1. BIODIVERSITY I BIOL 1051 What are Eukaryotic Microorganisms? Professor Marc C Lavoie mlavoie@uwichill.edu.bb

2. MICRO-ORGANISMS PROKARYOTES BACTERIAARCHAEA EUKARYOTES AMOEBOZOAOPISTOKONTARHIZARIAARCHAEPLASTIDACHROMALVEOLATAEXCAVATA VIRUSES

3. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

4. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

5. 1.Eukaryotic Microorganisms are eukaryotic cells

6. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

7. 2. Eukaryotic Microorganisms are microscopic

8. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

9. 3.Eukaryotic Microorganisms could be unicellular or pluricellular

10. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

11. 4.Size and shape of Eukaryotic Microorganisms

12. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

13. 5. Eukaryotic Microorganisms structures Characteristic Prokaryote Eukaryote a. ChromosomeSingle circular Paired linear b. Chromosome location Nucleoid (no membrane) Nucleus (membrane present) c. Nucleolus Absent Present d. Extra chromosomal DNA Plasmid Mitochondria and Chloroplast e. Site of Cellular Respiration Cell membrane Mitochondria f. Ribosomes 30S & 50S /70 S40S & 60S /80 S in cytoplasm (70 S in organelles) g. LocomotionRotating flagella and gliding Undulating flagella and cilia, and also amoeboid movement h. Pili Sex or attachment pili Absent

14. 5.1 Cytoplasmic membrane

15. 5.2 Cell wall

16. 5.3 Chromosomes

17. 5.4 Ribosomes 40S 60S 80S

18. 5.4 Ribosomes

19. 5.5 Nucleus

20. 5.6 RNA-polymerase Eukaryotes more complex than Prokaryotes

21. BacteriaArchaeaEukarya

22. 5.7 Cilia and flagella

23. 5.8 Mitochondria

24. FlatTubularDiscoid

25. 5.8 Chloroplast

26. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

27. 6. Eukaryotic Microorganisms multiplication

29. Budding

30. 6. Eukaryotic Microorganisms life cycles

31. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

32. 7. Diversity of Eukaryotic Microorganisms metabolism ENERGY SOURCEELECTRON DONNORCARBON SOURCE ORGANISMS PROKARYOTES AUTO- ALGAE (CO 2 )CERTAIN PROTISTS LITHO- Plants PHOTO- (Light) (Inorganic compounds and C 1 ) HETERO- (CH 2 O) n None ORGANO-AUTO- None (Organic compounds) HETERO-Prokaryotes AUTO-Prokaryotes LITHO-HETERO- Prokaryotes CHEMO- (Chemical compounds) AUTO- Prokaryotes ORGANO- Prokaryotes MOST PROTISTS HETERO-FUNGI Plants (non-chlorophyllic) Animals

33. What are Eukaryotic Microorganisms? 1.Eukaryotic Microorganisms are eukaryotic cells 2.Eukaryotic Microorganisms are microscopic 3.Eukaryotic Microorganisms are unicellular or pluricellular 4.Size and shape of Eukaryotic Microorganisms 5. Eukaryotic Microorganisms structures 6.Eukaryotic Microorganisms multiplication 7. Diversity of Eukaryotic Microorganisms metabolism 8.Eukaryotic Microorganisms diversity

35. Adl et al 2005

36. Embley & Martin 2006

37. MICRO-ORGANISMS PROKARYOTES BACTERIAARCHAEA EUKARYOTES AMOEBOZOAOPISTOKONTARHIZARIAARCHAEPLASTIDACHROMALVEOLATAEXCAVATA VIRUSES

38. Amoeboid locomotion generally with non-eruptive morphologically variable pseudopodia (lobopodia) AMOEBOZOA

39. MICRO-ORGANISMS PROKARYOTES BACTERIAARCHAEA EUKARYOTES AMOEBOZOAOPISTOKONTARHIZARIAARCHAEPLASTIDACHROMALVEOLATAEXCAVATA VIRUSES

40. OPISTOKONTA Single posterior cilium without mastigonemes FUNGI, CHOANOZOA, AND ANIMALS

41. OPISTOKONTA FUNGI CHOANOZOA ANIMALS

42. MICRO-ORGANISMS PROKARYOTES BACTERIAARCHAEA EUKARYOTES AMOEBOZOAOPISTOKONTARHIZARIAARCHAEPLASTIDACHROMALVEOLATAEXCAVATA VIRUSES

43. RHIZARIA With fine pseudopodia (filopodia) varying as simple, branching, anastomosing, or supported by microtubules (axopodia).

