1. Start date: September 2010 Duration: 36 months Website: www.mycohunt.euwww.mycohunt.eu Funding Scheme: FP7 Research for the Benefit of SME-AGs The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 286522. 1

2. ORGANIZATION NAMECOUNTRY Hungarian Grain and Feed Association Greek Associations of Traders and Exporters of Cereal By-products and Feeds Confederacion Espanola de Fabricantes de Alimentos Compuestos para Animales Associación Espanola de Fabricantes de Masas Congeladas Synagra Impuls OSV Dunagabona Dimitriaki Hogeschool Ghent Universiteit Ghent Union of Agricultural Cooperatives of Rethymo Evaluation technologique, Ingénierie et Applications BIOFORUM EST Hungary Greece Spain Belgium Poland Italy Hungary Greece Belgium Greece France Greece UK 2

3. TECHNOLOGICAL OBJECTIVES To develop effective, non-destructive sampling apparatus in order to assure adequately representative measurement aiming at 90% correlation with the bulk samples. To assess detection limit of 50 ppb, efficacy, specificity and sensitivity of the sensor To develop a data evaluation algorithms and to set up correlation databases To develop the ‘plug & play’ disposable cartridge to house the antibody-binding assay SCIENTIFIC OBJECTIVES The understanding and determination of parameters (temperature, pressure, vacuum, etc) affecting the sampling precision avoiding the damaging of grain. To develop immobilising methods for sensitive antibodies, and assess different approaches to enhance sensitivity, reproducibility, life-time, detection range, detection limit, and/or other specifications. The investigation of the cross-reactivity of other trichothecenes such as 3-acetyl-DON, 15-acetyl-DON, nivalenol, etc. ECONOMIC OBJECTIVE To realize a commercial price for the sensor while keeping the price of the whole apparatus low. TRAINING, DISSEMINATION AND EXPLOITATION OBJECTIVES The development of training activities addressed to the SME members of the SME-AGs, first in pre-validation stage in order to ensure the understanding of the technology operation before the partners initiate the validation tasks. Technology transfer will be carried out to present the project results. To develop a sound dissemination plan to deliver at least 4 workshops, presenting the results of the project in European conferences and specialised magazines 3

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5. 5 The MYCOHUNT system was fully designed reflecting the content of the system specification with the assistance of SME Association and SME partners. The MYCOHUNT concept of testing DON from wheat dust has been proven via defining the correlation between DON found in wheat grain samples and in wheat dust samples. DON selective antibodies have been developed to be used in the immunosensor for DON detection. Assay development and characterization for the immunosensor is under progress. The components of the prototype have been constructed. The physical realization and the integration of the complete system including the sampling unit, the sensor unit and their control system are under development and will be installed at the end-user facilities to continue with the prototype demonstration.

6. Name of Partner www.mycohunt.eu mycohunt@mfkk.hu (06 1) 787 4024 6