1. QuattroMix™ Single Use Mixing System For Single Use Bag Integration work In cooperation with:

2. QuattroMix Principles Application: Design 16 outlet nozzles Single Use Bag Theoretical fluid stream Connected with pump in- and outlet Exocirculation™ Via pump Random Vector™ No Vortexing

3. Performance test 500L Solid/liquid mixing performance: Addition of NaCl to 500L Water 4g/l Completely dissolved after 10 min 2g/l; Completely dissolved after 5 min 500L bag with two QuattroMixers, in parallel connected to one QF1200SU.

4. Applications Typical application in biopharmaceutical processes Buffer preparation Media preparation Virus inactivation And others Salts Purified Water Acids/ Bases Carbohydrates Nutrients SU Bag with Mixer Sterile Filtration Upstream or Downstream Process

5. QuattroMix Enables: Quattroflow Pump becomes a multi purpose device, because –Recirculation of liquid is used for mixing effect –Can be used for filling and emptying of the bag –Can be used for post-mixing processes, like sterile filtration, virus filtration, flow through chromatography, etc. Recirculation loop allows for additional features like –Integration of in-line sensors (pH, conductivity, temperature, UV) –Usage of heat exchanger for cooling/heating of media Filter Filtrated Product Heat exchanger TIR Feedstock

6. JetMixer Solid/liquid mixing performance: Addition of 500 g NaCl to 200L Water Result: Better performance of QF mixer Still undissolved NaCl after 18 minCompletely dissolved after 4 min Comparison 200L

7. Performance test JetMixer Liquid/liquid mixing performance: adding 100 g predesolved NaCl to 200L Water Result: Comparable mixing performance:

8. Comparison 200L JetMixer QuattroMix

9. Available For: Application development work Mixing problem resolution Bag Sizes: 100L, 200L with single mixer 500L with dual mixer Bag Configuration/porting On request to HPNE From: HPNE