1. Cell Structure and Function
Introduction to Cells
Cell Structure and Function
Cell biology is the study of the structure, function and diversity of cells.
How do cells grow, divide, specialize and differentiate, communicate and interact?

2. Agenda
What do you need to know about cells?
Cell Stories Project

3. Cells are the fundamental units of life.

4. What do you need to know about cells?
Evolutionary Perspective
Types of Cells--Prokaryotes and Eukaryotes
Cell Structure and Organization

5. Precursor to the first eukaryotic cell
The Tree of Life
The First Cell
Prokaryotes
Precursor to the first eukaryotic cell
Archaea
Bacteria
(Methanogens, Halophiles, Sulfolobus, and relatives)
(bacteria, mitochondria, and chloroplasts)
Eukarya
Eukaryotes
(Protists, Plants, Fungi, Animals)

6. 10-15 billion years ago:
4.5 billion years ago: Origin of the Solar System
3.5 to 4 billion years ago: Oldest bacterial fossils
2.7 billion years ago: First protists-the earliest eukaryotic cells.
1 billion years ago: First animals (earliest multicellular organisms) The blastula is the definitive characteristic of animal species.
0.5 billion years ago: First plants and fungi

7. Archaea
Dr. Carl Woese

8. Archaea
“The archaea are unique organisms. While prokaryotes in the cytological sense, they are actually more closely related to eukaryotes than to the bacteria. They are of particular interest for this reason alone-they are simple organisms whose study should provide insights into the nature and evolution of the eukaryotic cell.”

9. Our view of life's place of origin may soon shift from that long-favored "warm little pond" to undersea hydrothermal vents.
extremeophiles

10. Bacteria
E. coli is the most common bacterium in the human gut.

11. Eukarya
Plants
Animals
Fungi
Protists
2.7 billion years ago
Eukaryotes

12. Giardia lacks mitochondia, but contains two nuclei.
Protists
Tetrahymena
Amoeba
Giardia
SEM thanks to Stanley L. Erlandsen, and Dennis E. Feely, authors of "Trophozoite Motility and the Mechanism of Attachment," in Giardia and Giardiasis, Plenum Press, 1984
Giardia lacks mitochondia, but contains two nuclei.

13. Fungi (yeasts, molds and mushrooms)
morels
The Michigan fungus is estimated to be 1500 years old. It covers about 38 acres and weighs about 11,000 kg.
Number of fungal species: about 65,000.

14. How Cells Work

15. Plasma Membrane

16. Cell Structure and Organization

17. The Nucleus
-Composed of two concentric membranes that form the nuclear envelope.
-Contains a nucleolus
-How did the nucleus evolve?

18. Nuclear pores are sites where the inner and outer membranes of the nuclear envelope are joined.
The nuclear envelope has pores and is continuous with the ER

19. Ribosomes are produced in the nucleolus
Ribosomes are produced in the nucleolus. This is where ribosomal RNA is transcribed.

20. Nuclear Pores
Nuclear pores allow RNA and protein to move in and out of the nucleus.
How does the cell know which proteins are transported into the nucleus?

21. Nuclear Transport
Proteins targeted for the nucleus have a signal sequence called the nuclear localization signal
Nuclear import receptors bind to the signal sequence and direct it to the pore
Energy from the hydrolysis of GTP is needed to transport proteins into the nucleus

23. Endoplasmic Reticulum (ER)
Cell membrane components and materials to be exported from the cell are made here.
Transports proteins to be compartmentalized within the cell or exported out of the cell
Ribosomes attached to the ER can recognize a signal sequence on a protein and transport that protein to the ER

24. Endoplasmic Reticulum (ER)

25. Material is transported between the ER and the Golgi
in transport vesicles.
George Palade
Figure (modified) from A Textbook of Histology by D Fawcett. Chapman and Hall, N.Y. 1994

26. The Golgi Apparatus
Synthesis and modification of molecules to be secreted from the cell
Directs proteins made in the ER to the correct cellular compartment or to the plasma membrane for secretion

27. Mitochondria are the energy factories of the cell
Two membranes
Maternally inherited
Contain their own DNA and replicate
Related to bacteria
How did they evolve?

28. Lysosomes are vesicles that degrade cell debris.
Figure (modified) taken from Molecular Biology of the Cell by Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson, Garland Publishing, NY 1994

29. Chloroplasts

30. Chloroplasts Composed of internal membranes with distinct functions
Using energy from sunlight, convert carbon dioxide and water to sugar (photosynthesis)
Contain their own DNA and replicate by dividing
Like bacteria in composition
How did they evolve?

31. Cellular Architecture
The cytoskeleton is a fibrous network within the cytoplasm that maintains cell shape, allows movement of materials within cells and assists in cell motility.
Only eukaryotic cells have a cytoskeleton.
The cytoskeleton consists of three component filaments: microfilaments, microtubules and intermediate filaments.

32. The Cytoskeleton
Actin filaments are part of the machinery that generates contractile forces (large numbers in muscle cells)
Microtubules help pull duplicated chromosomes apart
Intermediate filaments give the cell mechanical strength and controls shape

33. Actin Microtubules Intermediate

34. Cell Structure and Organization
Cilia and flagella are projections from the cell, made of microtubules.

35. The Extracellular Matrix
The extracellular matrix is connective tissue that holds the cells of a tissue together. It can be solid, gelatinous or liquid.

36. Prokaryotes No nucleus (defining characteristic) No organelles
No cytoskeleton
Single cell organisms
Smaller and simpler than eukaryotic cells

37. Eucaryotes

38. Evolution of Mitochondria

39. Chloroplast Evolution