1. FORENSIC CHEMISTRY AS SCIENTIFIC AND EDUCATIONAL SPECIALISATION AT THE JAGIELLONIAN UNIVERSITY Paweł Kościelniak JAGIELLONIAN UNIVERSITY, Faculty of Chemistry INSTITUTE OF FORENSIC RESEARCH Cracow, Poland

2. Jagiellonian University (1364)

3. Faculty of Chemistry 1950-52 1929 Institute of Forensic Research

4. DEPARTMENT OF ANALYTICAL CHEMISTRY (25) GROUP OF TOXICOLOGICAL AND PHARMACEUTICAL ANALYSIS (5) GROUP OF ANALYTICAL FLOW TECHNIQUES (11) GROUP OF CHEMOMETRICS (3) LABORATORY FOR FORENSIC CHEMISTRY

5. LABORATORY FOR FORENSIC CHEMISTRY - system for capillary electrophoresis (PrinCe 550 CSD, Prince) - HPLC chromatograph (LaChrom, Merck) - GC chromatograph (3900, Varian) - atomic fluorescence spectrometer with hydride generation (AFS 230, Beijing Haiguang Instrument Co.) - system for microwave digestion (MARS 5X, CEM), - audiovisual equipment (video-projectors, digital photo-cameras, video-camera, video-recorders) founded in 2000

6. Sem. Subject No. of hours lecturesclassesseminarstotal VI Electronics and automatics30 Optional60 Total90 VII Electronics and automatics60 Advanced analytical chemistry45 Chemometrics and biometrics301545 Analysis in forensic chemistry15 Radiometric analysis30 Toxicology30 Flow analysis15 Optional60 Total2256015300

7. Sem. Subject No. of hours lecturesclassesseminarstotal VIII Radiometric analysis 45 Forensic toxicology 601575 Criminalistics15601590 Low aspects of the forensic expert work 15 Student’s report 30 Optional60 Total100 315 IX i X Laboratory for master thesis 450 Seminar for master thesis 60 Clinical toxicology15 GLP15 Optional60 Total9045060600

8. No. Subject Place 1Toxicological analysis of drugsIFR 2Ethanol- selected toxicological and analytical aspectsIFR 3Application of GC/MS method to analysis of drugs in biological materialsIFR 4Determination of inorganic toxicants in biological materialIFR 5Chemical examinations of car paintsIFR 6Chemical examinations of fibersIFR 7Chemical examinations of gun-shot residualsIFR 8Chemical examination of inks by TLCIFR 9Chemical analysis of inks by CEJU 10Determination of Se and As in biological materials by AFS-HGJU 11Comparative examination of psychotropic drugs by HPLC and CEJU 12Profiling of drugs – physicochemical and chemometric examinationJU LABORATORY CLASSES

9. Subject Analytical method FCh JUIFR Determination of selected elements at normal levels in biological materials ICP/OES, AAS AAS Development of novel techniques for preparation of biological samples HPLC, CE Development of analytical procedures for determination of psychotropic drugs HPLC, CE Development of analytical procedures for determination of selenium and arsenic in biological materials AFS Application of Py-GCMS to analysis of criminalistic traces Py-GCMS Identification and discrimination of paint traces chemometric methods IR Examination of questioned documents on the basis of identification of chemical composition of inks CE, LIBS RESERCH SUBJECTS

