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Kingdom of Saudi Arabia King Saud University College of Applied Medical Sciences Biomedical Technology Department
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By: Badr AlShamary & Mohannad Abuljadayel Supervised By: Mr. Mohammed Nisar
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Polymerase Chain Reaction molecular biological method for amplifying DNA without using a living organism. PCR is commonly used in: Identification of genetic fingerprints. Diagnosis of infectious diseases. Diagnosis of virus diseases.
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A T G CA TC G The four bases found in DNA are Adenine (A), Cytosine (C), Guanine (G) and Thymine (T).
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Thermocycler
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Temperature Graph
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Sample Contents DNA original Sample Primers DNA Polymerase Buffer Solution Nucleotides(A,T,C and G) Primers DNA Polymerase Buffer Solution Nucleotides
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PCR Process Step1: Denaturation
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PCR Process Step2: Annealing
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PCR Process Step3: Extension
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PCR Process Doubling DNA each Cycle (Cycle:2)
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PCR Process (Cycle: 3) Number of copies= 3 2 = 8
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Virus Diagnosis By PCR Human DNA Virus DNA
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Human DNA Virus Diagnosis By PCR Virus DNA
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Human DNA Virus Diagnosis By PCR Virus DNA
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Thank You
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