1. Impression Cytology: Shipping Dry Eye Assessment and Management Penny Asbell, MD, MBA Brendan Barry, BA Seth Epstein,MD Yi Wei, PhD

2. IC Objective  Minimally invasive objective biomarker for inflammation of the ocular surface  Samples are stained with antibodies and analyzed by Flow Cytometry to identify effector cells  Evaluation of Treatment Efficacy

3. Effector Cells in DREAM

4. Materials for Shipping: Com-Pac INF-3011 INFECON 3000 Standard-grade packing tape Scotch Tape Scissors

5. IATA Certification Only IATA Certified personnel can ship. Every site should identify and train, at least one (1) individual by a recognized training course: – https://www.training.saftpak.com/ https://www.training.saftpak.com/ – Samples must be shipped via IATA Packing Instruction 602-certified materials as per IATA regulations and assembled as per manufacturer’s instructions.

6. Shipment Preparation An initial set of supplies will be provided. – INFECON-3000 Shipper Kits (x12) – Impression Cytology Materials Samples must be shipped within 2 weeks of collection – Sites should plan to ship twice a month – Ship with provided cold packs

7. 1. Fill Forms Copies of each sample’s Impression Cytology Registration Form

8. 2. Place Labels on Shipper

9. 3. Shipper Preparation Assemble shipper: – Insert the cold pack into the container – Insert the sample into the bubble bag (1) – Insert the bubble bag into the container (2) – Secure the lid (3) (1)(3)(2)

10. – Place the container into the shipper box – Insert the forms into the box (1) – Close and seal the box with packing tape (2,3) (1) (2) (3)

11. X 4. Use FedEx Label without “Dry Ice” Checked

12. 5. Final Box Attachments The following must be attached to the box: 1.Shipper Information 2.Consignee (individually receiving shipment; copy from FedEx label) 3.Responsible person (with phone number in case FedEx needs to contact shipper) Note: all three items can be on one Word document that is taped to the side Remember your Category B label!!

13. Impression Cytology : Summary Flow Cytometry provides an objective minimally invasive metric of ocular inflammation IC provides Biomarkers of Inflammation of the Ocular Surface May provide a method to better classify severity of DED Will add to our understanding of DED and the mechanism of action that leads to inflammation of the ocular surface

14. seth.epstein@mssm.edu (212)-241-5606