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Chapter 2 – Chromosomes and Sexual Reproduction
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Basic Cell Types - Prokaryotic “before nucleus” Unicellular Simple structure –No internal membranes Eubacteria –“true bacteria” Archaea –“ancient bacteria” –More closely related to eukaryotes
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Basic Cell Types - Eukaryotic “true nucleus” Unicellular or mutlicellular Large, complex –Have internal membranes
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Genetic Material Prokaryotes –Single, circular chromosome May have small, accessory plasmids –Eubacteria DNA within cytoplasm (nucleoid region) –Archaea DNA may have associated protein (histones), but different from eukaryotic chromosomes Eukaryotes –Multiple, linear chromosomes –DNA with associated histone proteins Chromatin Chromatin arranged in specific complex to form chromosomes –Allows for packaging within a small nucleus
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Genetic Material - Viruses Protein coat surrounding nucleic acid (DNA or RNA) Not classified as living organism –Dependent on host cell for reproduction –Evolved after cells –Closely related to host Similar genes
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Cell Reproduction - Prokaryotic Binary fission Origin of replication –Initiation site of DNA replication 2 DNA molecules move to opposite ends of cells –Proteins bind near replication origins and anchor new DNA molecules to plasma membrane
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Cell Reproduction - Prokaryotic New cell wall forms to produce 2 cells Identical to each other, and parent cell Asexual reproduction
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Cell Reproduction - Eukaryotic Chromosomes –Each species has a characteristic number –Diploid cell/organism Has 2 copies of each chromosome Homologous chromosomes –Save same genes at same locus –May have different alleles –Haploid cell/organism One set/one copy of each chromosome
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Chromosome Structure 3 components of a functional chromosome –Centromere Attachment point for microtubules Kinetochore – protein complex attached –Telomere Ends of linear chromosomes stabilize –Origins of replication 2 identical copies of DNA = sister chromatids –Held together by common centromere
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Chromosome Classification Classified by location of centromere “p” arm and “q” arm Humans do not have telocentric chromosomes
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Cell Cycle Interphase –Extended period of growth Mitotic phase –Mitosis –Cytokinesis Key areas are regulated at checkpoints
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Interphase G 1 S G 2
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Mitosis Nuclear Division 5 stages –Prophase –Prometaphase
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Mitosis 5 stages con’t –Metaphase –Anaphase
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Mitosis 5 stages cont –Telophase
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Movement of Microtubules
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Cytokinesis Division of cytoplasm –Animal cells – cleavage furrow –Plant cells – cell plate Usually occurs simultaneously with telophase End result of mitosis/cytokinesis is 2 identical cells –Asexual reproduction
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Sexual Reproduction and Genetic Variation Meiosis –Creates gametes/sex cells/egg and sperm –Chromosome number is reduced by half Diploid to haploid Fertilization –Fusion of egg and sperm to restore diploid condition Forms diploid, single cell - zygote –Sexual reproduction 1 or 2 parents
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Meiosis One DNA replication followed by two rounds of division Meiosis I –Reduces number of chromosomes Diploid to haploid Meiosis II –Separates sister chromatids
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Meiosis I Prophase
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Meiosis I Metaphase Anaphase
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Meiosis I Telophase Interkinesis –Nuclear envelopes are re- formed and spindle breaks down Some cells skip to metaphase II –Cell may or may not split into two cells
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Meiosis II Prophase Metaphase
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Meiosis II Anaphase Telophase
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Consequences of Meiosis End result is 4 haploid cells from one diploid cell –Each is genetically different Crossing over –Exchange between homologous chromosomes –Sister chromatids are not identical Random assortment –Shuffles maternal and paternal chromosomes in different combinations –Metaphase I
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Crossing Over and Random Assortment
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Separation during Mitosis Cohesin holds sister chromatids together –Established during S –Broken down during anaphase by separase Separase is inactive during Interphase and early mitosis
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Separation during Meiosis Cohesin aids in formation of synaptonemal complex Anaphase I –Cohesin broken down by separase –Centromeric cohesin is protected by shugoshin Keeps sister chromatids together Metaphase II –Separase breaks down shugoshin Allows separation of sister chromatids
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Meiosis in Animals
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Alternation of Generations in Plants
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