1. Morphological and genetic diversity of the larch populations on the Kuril Island I.Yu. Adrianova Group of Plant Molecular Genetics Institute of Biology and Soil Sciences, FEB RAS

2. To study the influence of island isolation on the morphological and genetic diversity of the populations of Larix kamtchatica (Rupr.) Carr. from Iturup Island The purpose of this research: Slide 2

3. The schemes of location of the larch populations studied - place of the sample collecting Slide 3

4. the length of the seed wing The morphological traits of cones and seeds used in this work the length and width of the cone the ratio of length to width of the cone the deflection angle of scales in the cone The quantitative trait: The qualitative trait: the shape of the scales the character of scale edge the degree of deflection of the scale edge the number of scales in the cone the length and width of the seed Slide 4

5. Level of variabilityCV, %Level of variabilityCV,% Very low< 7Higher21-30 Low8-12High31-40 Medium13-20Very high>40 Levels of variability of morphological traits of wood species worked out by S.A. Mamaev CV – coefficient of variation Individual variability of all morphological traits ranged from 8 to 21 %. AMOVA analysis based on all morphological traits doesn’t distinguish these populations (p = 0.204). Slide 5

6. PCA ordination of the larch individuals using all morphological traits Slide 6 Kas- Kasatka Khv - Khvoinyi

7. The dendrogram of relationship between trees from two larch populations of Iturup Island based on all morphological traits of cones and seeds Kas- Kasatka Khv– Khvoinyi Slide 7

8. Slide 8 SampleAP 95,%P 99,%HeIh Kasatka 1.3938.9 0.1339 0.12700.19 Khvoinyi 1.5543.350.50.14940.15070.23 The genetic estimations of our populations A - Observed number of alleles; P 95 - % polymorphic loci (95% criterion) ; P 99 - % polymorphic loci (99% criterion); He – average expected heterozygosity; I -Shannon's Information index [Lewontin (1972)]; h = Nei's (1973) gene diversity Larix sibirica Ldb. - A = 1.54; P 95 = 45%; He = 0.1698 Larix gmelinii (Rupr.) Rupr. - A= 1.32; P 95 = 32%; He = 0.1373 X-sq : 327.6221 df : 638 Overall: p = 1.0000 Exact tests for population differentiation (Raymond and Rousset, 1995)

9. Slide 9 UPGMA unrooting tree of the individuals from two larch populations based on Nei’s (1978) genetic distances Kas- Kasatka Khv– Khvoinyi Nm = 5.1

10. Conclusions: The island isolation have not caused a decrease of morphological and genetic diversity of the larch populations from Iturup Island Slide 10

11. Thank you for your attention !