1. AP Biology 8/25/2015 Chapter 18. Viral Genetics

2. AP Biology 8/25/2015 Viral diseases Measles Polio Hepatitis Chicken pox

3. AP Biology 8/25/2015 Influenza: 1918 epidemic 30-40 million deaths world-wide RNA virus

4. AP Biology 8/25/2015 Smallpox  Eradicated in 1976  vaccinations ceased in 1980  at risk population?

5. AP Biology

6. 8/25/2015 Emerging viruses  Viruses that “jump” host  switch species  Ebola, SARS, bird flu, hantavirus SARS Ebola hantavirus

7. AP Biology Scene from the 2003 SARS Scare

8. AP Biology SARS (severe acute respiratory syndrome), a recently emerging viral disease (a) Young ballet students in Hong Kong wear face masks to protect themselves from the virus causing SARS. (b) The SARS-causing agent is a coronavirus like this one (colorized TEM), so named for the “corona” of glycoprotein spikes protruding from the envelope.

9. AP Biology 8/25/2015 A sense of size  Comparing size  eukaryotic cell  bacterium  virus

10. AP Biology T4 bacteriophage infecting an E. coli cell 0.5  m

11. AP Biology 8/25/2015 What is a virus? Is it alive?  DNA or RNA enclosed in a protein coat  Viruses are not cells  Extremely tiny  electron microscope size  smaller than ribosomes  ~20–50 nm 1 st discovered in plants (1800s)  tobacco mosaic virus  couldn’t filter out  couldn’t reproduce on media like bacteria

12. AP Biology Infection by tobacco mosaic virus (TMV)

13. AP Biology 8/25/2015 Variation in viruses plant viruspink eye  Parasites  lack enzymes for metabolism  lack ribosomes for protein synthesis  need host “machinery”

14. AP Biology 8/25/2015 Variation in viruses bacteriophageinfluenza  A package of genes in transit from one host cell to another “A piece of bad news wrapped in protein” – Peter Medawar

15. AP Biology Viral structure 18  250 mm 70–90 nm (diameter) 80–200 nm (diameter) 80  225 nm 20 nm50 nm (a) Tobacco mosaic virus(b) Adenoviruses(c) Influenza viruses (d) Bacteriophage T4 RNA Capsomere of capsid DNA Capsomere Glycoprotein Membranous envelope Capsid DNA Head Tail fiber Tail sheath

16. AP Biology 8/25/2015 Viral genomes  Viral nucleic acids  DNA  double-stranded  single-stranded  RNA  double-stranded  single-stranded  Linear or circular  smallest viruses have only 4 genes, while largest have several hundred

17. AP Biology 8/25/2015 Viral protein coat  Capsid  crystal-like protein shell  1-2 types of proteins  many copies of same protein

18. AP Biology 8/25/2015 Viral envelope  Lipid bilayer membranes cloaking viral capsid  envelopes are derived from host cell membrane  glycoproteins on surface HIV

19. AP Biology Virus Invasion

20. AP Biology 8/25/2015  Entry  virus DNA/RNA enters host cell  Assimilation  viral DNA/RNA takes over host  reprograms host cell to copy viral nucleic acid & build viral proteins  Self assembly  nucleic acid molecules & capsomeres then self- assemble into viral particles  exit cell Generalized viral lifecycle

21. AP Biology 8/25/2015 Symptoms of viral infection  Link between infection & symptoms varies  kills cells by lysis  cause infected cell to produce toxins  fever, aches, bleeding…  viral components may be toxic  envelope proteins  Damage?  depends…  lung epithelium after the flu is repaired  nerve cell damage from polio is permanent

22. AP Biology 8/25/2015 Viral hosts  Host range  most types of virus can infect & parasitize only a limited range of host cells  identify host cells via “lock & key” fit  between proteins on viral coat & receptors on host cell surface  broad host range  rabies = can infect all mammals  narrow host range  human cold virus = only cells lining upper respiratory tract of humans  HIV = binds only to specific white blood cells

23. AP Biology 8/25/2015 Bacteriophages  Viruses that infect bacteria  ex. phages that infect E. coli  lambda phage  20-sided capsid head encloses DNA  protein tail attaches phage to host & injects phage DNA inside

24. AP Biology 8/25/2015 Bacteriophage lifecycles  Lytic  reproduce virus in bacteria  release virus by rupturing bacterial host  Lysogenic  integrate viral DNA into bacterial DNA  reproduce with bacteria

