1. 1 isr Institute for Sustainable Resources CRICOS No. 00213J Automated Greenhouse Gas Measurement System

2. isr Institute for Sustainable Resources CRICOS No. 00213J Basic Principle List of Components: A- SRI GC B- ECD Injector 8 Port C- FID Injector 8 port D- Flow meter E- Sample/ Calibration Standard Valve F- Sample Valve on Manifold G- ECD Detector H- FID Detector I - Needle Valve 2

3. isr Institute for Sustainable Resources CRICOS No. 00213J Set 1 Set 3 Set 2 3 hour Cycle Chamber 1-4 Chamber 5-8 Chamber 9-12 N 2 O increase 3 minutes per sample 4 treatments applying 3 replicate chambers measured at 4 X 15 min intervals 3 ml volume of sample Replicates Calibration Standard Cycle Schematic Start/ Restart 3

4. isr Institute for Sustainable Resources CRICOS No. 00213J Sampling Unit Sensor Inputs Sample Ports PLC Unit CO 2 Analyser 3 ml Sample Loops Suction Pump Flow Meter Chamber Set Switches 4

5. isr Institute for Sustainable Resources CRICOS No. 00213J General Overview PLC Embedded Computer Actuators Sensors Embedded computer: Wireless connection to outside (forwarding data to server) Graphical user interface for operating system Data logging tool Automated data analysis Trouble shooting by informing user per email 5

6. 6 isr Institute for Sustainable Resources CRICOS No. 00213J PLC (Programmable Logic Controller) as Sensor Board Gas chromatograph Soil moisture sensor CO 2 analyser Rain gauge Pt100 temperature probes Flow meters Pressure transmitters... PLC

7. isr Institute for Sustainable Resources CRICOS No. 00213J PLC PLC as Operator Suction pump Sample injection valve Sample valves Relays Pneumatic cylinders Electronic chambers... 7

8. isr Institute for Sustainable Resources CRICOS No. 00213J High temporal resolution: - 32 samples chamber -1 day -1 : 8 flux rates day -1 Sampled at constant and accurate intervals Automated data acquisition reduces human errors From monotonous work to research! Systems operate autonomous and can be controlled and checked via internet Research is only as good as the data 8

9. 9 isr Institute for Sustainable Resources CRICOS No. 00213J User Interface Dynamic element to show user which chamber is currently sampled Sequence count down

10. isr Institute for Sustainable Resources CRICOS No. 00213J 10 Manual Control

11. isr Institute for Sustainable Resources CRICOS No. 00213J 11 Data Logging

12. isr Institute for Sustainable Resources CRICOS No. 00213J 12 Data Logging Data logging in the back ground: Apart from the optional items it automatically logs alarms and system status

13. isr Institute for Sustainable Resources CRICOS No. 00213J 13 File Name Filename includes: Sensor item name Date of log file number in case sequencly logs are configured Log entry as following order: Date;Timestamp;ValueTemperature1; ValueTemperature2;ValueTemperature3

14. isr Institute for Sustainable Resources CRICOS No. 00213J 14 Chart Tool