44. MICRO-ORGANISMS PROKARYOTES BACTERIAARCHAEA EUKARYOTES AMOEBOZOAOPISTOKONTARHIZARIAARCHAEPLASTIDACHROMALVEOLATAEXCAVATA VIRUSES

45. ARCHAEPLASTIDA Photosynthetic plastid with chlorophyll a from an ancestral primary endosymbiosis with a cyanobacterium –plastid secondarily lost or reduced in some –usually with cellulose cell wall –flat cristae –storage product: starch GREEN ALGAE AND PLANTS

46. ALGAE

47. GREEN ALGAE AND PLANTS ARCHAEPLASTIDA

48. MICRO-ORGANISMS PROKARYOTES BACTERIAARCHAEA EUKARYOTES AMOEBOZOAOPISTOKONTARHIZARIAARCHAEPLASTIDACHROMALVEOLATAEXCAVATA VIRUSES

49. CHROMALVEOLATA Plastid from secondary endosymbiosis with an ancestral archaeplastida

50. ALVEOLATES

51. CHROMALVEOLATA Dinoflagellates Apicomplexa Ciliates

52. A typical ciliate Paramecium

53. A flagellate: Trypanosoma

54. CHROMISTA Diatoms Brown algae Oomycetes

55. MICRO-ORGANISMS PROKARYOTES BACTERIAARCHAEA EUKARYOTES AMOEBOZOAOPISTOKONTARHIZARIAARCHAEPLASTIDACHROMALVEOLATAEXCAVATA VIRUSES

56. EXCAVATA Typically with suspension-feeding groove (cytostome) of the ‘‘excavate’’ type (i.e. homologous to that in Jakoba libera), presumed to be secondarily lost in many taxa

57. Lecture 3: What are Eukaryotic Microorganisms? REFERENCE: MADIGAN, MT, MARTINKO, JM, & PARKER, J. Brock Biology of Microorganisms, 10 th ed, 2003, PRENTICE HALL, p.472-498. PURVES, WK, SADAVA, D, ORIANS, GH, HELLER, HC. Life, The Science of Biology, 6th ed, 2001, Sinauer Assiciates Inc., p. 476-499.

58. Lecture 3: What are Eukaryotic Microorganisms? WEB Sites: http://sciences.aum.edu/bi/BI2033/thomson/binar yfission.htmlhttp://sciences.aum.edu/bi/BI2033/thomson/binar yfission.html http://cwx.prenhall.com/bookbind/pubbooks/brock2/ chapter17/deluxe.htmlhttp://cwx.prenhall.com/bookbind/pubbooks/brock2/ chapter17/deluxe.html http://www.slic2.wsu.edu:82/hurlbert/micro101/p ages/101lab1.htmlhttp://www.slic2.wsu.edu:82/hurlbert/micro101/p ages/101lab1.html http://www.liunet.edu/cwis/bklyn/acadres/facdev/Fa cultyProjects/WebClass/micro-web/html- files/chapterA-1.htmlhttp://www.liunet.edu/cwis/bklyn/acadres/facdev/Fa cultyProjects/WebClass/micro-web/html- files/chapterA-1.html

59. Lecture 3: What are Eukaryotic Microorganisms? WEB Sites: http://www.biosci.uga.edu/almanac/bio_103/notes/may_ 15.htmlhttp://www.biosci.uga.edu/almanac/bio_103/notes/may_ 15.html www.DennisKunkel.com http://gened.emc.maricopa.edu/bio/BIO181/BIOBK/Plantce ll.gifhttp://gened.emc.maricopa.edu/bio/BIO181/BIOBK/Plantce ll.gif http://gened.emc.maricopa.edu/bio/BIO181/BIOBK/BioBoo kCELL2.html#The nucleus http://mbclserver.rutgers.edu/~sofer/genetics/Transcription/ Transcription7.htmlhttp://mbclserver.rutgers.edu/~sofer/genetics/Transcription/ Transcription7.html www.sinauer.com

60. Lecture 3: What are Eukaryotic Microorganisms? WEB Sites: www.whfreeman.com http://tolweb.org/tree/phylogeny.html http://ag.arizona.edu/tree/life.html http://pearl.spatial.maine.edu/pictures/glossary/A LGAE.jpghttp://pearl.spatial.maine.edu/pictures/glossary/A LGAE.jpg