25. AP Biology The lytic cycle of phage T4, a virulent phage Attachment. The T4 phage uses its tail fibers to bind to specific receptor sites on the outer surface of an E. coli cell. Entry of phage DNA and degradation of host DNA. The sheath of the tail contracts, injecting the phage DNA into the cell and leaving an empty capsid outside. The cell’s DNA is hydrolyzed. Synthesis of viral genomes and proteins. The phage DNA directs production of phage proteins and copies of the phage genome by host enzymes, using components within the cell. Assembly. Three separate sets of proteins self-assemble to form phage heads, tails, and tail fibers. The phage genome is packaged inside the capsid as the head forms. Release. The phage directs production of an enzyme that damages the bacterial cell wall, allowing fluid to enter. The cell swells and finally bursts, releasing 100 to 200 phage particles. 1 2 4 3 5 Phage assembly Head Tails Tail fibers

26. AP Biology 8/25/2015 Lysogenic lifecycle of phages

27. AP Biology Lysogenic Virus: Herpes 8/25/2015

28. AP Biology

29. Herpetic Whitlow 8/25/2015  http://www.nlm.nih.gov/medlineplus/enc y/imagepages/1373.htm http://www.nlm.nih.gov/medlineplus/enc y/imagepages/1373.htm

30. AP Biology Herpes Simplex II 8/25/2015

31. AP Biology 8/25/2015 Defense against viruses  Bacteria have defenses against phages  bacterial mutants with receptors that are no longer recognized by a phage  natural selection favors these mutants  bacteria produce restriction enzymes  recognize & cut up foreign DNA

32. AP Biology 8/25/2015 RNA viruses  Retroviruses  have to copy viral RNA into host DNA  enzyme = reverse transcriptase  RNA  DNA  mRNA  host’s RNA polymerase now transcribes viral DNA into viral mRNA  mRNA codes for viral components  host’s ribosomes produce new viral proteins proteinRNADNA transcriptiontranslation replication

33. AP Biology 8/25/2015 Retroviruses  HIV  Human ImmunoDeficiency Virus  causes AIDS  Acquired ImmunoDeficiency Syndrome  opportunistic diseases  envelope with glycoproteins for binding to specific WBC  capsid containing 2 RNA strands & 2 copies of reverse transcriptase

34. AP Biology Retrovirus 8/25/2015

35. AP Biology 8/25/2015 HIV infection  HIV enters host cell  macrophage & CD4 WBCs  cell-surface receptor  reverse transcriptase synthesizes double stranded DNA from viral RNA  high mutation rate  Transcription produces more copies of viral RNA  translated into viral proteins  proteins & vRNA self-assemble into virus particles  released from cell by “budding” or by lysis

36. AP Biology Reverse Transcriptase 8/25/2015

37. AP Biology 8/25/2015 HIV treatments  inhibit vRNA replication  AZT  thymine mimic  protease inhibitors  stops cleavage of polyprotein into capsid & enzyme proteins

38. AP Biology 8/25/2015 Potential HIV treatments  Block receptors  chemokines  bind to & block cell-surface receptors  11% of Caucasians have mutant receptor allele  Block vRNA replication

39. AP Biology 8/25/2015 Cancer viruses  Viruses appear to cause certain human cancers  hepatitis B virus  linked to liver cancer  Epstein-Barr virus = infectious mono  linked to lymphoma  papilloma viruses  linked with cervical cancers  HTLV-1 retrovirus  linked to adult leukemia

40. AP Biology 8/25/2015 Cancer viruses  Transform cells into cancer cells after integration of viral DNA into host DNA  carry oncogenes that trigger cancerous characteristics in cells  version of human gene that normally controls cell cycle or cell growth  Most tumor viruses probably cause cancer only in combination with other mutagenic events

41. AP Biology 8/25/2015 Prions  Misfolded proteins  infectious  make plaques (clumps) & holes in brain as neurons die Creutzfeldt-Jakob disease “mad cow” disease

42. AP Biology 8/25/2015 Protein as information molecule?!  Prions challenge Central Dogma  transmit information to other proteins Stanley Prusiner UC School of Medicine 1982 | 1997 proteinaceous infectious molecule PnPn PdPd

43. AP Biology Review Questions

44. AP Biology 1. Viruses are not classified as prokaryotes because: A. They contain membrane bound organelles B. They are multicellular C. They are unicellular D. They are acellular E. They lack genetic material

45. AP Biology 2. Which of the following can be found in all viruses: A. Ribosomes B. RNA C. DNA D. A Protein Coat E. Cell membrane

46. AP Biology  The virus HIV replicates using reverse transcriptase. Thus it can be inferred that the virus  Uses only DNA  Uses only RNA  Uses RNA as a template for DNA  Uses DNA as a template for RNA  Replicates continuously