1. The world leader in serving science Rhombix Solutions for Automated Crystallization 2007 RAMC

2. 2 Rhombix Product Line – supporting the crystallization workflow Semi-automated imaging Automated imaging and storage MaX Workcell Rhombix Software Suite

3. 3 Instrument-Centric to Data-Centric Automation Result: Isolated “islands” of automation and data stores -- No data connection Imaging and Storage Experiment design and analysis Liquid handler – Dispense screens into wells Nanodispenser Drop-making Xtallization Database

4. 4 New Product MaX Workcell Plate preparation New

5. 5 MaX Workcell  Features & Benefits All-inclusive product – pre-configured for quick installation Seamless Workflow Tube-to-plate transfer Plate-to-plate transfer Custom screen building (gradients, additives, etc) Nano Drop placement Sealing Multiple experiment design and submission Full set of SW tools incorporating experimental data and automation control Small footprint

6. 6 Imaging Modes Brightfield  White LED light source  Eliminates heating of plates Darkfield  Changes the angle of incidence mitigating shadow regions associated with Brightfield  Assists visualization of crystals Polarization  True polarization  Variable angle transmitter and analyzer  Exploits birefringent property of crystals

7. 7 Lighting and treatment options  Brightfield  Darkfield  Variable Angle Polarization  Extinction  Extinction + 45°  Extinction + 90°

8. 8 Imaging Features  Drop-finding  X-focus (extended depth of focus)  Others: autofocus, autoexposure, sharpening

9. 9 Rhombix Software Suite – Supporting the Crystallization Workflow Results Analysis and Scoring Experiment Execution Experiment Design Data Mining Optimization Reagent Manager Screen Designer Experiment Manager Schedule Editor Barcode Manager Deck Manager Experiment Launcher Screen Monitor Vision Monitor Equipment Monitor Visual Query Tool Image Viewing and Annotation (Clarix) Report Tool Visual Query Tool Image Viewing and Annotation (Clarix) Screen Designer Protein Crystallization Software Packages Knowledge

10. 10 Rhombix Software Fourteen Software Applications Built into an Cohesive Framework Spanning: Reagent Management to Data-Mining User- defined values

11. 11 Viewing and Annotation -- Clarix  interface for viewing and annotating images  brings in information from database (e.g. well contents & drop details)  provides feedback to imaging  link to Screen Design for optimization screens  link to data-mining  link to scheduling

12. 12 Report Tool  Report across users and experiments  Export to.xls,.pdf, HTML, xml formats  One means of data sharing between sites (export at one site, import at another site)

13. 13 Data Mining – Visual Query Tool  Graphical data-mining SW for general queries and experiment optimization planning  Links to Viewing application for display of image results

14. 14 Screen Designer  Graphical interface for designing screens  Allows concentration and pH gradients  Gradients across grids, rows, columns  Patterns of start/step, start/end, center/step

15. 15 Import Utility  Imports chemicals, screening solution formulations, screens, and experiments  Pair with report tool for data sharing

16. 16 More Insight and Less Mystery in Crystallization Tools for easy data entry Tools for easy data retrieval and viewing “Drop to Crystal” Workflow