1. GURU BRAHMA GURU VISHNU GURU DEVO MAHESWARAHA GURU SHATHSHATH
DR.P.K.RATH MD(PATH)
GURU BRAHMA
GURU VISHNU
GURU DEVO
MAHESWARAHA
GURU SHATHSHATH
PRA BRAMMAM
DHASMAI
SREE GURUVE
NAMAHA

2. QUALITY ASSURANCE IN BLOOD BANKING
Aravindakshan. MURALIDHARAN
Doctors Blood Bank & Research Centre
Trichy

3. INTRODUCTION
WHO defines the term QUALITY as, “ The consistent and reliable performance of Services or product in conformity with specified standards”
QUALITY ASSURANCE is vital for the successful operations of any blood transfusion services.

4. The All Important - Blood Donor - OUR DEMIGOD
Donor Motivation
Donor Awareness
Donor Incentive

5. Donor Selection
The Single most important factor towards Our Final Goal
SAFE BLOOD
Donor Experience
Donor Retention
Adherence to the SOPs

6. Blood Collection Blood Bank policy regarding the blood usage
Choice of Bags : Quality of bag/ Type of Bag, Examination of Bags.
Good phlebotomy procedures
Blood Donor recovery and refreshment

7. Blood Component Preparation :-
SOPs as per NABH, DGHS Guidelines for the preparation of Red Cells, FFP, Platelets & Cryo.
Instrument calibration and validation

8. Storage
Calibration and validation of various equipments involved in storage such as BBRs, Deep Freezers, Platelet incubator and agitator etc.

9. Q A in Screening of Blood
Grouping : Manual / Automated
TTI Screening : Manual / Automated
Quality control in this with positive control, negative controls, L J chart in this will help.

10. QC of Blood Component Preparation
Whole blood :
Volume : 350mls excluding anticoagulant
HCT : 40±5%
pH > 6.5
K < 27mmol/L
Sterility : no growth

11. Red Cell Concentrates The expected values
Volume : 280ml ± 50ml, frequency of control 1% of all units
HCT : %
pH > 6.5
K < 78 mmol/L
Sterility : no growth

12. Platelet Concentrates :
Volume > 30ml
pH :
Plt count : at least 5.5 x 1010 /bag in at least 75% of the units tested at the end of the storage.
By apheresis : minimum 3 x 1011/bag platelets in at least 75% units tested
WBC contamination: < 2 x 103/bag.
RBC contamination: minimal
Macroscopic appearance : no visible platelets aggregates
Sterility : no growth

13. Fresh frozen plasma
Quality Control
Volume: ml
Should contain necessary units of factor VIII, factor IX, vWF and other plasma clotting factors
Macroscopic Examination: no abnormal color or visible clots

14. Cryo precipitate Quality Control : Volume : 10- 15 ml
Factor VIII : 80 – 100 units/ Conc.
Fibrinogen : 150 – 250mg/ Conc.
von-Willebrand factor :40–70% of the original
Fibronectin : 55 mg
Factor XIII: 20-30% of the original
Sterility: no growth
References : Compendium of Transfusion Medicine – Dr. Makroo

15. Issue of Blood Products:
SOPs for issue of individual products
Thawing procedures and calibration for thawing bath
Packing and transportation of the products

16. QC FOR EQUIPMENT

20. Trouble shooting of untoward incidences
Reporting and recording of untoward incidences
Recall of components and rechecking
(separate SOP for this should maintain)
Changes in SOP for prevention of such incidences

21. TO CONCLUDE Donor Selection and Retention Instrument calibration
Test Validation
Maintanence of periodic QC of various blood products
Strict adherence to SOPs
Good Laboratory Practices - GLP

22. Quality Assurance is the right of every patient and it is the Duty of Every Blood Centre.

23. References: Compendium of Transfusion Medicine by
Dr. R.N, Makroo MBBS, DIBT, MD
Second Edition
Transfusion Medicine Technical Manual by Director General of Health Services, INDIA - Second Edition 2003

24. Thank you