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Lactic Acid Bacteria Hopkins Microbiology Course 2010 Marcus Schicklberger
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Lactic Acid Bacteria Gram positive Non-sporforming Anaerobes, lack catalase Acid- and halotolerant Ferment glucose to only lactic acid or to lactic acid, CO 2 and EtOH Obtain energy only from the metabolism of sugars Have limited biosynthetic abilities complex media often plant- and animal-associated Don´t need iron advantage: no pathogens in niche Streptococcus pneumoniae: meningitis, pneumonia Lactobacillus acidophilus
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homofermentativeheterofermentative
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Lactic Acid Bacteria in foodproduction among the most improtant bacteria in food fermentation contribute to taste and texture of fermented products inhibit food spoilage bacteria by producing growth inhibiting substances and large amounts of lactic acid.
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pH initial = 6.0 pH final = 4.2 plate cabbage juice on glucose- CaCO 3 plates O 2 O 2 - H 2 O 2 H 2 O+O 2 e-e- e-e- catalase CaCO 2 Ca 2 + +CO 3 2- HCO 3 - H 2 CO 3 H 2 O+CO 2 H+H+ H+H+ Isolation of Lactic Acid Bacteria 3d @ 30°C
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