1. DEVELOPING OF INTEGRATED FARMING’S MODEL TO MAINTAIN THE GREEN BIOTECHNOLOGY NI NYOMAN TRI PUSPANINGSIH MIRNI LAMID ENDANG SRI MASITHAH AIRLANGGA UNIVERSITY : AUTONOMY UNIVERSITY, EXCELLENCE WITH MORALITY

3. ENZYME RESEARCH ON : PECTINASE HEMICELLULASES CELLULASE PLANT CELL WALL EXCELZYME

4. Why enzymes? Advantages Natural, Nontoxic Catalyze specific reactions Active under mild conditions Active at low concentrations Can control rate of reaction Can be inactivated

5. EXCELZYME TOWARDS GREEN TECHNOLOGY EXCELZYME 1: REDUCING OF 30-40% CHEMICAL COMPOUNDS BIO-DEINKING/BIO-BLEACHING ON PULP AND PAPER INDUSTRY EXCELZYME 2 : NUTRIENT ENHANCEMENT OF AGROINDUSTRIAL WASTE AS RUMINANTS FEED FORMULATING FEED ENZYME IN COMPLETE FEED

6. EXCELZYME 3 : REDUCED ENVIRONMENTAL IMPACT OPTIMIZE THE COMPOST PROCESS BASED ON LIGNOCELLULOSE MATERIAL AND ANIMAL WASTE FOR BIO-FERTILIZERS (ORGANIK AND PLANKTON FERTILIZERS) EXCELZYME 4 : REDUCED ENVIRONMENTAL IMPACT OPTIMIZE EXCELZYME 4 AS PREBIOTIC FOR REDUCING BLOOMING MICROCYSTIS EXCELZYME

7. Low Quality Lignocellulose Biomass Increasing Crops Excelzyme treatment Efficient Technology Agriculture and Fish Pond High Quality of Ruminant and Poultry Feed Organic and Plankton Fertilizers Source of animal protein Increasing of animal weight Animal Waste + Biomass Excelzyme treatment GL0BALWARMINGGL0BALWARMING INTEGRATED FARMING’S MODEL

8. Lignocellulose + Excellzyme3 Lignocellulose + Excellzyme3 + H2O2 A. Excelzyme 4 : Reducing Blooming Microsystis beforeAfter + Excelzyme 4 B. Excelzyme 3 : Indicating hemicelulase activity ANALYSIS EXCELZYME ACTIVITIES

9. ORGANIC FERTILIZERS EXCELZYME APPLICATION

10. FISHERIES APPLICATION BLOOMING MICROCYSTIS GROWING THE PLANKTON FERTILIZER 1.PLANKTON FERTILIZER 2.REDUCING BLOOMING MICROCYSTIS

11. ANIMAL FEED ENZYME USING RICE STRAW NUTRITION CONTENTSRICE STRAWRICE TRAW + EXCELZYME DRY MATERIAL (%)95,3992,37 CRUDE FIBER (%)33,8324,14 CRUDE PROTEIN (%)5,467,47 ASH ((%)20,4320,33 ORGANIC MATERIAL (%)74,9672,03

12. ENZYMES FOR DOMESTIC DEMAND Lignocellulosic waste : 169 million ton/year Agrobusiness Demand (ton/year) (in million) Enzymeton/year Average Price/kg (USD) import Animal feed 7.61.510-15 Fertilizer0.90.098-10 Pulp/Paper91.814-20

13. Acknowledgement International Research Group Collaboration on Lignocellulase Enzymes of Airlangga University with : 1.University of Groningen, The Netherlands 2.Mie University, Japan 3.Universiti Putra Malaysia, malaysia 4.Universiti Kebangsaan Malaysia, Malaysia 5.Universiti Teknologi Malaysia, Penang, Malaysia The activities : Joint Research, Joint Supervisors, Student Exchange, Joint Publication Collaboration with Indonesian industries : PT Bumiindo (Bio-energy) PT Pupuk Kaltim (Bio-Fertilizer) PT Siklus Alam Semesta (Bio-Fertilizer) PT Adi Prima (Paper and Pulp Industry) PT Eka Timur Raya (Bio-Fertilizer)

14. THANK YOU