1. Selected and Differential Media

2. Define Selective Media Differential Media Enriched Media

3. Blood Agar Plate (BAP) Differential Enriched Use  Growth  Hemolysis Alpha Beta Gamma

4. C-CNA Selective Differential Results  Gram (+) Colistin Naldixic acid  Blood Hemolysis

5. MSA Selective Differential Results  Halotolerant (7.5% NaCl)  CH2O: Mannitol  pH phenol red Fermentation  Acid  Yellow

6. MAC Selective Differential Results  Gram (-) CV Bile Salts  Lactose  pH indicator Neutral red indicator  Ferment = pink/red  No acid = colorless

7. EMB Selective Differential Results  Gram (-) Eosin dye MB dye  Lactose Some acid = pink Large acid = green No acid = colorless

8. Set Up One plate for each group Label quadrants as lab book indicates for each plate Cultures on cart Anaerobic Gas Pack  BAP  C-CNA All others aerobic 37°C

9. BAP results Sheep blood enriched Growth most all microbes Fastidious Differential  GP Hemolysins  Hemolysis types Gamma Alpha Beta

10. Hemolysis No hemolysis

11. C-CNA results Selective  Growth GP Colistin Nalidixic acid Differential  Hemolysis

12. MSA results Selective 7.5% NaCl Staphylococcus sp Differential Mannitol fermentation phenol red  yellow

13. MAC results Selective: GN  CV  Bile Salts Differential  Lactose fermentation  NF = colorless  Coliforms Neutral red  pink

14. EMB results Selective: GN  Dyes Eosin MB Differential  Lactose fermentation  NF = colorless  Acid production Small = pink Large = metallic green