1. Soy bean using iplant resources

2. How we started iPlant workshop –CSHL

3. Introduction 14 different samples – Seeds 5, 6 and 7 stages well watered and drought stress – Different parts of Roots And 2 time points 72 and 96 hours as well well watered and drought stress

4. Analysis-Quality checks We performed quality checks using FASTX toolkit – Locally as well as in DE

5. Analysis Tuxedo suite of tools Bowtie for the alignment of the filtered data Cufflinks suite for the analysis We are checking the needs of the project so that we can add more functionalities into our pipelines

6. Quality Analysis SWS-R5 SWS-R6SWS-R7SWW-R5 SWW-R6 SWW-R7 Seeds 96-LRS-R2 Lateral Root 96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1

7. GC content SWS-R5 SWS-R6SWS-R7SWW-R5 SWW-R6 SWW-R7 Seeds 96-LRS-R2 Lateral Root 96-LRS-R1 96-LRC-R2 96-LRC-R1 72-LRS-R2 72-LRS-R1 72-LRC-R2 72-LRC-R1