1. Evaluation of antimicrobial agents and Neisseria species Lab # 7 Medgar Evers College Prof. Santos

2. Exercise 36 AIM Using the Kirby Bauer method to determine antibiotic effect. Know the difference between an antibiotic and antimicrobial agent zone of inhibition is the area where no growth of bacteria is evident! Next week, you will bring a clear ruler to measure the diameter of the zone of inhibition. The recommended medium is Mueller Hinton II agar. Disks containing the appropriate antibiotics are placed on the agar and the following week the zone of inhibition is measured. The greater the diameter, the more effective the antibiotic is or the more sensitive the bacterium is to that particular antibiotic.

5. Exercise 37 AIM You will test the effectiveness of various antimicrobial agents such as phenol, Lysol, Iodine, and formaldehyde. You soak half of a paper disk into the antiseptic and placed it on the agar and the following week you will determine the radius to evaluate its effectiveness. You will use the pour plate method! Inoculate the flask of agar when it has cooled down and then quickly pour it on the petri dish! WAIT for it to solidify before placing disk with antimicrobial agent!

6. Exercise 27 As far as exercise 27 is concerned know the 4 types of Neisseria species and what they cause. (gram – diplococcus) Organismpathogenicity N. gonorrhoeaeSTD Gonorrhea N. meningiditismeningitis N. siccaNormal flora of respiratory tract N. flavescensNormal flora of respiratory tract

7. 2 medically important species are Neisseria gonorrhoeae and Neisseria meningitidis They are very sensitive to environmental conditions outside the body. Both N. gonorrhoeae and N. meningitidis are easily destroyed in specimens or samples that are 1-Delayed in transit to the laboratory 2-Kept in temperature too far below or above 35 0 C 3-Heavily contaminated with normal flora 4-Not provided with an adequate supply of carbon dioxide.