1. All origami shown in this report is from the same annealing run that Massud showed in his 6/21/2011 weekly report

2. biotin/streptavidin position on A tile of 2D DNA array (see next slide) staple:m13 5:1 [m13]=10 nm SA:biotin staple 15:1 this annealing run was not dialyzed before adding SA and imaging

3. 58.88nm Two biotin on each side of A tile SA labeled A tile

4. SA labeled 2D cross DNA origami array

5. A subunit with total 4 biotin B subunit with no biotin + anneal over weekend A subunit with total 4 biotin B subunit with no biotin + 15:1 SA:biotin staples AB array XAB array YSAAFM #1 (mica) AB array X AB array YSA + + 66nM SA (15:1) anneal over weekend + 4 deg C overnight AB array XSAAB array YSASA AFM #2 (mica) All AFM images shown in final 6 slides of this presentation were previously shown in group meeting by Massud and are of AB array XSA below is a flowchart type key to the AFM images in the subsequent slides, any images that are not square can be assumed to have long side (X) length equal to the Y range seen on side of image. Unless otherwise stated, all images on a single.ppt slide are of the very SAME mica surface/depostion. Also, unless stated, deposition was by 5 min. incubation of origami soln. on mica followed by single HOH rinse.

6. AB array YSAAFM #1first mica after one HOH rinse

7. AB array YSAAFM #1same mica after second HOH rinse

8. AB array YSASAAFM #2first mica after one HOH rinse

9. AB array YSASAAFM #2second mica one HOH rinse

10. AB array XAFM #1

11. AB array XSAAFM #2 this is the first piece of mica imaged, TAE rinsed at left, TAE rinse followed by HOH rinse below

12. AB array XSAAFM #2second piece mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; continued next slide

13. same mica surface/ deposition as seen in previous slide

14. AB array XSAAFM #2 second peice mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; the only difference between this slide and the 2 preceding slides is that the same mica surface seen in previous 2 slides, sat in covered dish in ambient conditions for 6 days prior to acquisition of the images on this slide

15. Massud included the following slides in his 6-21-11 presentation

16. SA labeled 2D cross DNA origami array (this and all slides following are of AB array XSA)

17. SA labeled 2D cross DNA origami array