1. Aflatoxin Contamination: Occurrence and Management Thomas Isakeit Cooperative Extension, The Texas A&M University System

2. AFLATOXINS: WHAT ARE THEY? - TOXIC CHEMICALS PRODUCED BY FUNGI (Aspergillus flavus and A. parasiticus) GROWING IN CROPS - CAUSE DISEASE IN ANIMALS AND HUMANS (MYCOTOXICOSIS) - MAY BE PRODUCED IN THE FIELD OR AFTER HARVEST - AFFECT MANY TYPES OF CROPS

3. TYPES OF AFLATOXINS STRUCTURE OF B 1, THE MOST POTENT AFLATOXIN, SPECIFICALLY REGULATED IN SOME COUNTRIES ALSO: B 2, G 1 AND G 2 M 1 AND M 2 IN MILK: SPECIFICALLY REGULATED

4. AFLATOXIN TOXICITY - HUMANS HEPATIC AND GASTROINTESTINAL INJURY IMMUNOSUPPRESSIVE TERATOGENIC ONCOGENIC SEVERE LIVER INJURY, SOMETIMES FATAL (2-6 MG/DAY FOR A MONTH)

5. AFLATOXIN TOXICITY - ANIMALS LUNG LESIONS IN A PIG POISONED BY AFLATOXIN VARIOUS SYMPTOMS: FROM REDUCED GAIN TO DEATH VARYING SENSITIVITY AMONG SPECIES

6. AFLATOXIN POISONING FROM CORN – RECENT CASES TEXAS, 1998: 25 DOGS DIED 100-200 PPB, FULL DIET FOR 90 DAYS KENYA, 2004: 125 PEOPLE DIED, 192 POISONED DAMP STORAGE OF CORN, UP TO 8,000 PPB

7. LOSS FROM AFLATOXIN IN TEXAS CORN IN 1998 YIELD LOSS FROM DROUGHT PRICE DROP ($2.20/BU vs. $2.80) $407 MILLION VALUE W/O TOXIN 18 – 31% CROP CONTAMINATION TOXIN VALUE: $1.30 - $1.40 / BU $17 - $40 MILLION LOSS

8. HISTORY IN TEXAS AFLATOXIN? LOSING TURKEYS: “I CUT THEM OPEN AFTER THEY DIE AND FIND A BIG LIGHT COLLORD LIVER; THE GIZZARD IS ENLARGED, ALSO THE GALL IS TWICE AS LARGE AS SHOULD BE.” - LETTER TO FARM AND RANCH, MAY 19, 1917 FROM VAN ZANDT COUNTY, TX

9. HISTORY IN TEXAS J.J. Taubenhaus documents A. flavus as a pathogen

10. A. FLAVUS IS A WEAK PATHOGEN THAT DOES NOT ALWAYS PRODUCE SYMPTOMS ASPERGILLUS EAR ROT PHOTO: G. ODVODY

11. …CONSEQUENTLY, AFLATOXIN DETECTION REQUIRES A CHEMICAL ANALYSIS EXTRACTION PURIFICATIONANALYSIS DETECTION IS DIFFICULT!

12. SAMPLING IS MORE DIFFICULT! CONTAMINATION IS NOT UNIFORM: - WITHIN A REGION - WITHIN A FIELD - ON A PLANT - WITHIN AN EAR - WITHIN A SEED

13. AFLATOXIN DISTRIBUTION IN TEXAS 2002 CORN ACREAGE 2003 CONTAMINATION

14. COMPILED BY: OFFICE OF THE TEXAS STATE CHEMIST

16. AFLATOXIN IN AN EAR OF CORN PHOTO: G. SCHUSTER CONCENTRATIONS ARE DIFFERENT FOR EACH KERNEL

17. WHERE AFLATOXIN OCCURS IN A CORN SEED (SHOWN IN RED) PHOTO: N. KELLER AS SHOWN BY THE RED COLOR MUTANT, AFLATOXIN IS FOUND IN THE ALEURONE

18. A SMALL PROPORTION OF CORN IS HIGHLY CONTAMINATED: 1 KERNEL WITH 400,000 PPB WILL MAKE A 10-LB SAMPLE MEASURE 26 PPB

19. WHERE DOES THE FUNGUS COME FROM? Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. airborne infective spores survival in soil MODIFIED FROM: WICKLOW, 1983, SO. COOP. SER. BUL. 279 BROWN & BROTZMAN, 1979, U. OF MO.

20. SCLEROTIA OF A. FLAVUS DETAIL: RED BAR IS APPROX. 1 MM

21. HOW THE FUNGUS ENTERS THE EAR Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. SPORES FALLING INTO WOUNDS CAUSED BY INSECTS (E.G. CORN EARWORM AND SOUTHWESTERN CORN BORER), OR CARRIED ON THEM CAN GROW DOWN SILKS (NO WOUNDING NECESSARY), SHORTLY AFTER POLLINATION

22. HOW THE FUNGUS ENTERS THE SEED Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. A WOUND IS USUALLY NEEDED

23. SILK CUT STRESS-RELATED LOSS OF KERNEL INTEGRITY PHOTO: G. ODVODY

24. PREDISPOSING FACTORS FOR CONTAMINATION Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. DROUGHT HIGH TEMPERATURES AT FLOWERING INSECT INJURY STRESS ON PLANT + INCREASE IN PATHOGEN REPRODUCTION

25. FACTORS AFFECTING AFLATOXIN PRODUCTION ACTIVITY DOES NOT START UNTIL KERNEL MOISTURE <32% BEST RANGE IS 16-20% MOISTURE, 18% IS OPTIMAL OPTIMAL 77-86 o F, BUT FUNGUS GROWS WELL AT 97 o F

