1. PROJECT LIFE12 ENV/IT/00036 “Anti-infective environmental friendly molecules against plant pathogenic bacteria for reducing Cu” “AFTER Cu” Start: 01/01/2014 End: 31/12/2015 11/06/2015 18 Month and monitoring meeting Firenze - Italy DISPAA premises

2. PROJECT LIFE12 ENV/IT/00036 AGENDA 18 MONTH AND MONITORING MEETING 9:30 [DISPAA] Welcome and opening of the meeting TECHNICAL MEETING 09:40 [All] Actions carried out by each partner and scheduled activities from January 2015 until the end of May 2015. 11:15 [All] Coffee break 11:30 [All] After-Cu Technical future issues:  [DISPAA] Technical activities scheduled in the next six months of the project  [All] Open discussion on actions to be carried out in the next six months of the project

3. PROJECT LIFE12 ENV/IT/00036 AGENDA 12 MONTH MEETING 12:00 [DISPAA] Management and dissemination issues, in particular: o [DISPAA] Dissemination activities o [DISPAA] Preparation of Final Report 13:00 Lunch ADMINISTRATIVE MEETING 14:30 [EC Monitor team] After-Cu administrative documents monitor 16:30 Tour of DISPAA laboratories 17:00 End of monitoring meeting

4. PROJECT LIFE12 ENV/IT/00036 After-Cu PROJECT GENERAL SUMMARY

5. PROJECT LIFE12 ENV/IT/00036

7. Technical Actions carried out by each partner in the last six months  B.2 (April 2014-March 2015): Demonstration of the qualitative and quantitative yields of conventional chemical synthesis and biotechnological synthesis of the anti-virulence peptides at laboratory scale  B.3 (April 2014-March 2015): Demonstration of the chemical and biological stability of anti-virulence peptides produced by conventional chemical and biotechnological synthesis  B.4 (July 2014-June 2015): Demonstration of Kilo-scale biotechnological synthesis of the anti-virulence peptides by recombinant technology  B.5 (July 2014-June 2015): Demonstration of the null toxicity profile of the anti-virulence peptides on model organisms and microorganisms  B.6 (October 2014-December 2015): Demonstration of the anti-virulence peptides in vivo performances at pilot scale level in field screenings  B.7 (October 2014-December 2015): Demonstration of the effectiveness of the anti-virulence peptides in the presence of contamination by copper compounds at pilot scale level in field screenings.

8. PROJECT LIFE12 ENV/IT/00036 Technical Actions carried out by each partner in the last six months  C.2 (April 2014-March 2015): Monitoring of the in vitro anti-virulence performances of peptides at laboratory level  C.3 (April 2014-March 2015): Monitoring of the absence of side effects for the anti-virulence peptides on common targets of any living organism at laboratory level  C.4 (July 2014-June 2015): Monitoring of the absence of a direct selection operated by the anti-virulence peptides towards the emergence of bacteria resistant to the anti-virulence peptides themselves at laboratory level  C.5 (October 2014-December 2015): Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings  C.6 (October 2014-December 2015): Monitoring of the absence of an indirect selection operated by the anti-virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings  C.7 (July 2015-December 2015): Monitoring of technical-socio-economic assessment of the After-Cu project.

9. PROJECT LIFE12 ENV/IT/00036 Presentations of:  DISPAA  CEBAS  ICCOMCNR  ASTRA  SOLDANO.

10. PROJECT LIFE12 ENV/IT/00036 [All] Open discussion on actions carried out.

11. PROJECT LIFE12 ENV/IT/00036 Dissemination actions D1 (July 2015-December 2015): Demonstration workshop in Italy D2 (July 2015-December 2015): Demonstration workshop in Spain D3 (January 2014-March 2014): Project website D4 (January 2014-March 2014): Notice boards D5 (September 2015-December 2015): Layman's report D6 (January 2014-December 2015): Diffusion material preparation D7 (January 2014-December 2015): Articles and press releases D8 (January 2014-December 2015): Networking D9 (April 2015-December 2015): After-Cu technology manual D10 (January 2014-December 2015): Demonstration workshop, seminars, conferences and other events D11 (January 2014-December 2015): Dissemination to Institutions and policy makers D12 (September 2015-December 2015): After-LIFE Communication Plan D13 (January 2014-December 2015): International fairs and other events D14 (January 2014-December 2015): Digital supports for international diffusion

12. PROJECT LIFE12 ENV/IT/00036

13. Dissemination actions carried out by each partner in the last six months D1 Demonstration workshop in Italy (November 2015) ASTRA will organise a demonstration workshop in November 2015

14. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D2 Demonstration workshop in Spain (November 2015) CEBASCSIC will organise a demonstration workshop in November 2015

15. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D3 Project website 01/02/2014 and continuous update until 31/12/2015 After-Cu website in English, Spanish and Italian: www.lifeaftercu.com www.lifeaftercu.com In the website:  Visit counter  Link to LIFE+  Link to each beneficiary website  Results update  News update  Coming up  Reserved area  Link with the Facebook page for event booking/registration Facebook page

16. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D6 Diffusion material preparation 01/02/2014 and continuous update until 31/12/2015 6,000 After-Cu brochures 3,000 After-Cu plastic leaflets 7 After-Cu tissue posters in Italian and English 3 Malaga event posters 1 After-Cu roll-up 3 project presentations (English, Italian and Spanish) 500 After-Cu copybooks 800 After-Cu pens 200 After-Cu cups 500 After-Cu pendrives

17. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D7 Articles and press releases 10/01/2014 and continuous update until 31/12/2015 6 articles directly related to After-Cu:  AGI  UNIFI website  TUTTOGREEN (insert of “La Stampa” newspaper)  Fresh Plaza  CHIMET  Ambiente BIO 4 indirect articles related to the participation of After-Cu to the CNR event (in local and web newspaper) 12 indirect articles related to the participation of After-Cu to the ASTRA and ENEA event on 22 January 2015 in local and web newspaper 3 articles (Italian, Chinese and English) in Platinum journal

18. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D8 Networking 25/03/2014 and continuous update until 31/12/2015 Cluster with PODEBA, BIOREM, SOREME, BIONAD, GREEN SITE, SOILPRO, RESAFE, SEKRET, CLEANSED, AIS LIFE, EVERGREEN, HORTISED (LIFE 10-11-12-13-14 projects) Specific meetings with photos with BIOREM and CLEANSED Networking contacts at the CNR LIFE event at Pisa on 25 th March 2014 Networking contacts at the UNIFI LIFE event at Firenze on 24 th October 2014 Networking contacts at the ASTRA LIFE event at Faenza on 22 th January 2015

19. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D10 Demonstration workshop, seminars, conferences and other events 25/03/2014 and continuous update until 31/12/2015 LIFE CNR event in CNR premises on 25 March 2014 University of Siena event about life, Siena 29 May 2014 Seminar in Facultad de Ciencias - Área de Genética of Universidad de Málaga, Malaga 14 July 2014 Scuola Nazionale Metodologie Analitiche e Bioanalitiche in Spettrometria di Massa, Parma, Campus Universitario, Parma- Italy, 26-30 May 2014 Corso avanzato sull'accoppiamento della spettrometria di massa con tecniche di separazione in fase liquida, Lucca, 23-26 June 2014 Erice course on Biophysics of channels and transporters, Erice 11-17 May 2014 Event in EEZ of CSIC in Granada (Spain) on 10 July 2014 BRIGTH 2014 AREA CNR September 26th 2014 UNIFI LIFE event at Firenze on 24 th October 2014 Italy-Kazakhstan collaboration project event in Kazakhstan on 14/11/2014 ASTRA LIFE event at Faenza on 22 th January 2015 Italy-Kazakhstan day event in Florence on 12/05/2015 Reggio Calabria LIFE workshop (10 July 2015)

20. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D11 Dissemination to Institutions and policy makers 25/03/2014 and continuous update until 31/12/2015 All the After-Cu beneficiaries are responsible for the organisation of specific communication activities in order to disseminate the project actions and results to Institutions and policy makers. The following preliminary contacts with institutions have been taken: o Councillor for the Environment of Pisa City - Italy o Environmental Department of Pisa Area - Italy o Environmental Department of Murcia Area - Spain o Senator of the Italian Government - Italy o Assessorato Agricoltura Regione Emilia-Romagna – Italy o ENEA Manager Italy workshop (November 2015) Spain workshop (November 2015) Reggio Calabria LIFE workshop (10 July 2015)

21. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D13 International fairs and other events 28/07/2014 and continuous update until 31/12/2015 International Congress on Phytoremediation of Polluted Soils. Vigo, July 28 – 29, 2014 14th International Conference, Na,K-ATPase and related transport ATPases: Structure, mechanism, cell biology, health and disease. 30 August - 5 September 2014, The Netherlands XX National Congress Italian Society for Plant Pathology (SIPaV) Pisa, 22-23-24 Settembre 2014 "Environmentally loyal plant protection: from nano- to field-scale“ Conference Psyringae 2015, Málaga, 2-5 June 2015

22. PROJECT LIFE12 ENV/IT/00036 Dissemination actions carried out by each partner in the last six months D14 Digital supports for international diffusion 01/09/2015 and continuous update until 31/12/2015 Each beneficiary started to define the specific video of each beneficiary activity DISPAA is starting to have some contacts with some expert video companies in order to organise the general structure of the video DISPAA is starting to work on the video text

23. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  ACTION E.2: Monitoring  DISPAA has continuous contact with all project partners for monitoring project activities  Before the month end DISPAA receives from each beneficiary technical inputs for the monitor month summary  At the end of each month DISPAA prepares and sends a summary of the project activities carried out to monitoring team at the end of each month  Definition of management structures  Definition of payment procedures of each beneficiary  DISPAA organised the kick-off and progress meetings in scheduled time

24. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  ACTION E.2: Monitoring  DISPAA defined and sent the Partnership Agreement (following the EC scheme) and collected all the beneficiaries signatures  DISPAA uses project progress indicators for monitoring project activities  DISPAA has revised the project progress indicators in each of the projects coordination meeting in order to check any irregularities  DISPAA is checking the number and type of people reached by each dissemination activity in order to check if the diffusion actions have met their goals

25. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  Definition of management structures  Management and technical committe DISPAA: Stefania Tegli ASTRA: Vanni Tisselli SOLDANO: Maurizio Soldano CEBASCSIC: Carlos Izquierdo ICCOMCNR: Emilia Bramanti  Administrative committe DISPAA: Silvia Borselli ASTRA: Elisabetta Baldassarri SOLDANO: Domenica Timpano CEBASCSIC: Maria Teresa Hernandez ICCOMCNR: Manuela Cempini

26. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  Definition of payment procedures of each beneficiary  Each beneficiary has a specific payment responsible DISPAA: Stefania Tegli selects the project cost formally approved by department director or DISPAA council ASTRA: Vanni Tisselli as director selects and decides till an amount of € 20.000. If the cost are bigger than 20.000€ the decisions are assumed in accordance with Legal Representative. SOLDANO: Maurizio Soldano as manager selects and decides CEBASCSIC: Carlos Izquierdo selects the project cost formally approved by department director ICCOMCNR: Emilia Bramanti selects the project cost formally approved by department director

27. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  Definition of project accounting system and cost center All beneficiaries have defined the following internal specific code (codice commessa) which identify the project and all costs and income related to the project: DISPAA: B18C13001830006 ASTRA: X02R14 AFTER-CU LIFE12 CEBAS: 201920 ICCOMCNR: B18C13001830006 SOLDANO: After-CU For DISPAA, CEBAS and ICCOMCNR VAT is a cost.

28. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  Definition of project accounting system and cost center All beneficiaries respects the procedure of the best value for money for selecting all the project external and consumable costs:  DISPAA, CEBAS and ICCOMCNR: public tender through MEPA  ASTRA and SOLDANO: historical supplier or market analysis All the beneficiaries approved only the costs:  directly linked to, and necessary for, carrying out the After-Cu project;  reasonable, justified and comply with the principles of sound financial management, in particular in terms of economy and efficiency;  compliant with applicable tax and social legislation; and  actually incurred during the lifetime of the project, as defined in the grant agreement, and which could be identifiable and verifiable

29. PROJECT LIFE12 ENV/IT/00036 After-Cu Technical future issues for the next six months  B.4 (July 2014-June 2015): Demonstration of Kilo-scale biotechnological synthesis of the anti-virulence peptides by recombinant technology  B.5 (July 2014-June 2015): Demonstration of the null toxicity profile of the anti-virulence peptides on model organisms and microorganisms  B.6 (October 2014-December 2015): Demonstration of the anti- virulence peptides in vivo performances at pilot scale level in field screenings  B.7 (October 2014-December 2015): Demonstration of the effectiveness of the anti-virulence peptides in the presence of contamination by copper compounds at pilot scale level in field screenings

30. PROJECT LIFE12 ENV/IT/00036 After-Cu Technical future issues for the next six months  C.4 (July 2014-June 2015): Monitoring of the absence of a direct selection operated by the anti-virulence peptides towards the emergence of bacteria resistant to the anti-virulence peptides themselves at laboratory level  C.5 (October 2014-December 2015): Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings  C.6 (October 2014-December 2015): Monitoring of the absence of an indirect selection operated by the anti-virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings  C.7 (July 2015-December 2015): Monitoring of technical-socio- economic assessment of the After-Cu project

31. PROJECT LIFE12 ENV/IT/00036 After-Cu Technical future issues for the next six months [All] Open discussion on actions to be carried out in the next six months of the project

