1. Extracting Biologically active substances from Plants Haruna Kobayashi Yohei Nosaka Yuta Fukushima Eriko Minamiya Koshi High School

2. We listened to our seniors’ study “Making chemical-free fertilizer with plant components” and we will extract component from plants to find good applications for our life. ~Motive~

3.  Cinnamon  Cork Tree  Bracket fungus  Chaga  Rooibos ~Research Material~

4. ・ Spice ・ We say it has hyperthermic action. Cinnamon Cork tree ・ Rutaceae Rooibos ・ we say it is a medicine of perpetual youth and long life ・ It has antioxidant action

5. ・ Shelf-type mushrooms ・ It is born in the stem of natural trees, fallen trees, and dead trees Bracket fugus ・ Cold-resistant mushroom ・ It is born in cold areas Chaga

6. Bracket fugus and Chaga are relatives. But both of them are different in trees. Do they have different components!?

7. Crush material with ethanol using a machine. Leave it for three weeks. Concentrate it. Add water to it, to get n-Hexane Extracts. ～ First Experiment ～

8. ① Make 10ml of solution which is 90% Chloroform and 10% Methanol (we will just call it “the solution” after this), and make a sample with the solution. ～ Method ～ Vacuum Filtration Concentration ～ Thin Layer Chromatography(TLC) ～

9. ② Spot the bottom of the TLC plate with the concentrate from step ①. Then, we put 5ml of the solution into a glass case and we put the TLC into the case.

10. ③ After the solution rises enough, check if you can see component spots with UV light.

11. … Spots of components （ A thicker mark means the component is more concentrated. ） … The first spot ～ Results of Experiment ～ Ⅰ Cinnamon Ⅱ Rooibos Ⅲ Bracket fugus Ⅳ Cork tree, Chaga The line of rising solution

12. ～ Column Chromatography ～ ① Make 200ml of the solution ② Put Silica gel and the solution into an Erlenmeyer flask and mix them. ～ Method ～～ Method ～ Chloroform Methanol Silica gel

13. ③ Put ② into a column and pour in a little solution. After that we put 5ml of the solution mixed with a sample into the Column.

14. ④ Put a little Silica gel on ③ and pour little by little another 200ml of the solution. → Silica gel → Sample → Solution → Expand Mixed Silica gel and 5ml solution

15. ⑤ Divide the liquid into 2 equal parts, put in a Flask and concentrate them.

16. Result of Experiment ColorFragrance C－０C－０ strong red C－１C－１ YellowWeak C-0 smell C－２C－２ YellowStrong C-0 smell C－３C－３ Reddish brown Ⅰ :Cinnamon

17. Ⅱ :Rooibos Ｒ－ ０ Ｒ－ １ Ｒ－２Ｒ－２ ColorFragrance R－０R－０ Strong yellow-green R－１R－１ Weak yellow-green R－２R－２ Weak Yellow-green

18. Ⅲ： Bracket fungus Ｓ－ １ Ｓ－ ２ Ｓ－ ３ Ｓ－ ４ Ｓ－ ５ ※ There is not S-0 picture. ColorFlagrance S－０S－０ Yellow-green S－１S－１ Strong yellow S－２S－２ Weak yellowS-0 smell S－３S－３ Weak yellowSour smell S－４S－４ Weak yellowSour smell S－５S－５ Weak yellow

19. ⅠⅡⅢ … Spot of components （ a thicker mark means the spot is more concentrated ） …… The first spot C －１ C －２ C －３ R－１R－１ R－２R－２ C －０ R －０ S －１ S－０,S－０, ２～５

20. ～ Consideration ～ ・ C-2 has a strong C-0 smell ⇒ We think C-2 has fragrance components. ・ C-3 has C-0 color ⇒ We think C-3 has color components. ・ R-2 is like C-0 color ⇒ We think R-2 has color components. Cinnamon Rooibos Bracket fugus ・ S-2 is like S-0 smell ⇒ We think S-2 has fragrance components.

21. ～ Future Activities ～ ・ Try to do other experiments. ・ Try to extract component of other plants. ・ Conduct experiments to test the effects of components. We will examine whether Rooibos have inhibits UV light and so on.

22. Thank you f r listening listening o