2. AP Biology 2007-2008 Prokaryotes Domain Bacteria Domain Archaebacteria Domain Bacteria Domain Archaea Domain Eukarya Common ancestor

3. What characteristics make Archaea more like eukaryotes than prokaryotes?  No peptidoglycan in cell wall  More than 1 RNA polymerase  Some introns  Met is 1 st amino acid in protein synthesis  Ribosomes more like eukarytoes (Streptomycin does not kill archaea) Domain Bacteria Domain Archaea Domain Eukarya Common ancestor

5. Bacteria live EVERYWHERE!  Bacteria live in all ecosystems  on plants & animals  in plants & animals  in the soil  in depths of the oceans  in extreme cold  in extreme hot  in extreme salt  on the living  on the dead Microbes always find a way to make a living!

6. Bacterial diversity rods and spheres and spirals

7. Prokaryote Structure  Unicellular  bacilli, cocci, spirilli  Size  1/10 size of eukaryote cell  1 micron (1um)  Internal structure: prokaryote cell eukaryote cell

8. Prokaryote vs. Eukaryote Chromosome double helix Prokaryote Eukaryote

9. Genetic variation in bacteria  Mutations  bacteria can reproduce every 20 minutes  binary fission  error rate in copying DNA  1 in every 200 bacteria has a mutation  you have billions of E. coli in your gut!  lots of mutation potential!  Genetic recombination  bacteria swap genes  small supplemental circles of DNA  direct transfer of DNA conjugation

10. Metabolic Relation to Oxygen  How are bacteria categorized by the effect that oxygen has on growth?  Obligate aerobes  Facultative anaerobes  Obligate anaerobes  What role do prokaryotes play in nitrogen cycling?  Only way that nitrogen gas can be incorporated into organic molecules

11. Variations in Cell Interior internal membranes for photosynthesis like a chloroplast (thylakoids) internal membranes for respiration like a mitochondrion (cristae) aerobic bacterium mitochondria cyanobacterium (photosythetic) bacterium chloroplast

12. Origins of Metabolic Diversity  Heterotrophs most likely came before photoautotrophs (parsimony)  Glycolysis was probably the first metabolic pathway  Natural selection favored autotrophs as heterotrophs depleted food supply  Cyanobacteria introduce chl a and oxygen gas.

13. Cell Wall  What are the functions of the cell wall in prokaryotes?  Maintain cell shape  Protection  Prevent bursting in hypotonic environment  Made of peptidoglycan  Why is some food preserved by salting it?  Hypertonic environment plasmolyzes bacterial cells

14. Prokaryote Cell Wall Structure peptide side chains cell wall peptidoglycan plasma membrane protein Gram-positive bacteria Gram-negative bacteria peptidoglycan plasma membrane outer membrane outer membrane of lipopolysaccharides cell wall peptidoglycan = polysaccharides + amino acid chains lipopolysaccharides = lipids + polysaccharides That’s important for your doctor to know!

15. Prokaryotic metabolism  How do bacteria acquire their energy & nutrients?  photoautotrophs  photosynthetic bacteria  chemoautotrophs  oxidize inorganic compounds  nitrogen, sulfur, hydrogen…  heterotrophs  live on plant & animal matter  decomposers & pathogens

17. Disease  Disease –  exotoxins release poisons such as botulism and cholera  Endotoxins seen in outer membrane of gram neg. bacteria (Salmonella)  What are Koch’s postulates and why are they important?  Koch’s postulates used to isolate pathogen causing disease

18. Bacteria as pathogens  Disease-causing microbes  plant diseases  wilts, fruit rot, blights  animal diseases  tooth decay, ulcers  anthrax, botulism  plague, leprosy, “flesh-eating” disease  STDs: gonorrhea, chlamydia  typhoid, cholera  TB, pneumonia  lyme disease

19. Bacteria as beneficial (& necessary)  Life on Earth is dependent on bacteria  recycling of nutrients from dead to living  only organisms that can fix N from atmosphere  needed for synthesis of proteins & nucleic acids  plant root nodules  help in digestion (E. coli)  digest cellulose for herbivores  cellulase enzyme  produce vitamins K & B 12 for humans  produce foods & medicines  from yogurt to insulin

21. Germinating akinetes

22. AP Biology 2007-2008 Got any Questions?? Ask da’ Boss!

24. Are these bacteria Gram + or Gram –? Gram positive Gram negative