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Published byElijah Taylor Modified over 9 years ago
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SSterile petri dish containing agar and usually nutrients UUsed to culture microorganisms
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AGARSREGULAR“BLOOD”“SUGAR”PREPOUREDADDITIVES SUSPEND IN H20
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AGARDEFINED Chemicals required UNDEFINED Natural products SELECTIVE Certain organisms PERMISSIVE Many organisms
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AAgar plates are used as a medium for growing microorganisms, usually bacteria AAgar plates contain a gelatin, this is because this substance usually isn't degraded by bacteria. This is why some plates contain different material UUsed to help make decisions with prescribing medications or just for identification WHY?
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Commonly used due to high number of microbes, fungus and bacteria that can be grown (not selective) Beef broth and extracts from yeast make it non selective Commonly used due to high number of microbes, fungus and bacteria that can be grown (not selective) Beef broth and extracts from yeast make it non selective NUTRIENT AGAR
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CContains 5% blood (sheep) CCan be contaminated from human contact Blood Agar
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EMB AGAR EEosin Methylene Blue IIsolates and differentiation of gram negative Bacilli OOne of the many agars that are irritating to the eyes, skin and respiratory system
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Most of agar is made of chocolate, 1% hemoglobin (sheep) Usually for Haemophilus growth, fastidious bacteria Can be contaminated by human contact CHOCOLATE AGAR
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Usually for growth of gram negative E.Coli grow into red colonies AGAR POWDER: Sugar Lactose Non Sugar MACCONKEY AGAR
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10g lactose 20g peptone 5g bile salts "pinch" or small amount of 1mg/mL Bromcresol purple or Crystal violet solution. 15g agar 1L distilled water
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KKills gram - and + CContains neomycin antibiotic, which is naturally made from Streptomyces fradiae RRelatively toxic but can cause allergic reactions in some people NEOMYCIN AGAR
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LB (Luria Bertani) Agar Non-nutrient Agar Sabouraud Agar Thayer-Martin Agar Tryptic Soy Agar XLD Agar Xylose lysine Deoxycholate Mannitol salt Potato dextrose Millers LB
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www.sciencebuddies.org/scienceproject/microbiology/ www.google.com/agar_plates
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