1. Extracted from presentation given by Robin Todd at 2007 Ent Soc. America meeting ICR, Inc. Robin Todd, ICR, Inc.

2. CleanZone A non-chemical method that works Elevated ozone levels – 100 ppm + Ozone Generator Confined spaces Used for mold elimination Bed bugs a new target Robin Todd, ICR, Inc.

3. Test CleanZone Systems LLC. Lisle, IL Conducted study in an empty home Showed efficacy Asked ICR for independent confirmatory test Robin Todd, ICR, Inc.

4. Safety EPA’s acceptable level: 0.08 ppm for 8 hours (0.07- 0.075 ppm proposed) CleanZone generates 100ppm + Test run in green house – safety Ozone levels monitored CleanZone staff attended test and ran generator Robin Todd, ICR, Inc.

5. Test details Test chamber built 8x18x7ft tall (1008 ft 2 ) Plastic sheeting over PVC piping Sand snakes hold down plastic Robin Todd, ICR, Inc.

6. Bed bug containment & Replication 10 containers with screened lids 10 containers covered with cloth 10 adult bed bugs per container Robin Todd, ICR, Inc.

7. Test Chamber Robin Todd, ICR, Inc.

8. Control Chamber 4x6x7 ft high chamber (168 ft 2 ) In a different building – no elevated ozone Robin Todd, ICR, Inc.

9. Other species tested German cockroaches Confused flour beetles Pharaoh ants American house dust mites Robin Todd, ICR, Inc.

10. Containers of insects Robin Todd, ICR, Inc.

11. Observations during exposure Ozone levels observed hourly One container of each species removed hourly - Knockdown checked = monitors Self-sealing port – avoid ozone loss Robin Todd, ICR, Inc.

12. Results Ozone in test chamber 118.3 ppm (mean) Generator run for 22.7 hours Peak (150.7 ppm) at 9 hours Still air – underestimated levels Ozone levels in control chamber: 0 – 0.1 ppm Robin Todd, ICR, Inc.

13. Ozone levels Robin Todd, ICR, Inc.

14. Results 100% knockdown in monitor containers for all species except ants Robin Todd, ICR, Inc.

15. Results Test Chamber - 100% mortality +24 hours after removal from chamber 4% mortality in control Robin Todd, ICR, Inc.

16. Hourly Knockdown Robin Todd, ICR, Inc.

17. Dead bed bugs after treatment no longer clinging to filter paper Robin Todd, ICR, Inc.

18. Other Species Treatment killed 100% of roaches and dust mites 86% (open) 98% (covered) of beetles 40% (open) 83% (covered) of ants –Ants in covered nest containers – because sheltered from ozone? Robin Todd, ICR, Inc.

19. Acknowledgement John Sharpe, formerly with ICR, conducted this test. He did a great job. Robin Todd, ICR, Inc.