1. DISCREPANCY IN NUCLEAR, MITOCHONDRIAL AND KARYOLOGICAL CHARACTERISTICS OF MUS MUSCULUS SUBSPECIES IN PRIMORYE HYBRID ZONE IN PRIMORYE HYBRID ZONE Spiridonova L.N. Institute of Biology and Soil Science, Far East Branch, Russian Academy of Sciences, Vladivostok 690022, Russia; spiridonova@biosoil.ruspiridonova@biosoil.ru

2. The aim of this investigations is: Determination of congruence between taxon-specific markers on different inheritance systems (n-, mt-DNA and chromosomes) for revealing the pattern of real genetic structure of M. musculus in the extensive hybridization conditions in Primorye Determination of congruence between taxon-specific markers on different inheritance systems (n-, mt-DNA and chromosomes) for revealing the pattern of real genetic structure of M. musculus in the extensive hybridization conditions in Primorye

3. The tasks of investigation are: To study subspecific structure of wild mice on nuclear DNA level in Primorye hybrid zone with RAPD analysis; To study subspecific structure of wild mice on nuclear DNA level in Primorye hybrid zone with RAPD analysis; To sequence control region of mtDNA and identify subspecific haplotypes; To sequence control region of mtDNA and identify subspecific haplotypes; To compare molecular and karyological data for clear up real genetic structure of house mouse populations. To compare molecular and karyological data for clear up real genetic structure of house mouse populations.

4. 76 samples from 9 places of Primorye were used as the research material The wild mice were collected by colleagues of our lab (Korobitsyna К.V., Frisman L.V., Yakimenko L.V., Korablev V.P., Pavlenko M.V., Chelomina G.N.) and me. The map of the collecting grounds

5. The primers used for studying

6. VladivostokKraskino KhasanTury rog 1200 bp 1012 bp RAPD-bands of M. musculus total DNA amplified with primer OPC-07 11317 musculuscastaneusdomesticus mus cas dom bac 1 - protein coding gene Aff3 13 - gene GAS 1 (growth arrest specific 1) 17 - gene which is coding unknown hypothetic protein similar to retinitis pigmentosa

7. m u s c u l u s castaneus domesticus outgroup Phylogenetic tree constructed by ME method for M. musculus subspecies from sequences of mtDNA control region made with MEGA 4

8. The presence of M. musculus subspecific nuclear, mitochondrial DNA and chromosomes in Primorye mus* - own results, (R) – tandem repeat of control region segment from 67 to 81-bp mus (-) – from Yonekawa et al., 2003 ** - from Yakimenko et al., 2000

9. The diagram of nuclear and mitochondrial subspecific characteristics of Mus musculus in Primorye domesticuscastaneusgansuensismusculus

10. 10, 14, 15,18 C± C-C- C-C- C-C- C-C- C± 17 C± 12, 15, 17, 18 C- 5, 17, 18, 19 C± cas (dom?) gan mus karyological data (Yakimenko et al., 2000) Legend of nuclear and mitochondrial data The compared map of nuclear, mitochondrial and karyological data in M. musculus from Primorye mtDNAnDNA

11. Conclusions: the inconsistency in markers distribution of different inheritance systems of wild mice was shown, that is the consequence of extensive introgressive hybridization in this region; the inconsistency in markers distribution of different inheritance systems of wild mice was shown, that is the consequence of extensive introgressive hybridization in this region; in most cases genetic diversity revealed by nDNA was higher than by mtDNA and chromosomes; in most cases genetic diversity revealed by nDNA was higher than by mtDNA and chromosomes; musculus mitochondrial haplotype (83%) predominates in all studied samples that fits to a pattern of absorption crossing; musculus mitochondrial haplotype (83%) predominates in all studied samples that fits to a pattern of absorption crossing; the tendency for conservation of subspecific features of M. musculus (61%) is observed in the nuclear genome. the tendency for conservation of subspecific features of M. musculus (61%) is observed in the nuclear genome.

13. Literature: А.И. Козловский, Н.Ш. Булатова, В.Н. Орлов «Неадекватность интерпритации результатов цитогенетического и биохимического анализа домовых мышей Туркменистана» 1997, ДАН А.И. Козловский, Н.Ш. Булатова, В.Н. Орлов «Неадекватность интерпритации результатов цитогенетического и биохимического анализа домовых мышей Туркменистана» 1997, ДАН H. De Wolf, T. Backeljau, R. Verhagen «Congruence between allozyme and RAPD data in assessing macrogeographical genetic variation the periwinkle Littorina striata (Mollusca, Gastropoda)» 1998, Heredity H. De Wolf, T. Backeljau, R. Verhagen «Congruence between allozyme and RAPD data in assessing macrogeographical genetic variation the periwinkle Littorina striata (Mollusca, Gastropoda)» 1998, Heredity I. GÜNDÜZ et. all. «Mitochondrial DNA and chromosomal studies of wild mice (Mus) from Turkey and Iran» 2000, Heredity I. GÜNDÜZ et. all. «Mitochondrial DNA and chromosomal studies of wild mice (Mus) from Turkey and Iran» 2000, Heredity Y.-H. Liu et. all. «Mosaic genealogy of the Mus musculus genome revealed by 21 nuclear genes from its three subspecies» 2008, Genes Genet. Syst. Y.-H. Liu et. all. «Mosaic genealogy of the Mus musculus genome revealed by 21 nuclear genes from its three subspecies» 2008, Genes Genet. Syst.