26. AFLATOXIN MANAGEMENT IN THE FIELD (PRE-HARVEST) - host resistance - insect control - cultural practices - biological treatment USE OF SEVERAL APPROACHES

27. HOST RESISTANCE - TO INSECT, CONVENTIONAL & TRANSGENIC (Bt): NOT ENOUGH - TO FUNGUS: SOURCES IN LINES, NOT EASY TO INTRODUCE INTO HYBRIDS - IMPROVED HOST TOLERANCE TO STRESS

28. HUSK COVER IS A TRAIT ASSOCIATED WITH LESS AFLATOXIN PHOTO: G. ODVODY

29. HUSK COVER AND AFLATOXIN Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. A TREND TOWARDS MORE AFLATOXIN WITH AN OPEN HUSK BETRAN & ISAKEIT, 2004. AGRON. J. 96:565

30. AFLATOXIN AND HYBRID MATURITY Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. BETRAN & ISAKEIT, 2004. AGRON. J. 96:565 MORE AFLATOXIN WITH EARLY-MATURING VARIETIES

31. AFLATOXIN AND MATURITY Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. A TREND TOWARDS LESS AFLATOXIN IN HYBRIDS WITH A LONGER MATURITY BETRAN & ISAKEIT, 2004. AGRON. J. 96:565

32. CORN BREEDING AND AFLATOXIN EVALUATION FOR RESISTANCE REQUIRES MORE TIME AND LABOR THAN OTHER TRAITS

33. PESTICIDE TREATMENTS INSECTICIDES TARGETED TO: - REDUCE PLANT STRESS (E.G. PRESCRIBE FOR ROOT INSECTS) - REDUCE EAR INJURY FUNGICIDES ARE NOT EFFECTIVE OR PRACTICAL

34. CULTURAL PRACTICES - PLANTING DATE - PLANT POPULATIONS - WEED & PEST CONTROL - IRRIGATION - TILLAGE TO BREAK HARD PAN - ADEQUATE FERTILITY

35. CULTURAL PRACTICES WHATEVER YOU DO TO GET THE BEST CROP POSSIBLE DOES NOT CONFLICT WITH MYCOTOXIN MANAGEMENT

36. BIOLOGICAL CONTROL - AF36 LABELED FOR COTTON IN TX AND AZ - AFLA-GUARD: GA PEANUTS - APPLIED EARLY SEASON TO COVER FIELD - AREA-WIDE TREATMENT - CARRY OVER (PLACE/TIME)

37. HARVEST PRACTICES - SEGREGATE HARVEST OF “GOOD” AND “BAD” FIELDS -CHANGE COMBINE SETTINGS TO REMOVE DAMAGED SEED - EARLY HARVEST, FOLLOWED BY ARTIFICIAL DRYING - CLEANING SEED AFTER HARVEST (E.G. DENSITY SEPARATOR)

38. MYCOTOXINS IN INTACT AND DAMAGED/DISCOLORED KERNELS “GOOD”: 35 PPB AFLATOXIN 1 PPM FUMONISIN DAMAGED OR DISCOLORED: 270 PPB AFLATOXIN 9.3 PPM FUMONISIN REPRESENTATIVE DAMAGED KERNELS

39. AFLATOXIN CONCENTRATION CAN INCREASE THE LONGER CORN IS LEFT IN THE FIELD DATA OF G. ODVODY

40. AFLATOXIN: POST-HARVEST MANAGEMENT Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. PROPER STORAGE CONDITIONS TO MINIMIZE INCREASE BLENDING WITH “CLEAN” CORN – SUBJECT TO FEDERAL AND STATE REGULATIONS – CHECK FIRST! CHEMICAL NEUTRALIZATION IS SUBJECT TO FEDERAL AND STATE LAWS – CHECK FIRST!

41. STORAGE CONDITIONS Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. OBJECTIVE IS TO KEEP THE FUNGUS WITHIN THE GRAIN DORMANT STORE CORN AT <15% MOISTURE AND KEEP OUT MOISTURE PROPER OPERATION OF VENTILATION TO PREVENT MOISTURE CONDENSATION CAUSED BY UNEVEN TEMPERATURES IN BIN

42. MOISTURE MIGRATION IN A BIN DIAGRAM: ROSS ET AL., 1974 U. KY. AEN-20 TEMPERATURE DIFFERENCES WITHIN THE BIN LEAD TO AIR MOVEMENT, WITH MOISTURE CONDENSATION AT THE TOP OF THE BIN. PROPER VENTILATION WILL PREVENT THIS.

43. CHEMICAL TREATMENTS Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. Figure 3. Aflatoxin content of early, intemediate and full season hybrids Figure 4. Relationship between aflatoxin content and maturity. Figure 5. Husk cover of early, intemediate and full season hybrids Figure 6. Relationship between husk cover and aflatoxin content.. AMMONIATION: SUBJECT TO STATE AND FEDERAL REGULATIONS OZONATION: NOT COMMERCIALLY AVAILABLE ABSORBENT CLAYS ADDED TO FEED (E.G. NOVASIL): NOT YET APPROVED BBY REGULATORY AGENCIES

44. ENTEROSORBENT CLAY ADDITIVE AFLATOXIN PRESENT IN FEED NOVASIL ADDED TO CONTAMINATED FEED PHOTOS: OFFICE OF THE TEXAS STATE CHEMIST

45. OZONATION OF CONTAMINATED CORN: EFFECT ON GROWTH PHOTO: MCKENZIE ET AL. POULTRY SCI., 1998 CONTROLO3 CKAFLATOXIN AF+O3 APPEARANCE OF LIVERS