1. Staph and Strep

2. Cocci Morphology

3. Catalase Test Enzyme Detoxify hydrogen peroxide H2O2  H2O + O2
(+) bubbles
Staphylococcus
(-) no bubbles
Streptococcus
Enterococcus
Detoxify hydrogen peroxide
H2O2  H2O + O2

4. Columbia - CNA Selective for GP Differential Colistin Nalidixic Acid
Blood hemolysis

5. BAP: Hemolysis
Alpha
Gamma

6. BAP Hemolysis: Beta

7. Gram Stain: Staph

8. MSA Selective Differential 7.5 % NaCl, halotolerant Staphylococci
pH indicator: phenol red
Mannitol
Fermentation
Acid end products
Yellow
Non fermentation = red

9. MSA

10. DNase Differential Dye Enzyme Results Methyl green pH 7.5 hydrolytic
Clear zone = (+)

11. Mueller-Hinton Agar (MH)
AB sensitivity
Novobiocin Disc
Sensitive > 18 mm
Resistant < 18 mm
Staph chart for ID

12. Staph Tri-plates
Mannitol
Growth NF
DNAse (+)
Novobiocin (S)

13. Staph Tri-Plates
Mannitol
Growth
Fermentation
DNAse (+)
Novobiocin (S)

14. Staph Tri-plate
Mannitol
Growth NF
DNAse (-)
Novobiocin (R)

15. Staph Tri-plate
Mannitol
Growth
Fermentation
DNAse (-)
Novobiocin (R)

16. Coagulase Test Staph aureus Converts fibrinogen to fibrin
Immune defense
Protection from phagocytosis

17. Rapid Staph Test Staph aureus Virulence Factors Results Coagulase (+)
Fibrinogen  Fibrin
Clots
Protein A
Fc regions of IgG
Inhibits Ab
Results
Color Change
Clumping

18. Anaerobic Systems
Facultative Anaerobes
Staph
Capnophiles
Strep

19. Streptococcus GP cocci Chains Catalase (-) Capnophiles Groups
Lancefield Groups
Surface Antigens

20. Lancefield Strep Classifications
Group A
S. pyogenes
Group B
S agalactia
Group C
S. equi
Group D
Enterococcus faecalis
S. bovis
Group K
S. salivarius
Viridans
S. mutans
S. mitis
Pneumoocci
S. pneumoniae

21. Catalase and MSA Catalase (-) MSA No growth Except Not halotolerant
Enterococcus
(-)

22. Hemolysis Types

23. Bacitracin and SXT Sensitivities

24. Zone of Inhibition

25. Ab Sensitivities Bacitracin SXT Any zone = Sensitive
No clearing = Resistant
SXT
Zone > 20 mm = S
Zone < 20 mm = R

26. Bile Esculin Selective Differential Results Bile salts
Enteric organism growth
Differential
Hydrolyze esculin (CH2O)
Reacts with FeNH3-citrate
Results
Growth
Black precipitate

27. CAMP Identify Group B Strep (GBS) CAMP factor enhances hemolysis
Arrow Head produced for GBS

28. Rapid Strep Test Group A Strep Immunoassay Results Ag ID
Ab on test strip
Results
Color change

29. Flow Chart