32. PROJECT LIFE12 ENV/IT/00036 After-Cu Dissemination future issues for the next six months  D1 Workshop in Italy: OK November 2015  D2 Workshop in Spain: OK November 2015  D3 Project website: update  D6 Diffusion material preparation: OK (foreseen 25 Posters, 10,000 leaflets/brochures/factsheets, 2,500 various branded items, now 9,000 brochures/leaflets, 10 posters, 1 roll-up, 3 presentations, 2,000 gadgets)  D7 Press and media releases: OK (foreseen 30 articles, now 25)  D8 Networking: OK OK (foreseen 10 projects, now 12)  D9 After-Cu technology manual: to be started (foreseen 1 manual)  D10 Demonstration workshop, seminars, conferences and other events: OK OK (foreseen 4 events, now 13 events)  D11 Dissemination to Institutions and policy makers: OK OK (foreseen successful communication, now 10 policy makers meetings)  D12 After-LIFE Communication Plan: to be started (foreseen 1 report)  D13 International fairs and other events: OK OK (foreseen 4 fairs, now 4 events)  D14 Digital supports for international diffusion: start (foreseen project video)

33. PROJECT LIFE12 ENV/IT/00036 ADMINISTRATIVE ISSUES  Final Report 31/03/2016  Next Deliverables  Partner costs May 2015  Daily rate analysis  Next meeting

34. PROJECT LIFE12 ENV/IT/00036 FINAL REPORT DISPAA will be responsible of the production of the Final report which will be made up by:  Progress report with the summary of technical, administrative and dissemination project issues  Dissemination Annex  Deliverables  Output indicators  Financial forms with all the originals signatures from each partner  Audit document The EC ok of the Final report will produce the project final payment from the EC

35. PROJECT LIFE12 ENV/IT/00036 FINAL REPORT  Documents needed from each beneficiary  DISPAA will check all the partner financial documents for final original versions with signatures from each partner DISPAA will be responsible for the production of an audit document signed by an external authorised auditor (already selected) which will certify all the project financial data. The selected auditor will check all the partner financial data and will be free to request to each partner more detailed documents.

36. PROJECT LIFE12 ENV/IT/00036 NEXT DELIVERABLES All the following Deliverables will be annexes of the Final Report :  Demonstration of the kilo-scale biotechnological synthesis of the anti- virulence peptides by recombinant technology: Action B.4  Demonstration of the anti-virulence peptides in vivo performances at pilot scale level in field screenings: Action B.6  Demonstration of the effectiveness of the anti-virulence peptides in the presence of contamination by copper compounds at pilot scale level in field screenings: Action B.7  Monitoring of the absence of a direct selection operated by the anti- virulence peptides towards the emergence of bacteria resistant to the anti- virulence peptides themselves at laboratory level: Action C.4  Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings: Action C.5

37. PROJECT LIFE12 ENV/IT/00036 NEXT DELIVERABLES All the following Deliverables will be annexes of the Final Report :  Monitoring of the absence of an indirect selection operated by the anti- virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings: Action C.6  Monitoring of technical-economic viability of the After-Cu project: Action C.7  After-Cu technology manual: Action D.9  After-LIFE Communication Plan: Action D.12  Layman’s Report: Action D.5

38. PROJECT LIFE12 ENV/IT/00036 Partner costs May 2015 PARTNERCLAIM Personnel Personnel May 2015Travel Travel May 2015 External assistanc e External assistance May 2015 Consuma bles Consumabl es May 2015 Other costs Other costs May 2015 Overhea ds Overhead s May 2015TOTAL TOTAL May 2015 DISPAA 205.000 185.550,10 12.500 3.688,14 88.300 41.161,48 105.000 32.862,94 - 30.000 18.428,39 440.800 281.691,05 ASTRA 137.500 70.091,92 12.500 2.155,55 27.800 16.650,00 45.000 5.675,38 - 247,64 16.000 6.637,43 238.800 101.457,92 Soldano 101.500 20.963,32 12.500 22.800 15.000,00 45.000 9.584,18 - 11.930 3.188,33 193.730 48.735,83 ICCOMCN R 52.000 44.262,94 12.500 1.035,04 13.200 9.999,97 25.000 12.090,49 - 7.000 4.717,19 109.700 72.105,63 CEBAS 155.000 74.090,80 12.500 6.484,86 18.400 4.097,76 95.000 66.393,29 - 19.000 10.574,67 299.900 161.641,38 TOTAL 651.000 394.959,08 62.500 13.363,59 170.500 86.909,21 315.000 126.606,28 - 247,64 83.930 43.546,011.282.930665.631,81

39. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  Beneficiary daily rate analysis

40. PROJECT LIFE12 ENV/IT/00036  ACTION E.1: Project management  Beneficiary daily rate analysis

41. PROJECT LIFE12 ENV/IT/00036 Project meetings  1st Kick off meeting in DISPAA in Italy in January 2014  Monitoring meeting in DISPAA in Italy in June 2014  2nd Coordination meeting in CEBASCSIC in Murcia (Spain) on July 7th 2014  3rd Coordination meeting in ASTRA in Italy on January 2015  4th Coordination meeting in DISPAA in Italy on June 2015 Next Project meeting  5th Final Coordination meeting in Soldano in Italy on December 2015