered under Plot ID starting from 1. -For each mosquito habitat with standing water, you will take a 1mL sample of water for a visual inspection for wrigglers. To take the sample, squeeze the bulb of the pipette and insert the tip into the standing water. With the tip under water, release the bulb and the pipette should fill with water. Empty the pipette onto a petri dish and visually inspect the sample. Count the number of wrigglers in sample and record on Wriggler Data Sheet. Empty petri dish and rinse with water before moving onto next sampling area. -Continue along your route, repeating these directions until you have returned to the starting location. 4. For each sampling period, you should use the same sampling technique and follow the same route. There may be changes in the number, state, or location of mosquito habitats between sampling periods and this is normal. 10

11. Activity 3. Urban Site and Mosquito Field Study 11 Mosquito Lifecycle

12. 12 Task 3. Sampling for mosquito larvae Wriggler Data Sheet ID= Habitat Codes: Date= 1= Puddles 4=Buildings/Foundations7=Tires 2= Swampy Areas 5=Water Storage Container8=Planters 3=Drain/Ditch 6=Trash 9=Other Plot IDHabitat IDHabitat DescriptionVisual EstimatePlot IDAre there wrigglers present?Number from sample /////////////// ////////////////////////////////////(Yes/No)///////////////YesNo1mL sample

13. Nuisance Survey Date__________ (May ______, June _____, July _____, August_____) Instructions: Please circle or check the most appropriate response for what has occurred in the past TWO WEEKS. Note: While encounters with mosquitoes differ from day to day, try to think about nuisance in general.. 1. How often did you spend time outdoors (i.e., beyond a 30 minute period)? ____Daily____A few times a week____Once a week ____Rarely____Never 2. When you do spend time outdoors, on average, how many hours do you spend? _____ 3. Did mosquito-related nuisances affect the amount of time you spent outdoors? ____Yes____No____Unsure Explain your answer and please provide other factors that may have kept you indoors, when you would have preferred to be outdoors. ______________________________________________________________________________________________ 4. In general, how many complaints in a single day have you, other members of your household, or guests make aloud when encountering the outdoors near your home:_________ 5. How often do you encounter mosquitoes indoors? ____Daily____A few times a week____Once a week ____Rarely____Never 13 Task 4. Your Data (Second week of each month)

14. Task 4. Your Data (1 day in the middle of each month, please use same time of day and same location) Instructions: Go outdoors when you think mosquito activity is high. Pick a location where you enjoy sitting. And complete the following. Nuisance Data Worksheet- Please Rate the Following Nuisance levels as appropriate: Day# __________, JuneTime of Day (circle one): Morning (6am-11am), Afternoon (12pm-6pm), Evening (6pm-11pm) Time to first encounter/first seeing a mosquito (indicate seconds or minutes): ________ Circle # of mosquitoes encountered: NONE, FEW (1-3), SOME (4-10), MANY (+ 10) How tolerable was your experience (circle one): JUST FINE, IRRITATING, INTOLERABLE Day# __________, JulyTime of Day (circle one): Morning (6am-11am), Afternoon (12pm-6pm), Evening (6pm-11pm) Time to first encounter/first seeing a mosquito (indicate seconds or minutes): ________ Circle # of mosquitoes encountered: NONE, FEW (1-3), SOME (4-10), MANY (+ 10) How tolerable was your experience (circle one): JUST FINE, IRRITATING, INTOLERABLE Day# __________, AugustTime of Day (circle one): Morning (6am-11am), Afternoon (12pm-6pm), Evening (6pm-11pm) Time to first encounter/first seeing a mosquito(indicate seconds or minutes): ________ Circle # of mosquitoes encountered: NONE, FEW (1-3), SOME (4-10), MANY (+ 10) How tolerable was your experience (circle one): JUST FINE, IRRITATING, INTOLERABLE 14