10. PUBLICATIONS M. Zadrożna, M. Gawlik, B. Nowak, A. Marcinek, H. Mrowiec, S. Walas, R. Wietecha-Posłuszny, P. Zagrodzki, „Antioxidants Activities and Concentration of Selenium, Zinc and Copper in Preterm and IUGR Human Placentas”, J. Trace Elem. Med. Biol., 23, 144-148 (2009). M. Szafarska, M. Woźniakiewicz, M. Pilch, J. Zięba-Palus, P. Kościelniak, “Computer Analysis of ATR-FTIR Spectra of Paint Samples for Forensic Purposes”, J. Molec. Struct., 924–926, 504–513 (2009). B. Trzcińska, J. Zięba-Palus, P. Kościelniak, "Application of Microspectrometry in Visible Range to Differentiation of Car Solid Paints for Forensic Purposes", J. Molec. Struct., 924–926, 393–399 (2009). R. Wietecha-Posłuszny, J. Dobrowolska, P. Kościelniak, P. Zagrodzki, „Determination of Selenium as a Biomarker of Thyroid Cancer by HG-AFS Method”, Acta Chim. Slov., 56, 441-446 (2009). J. M. Milczarek, M. Dziadosz, J. Zięba-Palus, “Way to Distinguish Car Paint Traces Based on Epoxy Primer Layers Analysis by Pyrolysis - Gas Chromatography - Mass Spectrometry (Py-GC/MS)”, Chem. Anal., 54, 173-185 (2009. K. Madej, „Microwave-assisted and Cloud-point Extraction in Dtermination of Drugs and Other Bioactive Compounds”, Trends in Analytical Chemistry, 28, 436-446 (2009). K. Madej, A. Biedroń, A. Garbacik, „Study of Separation and Extraction Conditions for Five Neuroleptic Drugs by an LLE-HPLC-DAD Method in Human Plasma”, Journal of Liquid Chromatography & Related Technologies, 32, 3025-3037 (2009). E. Florek, E. Ignatowicz, W. Piekoszewski, “Effect of Pregnancy and Tobacco Smoke on the Antioxidant Activity of Rutin in an Animal Model”, Pharmacological Reports, 61, 935-940 (2009). M. Woźniakiewicz, J. Kuczara, P. Kościelniak, „Determination of Fluoxetine in Blood Samples by High-performance Liquid Chromatography Using a Derivatization Reagent”, Chem. Anal. (Warsaw), 54, 667-677 (2009). R. Wietecha-Posłuszny, T. Lech, P. Kościelniak, “Application of Three Spectrometric Methods to Total Selenium Determination in Post- mortem Material in a Case of Acute Selenium Compound Poisoning”, J. Forensic Sci., in print. M. Szafarska, R. Wietecha-Posłuszny, M. Woźniakiewicz, C. Hughes, P. Kościelniak, „Influence of Storage Conditions on Ageing of Colour Dye-Based Inkjet Printing Inks”, Problems of Forensic Sciences, in print

11. Ph.D. STUDY StudentSubjectDefense Renata Wietecha-Posłuszny Development of the analytical procedure for the selenium determination in human tissues by the AFS-HG technique 2004 Rafał BorusiewiczExamination of arson traces2004 Joanna ManiaExamination of inks by capillary electrophoresis2005 Michał WoźniakiewiczAnalysis of psychotropic drugs by HPLC and CE2008 Justyna DobrowolskaElemental analysis of biological materials2008 Jakub MielczarekClassification and discrimination of car paint traces2008 Justyna Dobrowolska Elemental analysis of selected human and animal tissues with toxicological and clinical respects 2009 Małgorzata Szafarska Development of the method for discrimination of printer inks by capillary electrophoresis for criminalistic purposes 2010 Jagoda MajcherczykInfluence of tobacco smoke on pharmacokinetics of citalopram2011 Aneta GarbacikNovel methods for preparation of biological samples to toxicological analysis2011 Danuta Dudek-AdamskaTrace elements in biological materials2011 Agnieszka Biedroń Analytical and toxicological examination of selected drugs in biological materials 2012 Paweł KnihnickiDetermination of psychotropic drugs in saliva by capillary electroforesis2012 Tomasz Lachowicz Py-GCMS analysis as a tool in forensic examination of criminalistic traces 2012 Agnieszka SolarzAnalysis of printer inks by capillary electrophoresis for criminalistic purposes2013

12. PROJECTS TEMPUS program, project „Transformation of University Chemistry Studies at the Jagiellonian University” (TRUCS); 1997-2000 TEMPUS program, project „National Training Program for Forensic Science Experts” (NATFOSE); 1998-2001 LEONARDO DA VINCI program, project „Vocational Training System in Forensic Sciences - Exchange of Educational Experiences” (VITSFOS-E); 2001-2003 NATO program, project “Sensors for Chemical Weapons Detection Based on Molecularly Imprinted Polymers”; 2004-2005 TEMPUS program, project „Education System in Forensic Sciences for the Republic of Macedonia”, (EDUFORMAK); 2007-2010 Government Grant, project „Application of the Capillary Electrophoresis as a Tool in Criminalistic Examination of Ink Printed Documents”; 2007-2010 Operational Programme 'Innovative Economy', project „Mikro and Nano-systems in Chemistry and Clinical Diagnostics” (MNS-DIAG); 2009-2013 Government Grant, project „The Use of Modern Analytical Instrumentation for Purpose of the Fight Against Crime”; 2010-2013

13. Laser-induced breakdown spectrometer ( (LIBS) Laser-induced breakdown spectroscopy (LIBS) is a type of atomic emission spectroscopy, which uses a highly energetic laser pulse as the excitation source. The laser is focused to form a plasma, which atomizes and excites samples. Price: ~ 45 000 EUR PLASMA EMMISION PULSED LASER (1064 nm) PLASMA PLUME SAMPLE